Antibody:	Anti-YAP1 antibody [2F12] (ab56701)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Strong	Regionally restricted	1A right lower , 1A right upper , 1A middle right , 1A left lower , 1A middle left , 1A left upper	Highly expressed in proliferating ventricular zone labeled with Sox9, but was nearly undetectable in Sox9-negative postmitotic cells located closer to the pia membrane.
TS20: choroid plexus	Strong		1A left lower , 1A left upper
TS20: brain meninges	Strong		1A left upper , 1A right upper , 1A middle right , 1A left lower , 1A middle left , 1A right lower

Structure	Level	Pattern	Image	Note
TS24: cerebellum external granule cell layer	Strong		1B left , 1B right upper , 1B right lower Overlay , 1B right lower Yap	Coexpressed with Pax6.
TS24: cerebellum ventricular layer	Strong		1B left
TS24: choroid plexus	Strong		1B left
TS24: upper rhombic lip	Strong		1B left

Structure	Level	Pattern	Image	Note
TS24: cerebellum ventricular layer	Strong		1C left , 1C2 Overlay , 1C1 Yap , 1C1 Overlay , 1C2 Yap	Expressed in radial glia progenitors. Also expressed in radial glia processes (Fabp7+).
TS24: choroid plexus	Strong		1C left
TS24: hindbrain pia mater	Strong		1C left

Structure	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	Strong	Regionally restricted	1D left , 1D right Overlay , 1D middle , 1D right Yap	Coexpressed with Pax6 in the outer most layers of the external granule cell layer, limited to 1-2 cell layers from the meninges.
TS27: 4th ventricle ependyma	Present	Regionally restricted	1D left
TS27: cerebellum Purkinje cell layer	Present	Regionally restricted	1D left
TS27: cerebellum white matter	Present	Regionally restricted	1D left
TS27: choroid plexus	Present	Regionally restricted	1D left

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Bergmann glial cell	Strong		1E left , 1E right Overlay , 1E right Yap	Coexpressed with S100b in the soma, including the nucleus and radial processes.
TS28: cerebellum ventricular layer		Present		1E left
TS28: choroid plexus		Present		1E left

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	cerebellar granule cell precursor	Strong	Regionally restricted	1F left , 1F middle	Expressed in the outer most layers of the external granule cell layer.
TS28: cerebellum ventricular layer	Bergmann glial cell	Strong		1F left , 1F middle	Expressed in in the soma and processes.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Absent		1G middle , 1G left	Not coexpressed with Calb1.

Structure	Level	Pattern	Image	Note
TS21: cerebellum layer	Present	Regionally restricted	3A middle lower , 3A left , 3A middle upper	Expressed in the cerebellum external granule cell layer.
TS21: upper rhombic lip	Present		3A right upper , 3A right lower , 3A left
TS21: choroid plexus	Strong		3A left , 3A right lower , 3A right upper
TS21: hindbrain meninges	Strong		3A middle lower , 3A middle upper , 3A left

Structure	Level	Pattern	Image	Note
TS21: upper rhombic lip	Ambiguous		3B right upper , 3B left , 3B right lower	Nearly undetectable. Smaller size compared with the wild-type.
TS21: cerebellum layer	Ambiguous		3B middle upper , 3B left , 3B middle lower	Nearly undetectable.
TS21: choroid plexus	Strong		3B left , 3B right lower , 3B right upper
TS21: hindbrain meninges	Strong		3B left , 3B middle lower , 3B middle upper

Structure	Level	Pattern	Image	Note
TS24: cerebellum external granule cell layer	Strong		3J WT
TS24: choroid plexus	Strong		3J WT
TS24: hindbrain meninges	Strong		3J WT
TS24: upper rhombic lip	Strong		3J WT

Structure	Level	Pattern	Image	Note
TS24: choroid plexus	Strong		3J CKO
TS24: hindbrain meninges	Strong		3J CKO
TS24: cerebellum external granule cell layer	Ambiguous		3J CKO	Largely undetectable.
TS24: upper rhombic lip	Ambiguous		3J CKO	Largely undetectable. Reduced size compared with the wild-type.

