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Gene Expression Data
Assay Details
Assay
Reference: J:312380 Sun-Wada GH, et al., Vacuolar-type proton ATPase is required for maintenance of apicobasal polarity of embryonic visceral endoderm. Sci Rep. 2021 Sep 29;11(1):19355
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7541115
Gene symbol: Prkcz
Gene name: protein kinase C, zeta
Antibody: anti-PKCzeta
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 2a/b/c: embryonic day 5.5 (more )
Note: Triple labeled: green - Prkcz; red - Cdh1 (assay MGI:7541114); blue - Gata6 (assay MGI:7541116).
Structure Level Pattern Image Note
TS7: primitive endoderm Present 2a , 2c , 2b Enriched in the apical region of visceral endoderm cells.
Specimen 2e/f/g: embryonic day 5.5; Atp6v0ctm1.1Ywd/Atp6v0ctm1.1Ywd (more )
Note: Triple labeled: green - Prkcz; red - Cdh1 (assay MGI:7541114); blue - Gata6 (assay MGI:7541116).
Structure Level Pattern Image Note
TS7: primitive endoderm Present 2f , 2e , 2g More broadly distributed in the cells. The apical surface became rather spherical than flat.
Specimen S3a WT: embryonic day 3.5 (more )
Note: Triple labeled: green - Prkcz; blue - Gata6 (assay MGI:7541116); red - Sox17 (assay MGI:7541133).
Structure Level Pattern Image Note
TS4: inner cell mass Present S3a WT right Enriched in the apical region.
TS4: trophectoderm Present S3a WT right Enriched in the apical region.
Specimen S3a -/-: embryonic day 3.5; Atp6v0ctm1.1Ywd/Atp6v0ctm1.1Ywd (more )
Note: Triple labeled: green - Prkcz; blue - Gata6 (assay MGI:7541116); red - Sox17 (assay MGI:7541133).
Structure Level Pattern Image Note
TS4: inner cell mass Present S3a -/- right Enriched in the apical region, similar as in the wild-type.
TS4: trophectoderm Present S3a -/- right Enriched in the apical region, similar as in the wild-type.
Specimen S3b WT: embryonic day 4.5 (more )
Note: Triple labeled: green - Prkcz; blue - Gata6 (assay MGI:7541116); red - Sox17 (assay MGI:7541133).
Structure Level Pattern Image Note
TS6: primitive endoderm Present S3b WT right Enriched in the apical region of visceral endoderm cells.
Specimen S3b -/-: embryonic day 4.5; Atp6v0ctm1.1Ywd/Atp6v0ctm1.1Ywd (more )
Note: Triple labeled: green - Prkcz; blue - Gata6 (assay MGI:7541116); red - Sox17 (assay MGI:7541133).
Structure Level Pattern Image Note
TS6: primitive endoderm Present S3b -/- right Enriched in the apical region, similar as in the wild-type.
Specimen S3c WT: embryonic day 5.0 (more )
Note: Triple labeled: green - Prkcz; blue - Gata6 (assay MGI:7541116); red - Sox17 (assay MGI:7541133).
Structure Level Pattern Image Note
TS7: embryonic visceral endoderm Present S3c WT right Enriched in the apical region of visceral endoderm cells.
Specimen S3c -/-: embryonic day 5.0; Atp6v0ctm1.1Ywd/Atp6v0ctm1.1Ywd (more )
Note: Triple labeled: green - Prkcz; blue - Gata6 (assay MGI:7541116); red - Sox17 (assay MGI:7541133).
Structure Level Pattern Image Note
TS7: embryonic visceral endoderm Present S3c -/- right More broadly distributed in the cells.
Specimen S3d WT: embryonic day 5.5 (more )
Note: Triple labeled: green - Prkcz; blue - Gata6 (assay MGI:7541116); red - Sox17 (assay MGI:7541133).
Structure Level Pattern Image Note
TS7: embryonic visceral endoderm Present S3d WT right Enriched in the apical region of visceral endoderm cells.
Specimen S3d -/-: embryonic day 5.5; Atp6v0ctm1.1Ywd/Atp6v0ctm1.1Ywd (more )
Note: Triple labeled: green - Prkcz; blue - Gata6 (assay MGI:7541116); red - Sox17 (assay MGI:7541133).
Structure Level Pattern Image Note
TS7: embryonic visceral endoderm Present S3d -/- right More broadly distributed in the cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
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