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Gene Expression Data
Assay Details
Assay
Reference: J:324832 Sun Y, et al., Single-cell transcriptomic landscapes of the otic neuronal lineage at multiple early embryonic ages. Cell Rep. 2022 Mar 22;38(12):110542
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7493524
Gene symbol: Neurod1
Gene name: neurogenic differentiation 1
Antibody: anti-NeuroD1 (3H8; ab60704)
Detection system: Secondary antibody
Results
Specimen S1C: embryonic day 9.5; Foxg1tm1.1(cre)Ddmo/Foxg1+, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Multi-labeled: white - Neurod1; green - Phox2b (assay MGI:7493529); red - Cre reporter; blue - Hoechst.
Structure Level Pattern Image Note
TS16: cranial ganglion Present Regionally restricted S1E'' , S1C'' , S1D''' , S1E''' , S1D'' , S1C''' Expression is seen in cells committed to be cochleo-vestibular ganglion cells and geniculate ganglion cells.
Specimen 2A/B: embryonic day 9.5 (more )
Note: Triple labeled: red - Neurod1; green - Gadd45g (assay MGI:7493517); blue - Hoechst.
Structure Level Pattern Image Note
TS16: cranial ganglion Present Regionally restricted 2B' , 2A' , 2B'' , 2A'' Expression is seen in cells committed to be cochleo-vestibular ganglion cells.
TS16: otocyst epithelium Present 2B' , 2B'' , 2A' , 2A''
Specimen 2C/D: embryonic day 9.5 (more )
Note: Triple labeled: red - Neurod1; green - Miat (assay MGI:7493518); blue - Hoechst.
Structure Level Pattern Image Note
TS16: cranial ganglion Present Regionally restricted 2C' , 2D' , 2D'' , 2C'' Expression is seen in cells committed to be cochleo-vestibular ganglion cells.
TS16: otocyst epithelium Present 2C'' , 2D'' , 2D' , 2C'
Specimen 2E/F: embryonic day 9.5 (more )
Note: Triple labeled: red - Neurod1; green - Hes6 (assay MGI:7493519); blue - Hoechst.
Structure Level Pattern Image Note
TS16: cranial ganglion Present Regionally restricted 2E' , 2F' , 2E'' , 2F'' Expression is seen in cells committed to be cochleo-vestibular ganglion cells.
TS16: otocyst epithelium Present 2E'' , 2F'' , 2F' , 2E'
Specimen 2G/H: embryonic day 9.5 (more )
Note: Triple labeled: red - Neurod1; green - Tmsb4x (assay MGI:7493520); blue - Hoechst.
Structure Level Pattern Image Note
TS16: cranial ganglion Present Regionally restricted 2H' , 2G' , 2G'' , 2H'' Expression is seen in cells committed to be cochleo-vestibular ganglion cells.
TS16: otocyst epithelium Present 2H' , 2H'' , 2G' , 2G''
Specimen 4A/B: embryonic day 9.5 (more )
Note: Multi-labeled: white - Neurod1; green - Insm1 (assay MGI:7493531); red - Myt1 (assay MGI:7493605); blue - Hoechst.
Structure Level Pattern Image Note
TS16: cranial ganglion Present Regionally restricted 4B''' , 4A''' , 4A'' , 4B'' Expression is seen in cells committed to be cochleo-vestibular ganglion cells.
Specimen 4C: embryonic day 10.5 (more )
Note: Triple labeled: red - Neurod1; green - Myt1 (assay MGI:7493605); blue - Hoechst.
Structure Level Pattern Image Note
TS18: cranial ganglion Present Regionally restricted 4C' , 4C'' Expression is seen in cells committed to be cochleo-vestibular ganglion cells.
Specimen S8G/H: embryonic day 9.5; Shox2em1(cre)Zyliu/Shox2+ (more )
Note: Triple labeled: red - Neurod1; green - Shox2 reporter (assay MGI:7489979); blue - Hoechst.
Structure Level Pattern Image Note
TS16: cranial ganglion Present Regionally restricted S8G , S8G'' , S8H , S8H'' Expression is seen in cells committed to be cochleo-vestibular ganglion cells.
TS16: otocyst epithelium Present S8G'' , S8G
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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