Structure	Level	Pattern	Image	Note
TS27: cerebellum granule cell layer	Present		4B WT
TS27: brain blood vessel	Strong		4B WT
TS27: choroid plexus	Strong		4B WT
TS27: hindbrain meninges	Strong		4B WT

Structure	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	Absent		4B CKO
TS27: brain blood vessel	Strong		4B CKO
TS27: choroid plexus	Strong		4B CKO
TS27: hindbrain meninges	Strong		4B CKO

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	cerebellar granule cell precursor	Present	Regionally restricted	5A WT middle , 5A WT left , 5A WT right	Expressed in the outer most layers of the external granule cell layer.
TS27: cerebellum ventricular layer	Bergmann glial cell	Strong		5A WT middle , 5A WT left , 5A WT right
TS27: hindbrain meninges		Strong		5A WT left , 5A WT right , 5A WT middle
TS27: choroid plexus		Strong		5A WT left

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	cerebellar granule cell precursor	Absent		5A CKO left , 5A CKO right , 5A CKO middle
TS27: cerebellum ventricular layer	Bergmann glial cell	Strong		5A CKO middle , 5A CKO left , 5A CKO right
TS27: hindbrain meninges		Strong		5A CKO left , 5A CKO middle , 5A CKO right
TS27: choroid plexus		Strong		5A CKO left

Structure	Level	Pattern	Image	Note
TS21: cerebellum ventricular layer	Present		7A WT right Yap , 7A WT left	Expressed in radial glia progenitors.
TS21: choroid plexus	Strong		7A WT left

Structure	Level	Pattern	Image	Note
TS21: cerebellum ventricular layer	Ambiguous		7A CKO right Yap , 7A CKO left	Nearly undetectable expression in the radial glia progenitors.
TS21: choroid plexus	Strong		7A CKO left

Structure	Level	Pattern	Image	Note
TS23: cerebellum ventricular layer	Present		7B WT middle Yap , 7B WT middle Overlay , 7B WT left	Expressed in radial glia progenitors.
TS23: choroid plexus	Strong		7B WT left
TS23: hindbrain meninges	Strong		7B WT left

Structure	Level	Pattern	Image	Note
TS23: cerebellum ventricular layer	Trace		7B CKO middle Yap , 7B CKO left , 7B CKO middle Overlay
TS23: choroid plexus	Strong		7B CKO left
TS23: hindbrain meninges	Strong		7B CKO left

Structure	Level	Pattern	Image	Note
TS22: cerebellum ventricular layer	Present		8K

Structure	Level	Pattern	Image	Note
TS22: cerebellum ventricular layer	Present	Regionally restricted	8L	Due to the mosaic Cre expression, expression was partially lacking.

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Strong	Graded	S1A left , S1A middle	Expressed in a graded distribution (anterior high and posterior low).
TS27: brain blood vessel	Present		S1A middle , S1A left
TS27: hindbrain meninges	Present		S1A middle , S1A left

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum ventricular layer	Bergmann glial cell	Present		S1B left upper , S1B left lower , S1B right upper , S1B right lower	Extensively coexpressed with Gfap in the processes.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	cerebellar granule cell	Absent		S1C right , S1C left	No expression in postmitotic cerebellar granule cells.
TS28: cerebellum external granule cell layer	Bergmann glial cell	Weak		S1C right , S1C left	Weak expression in Bergman glia cell fibers remained.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cerebellum	Bergmann glial cell	Strong		S4A , S4A'
TS27: choroid plexus		Strong		S4A , S4A'
TS27: hindbrain meninges		Strong		S4A , S4A'

Structure	Cell Type	Level	Pattern	Image	Note
TS27: brain blood vessel		Strong		S4B , S4B'
TS27: choroid plexus		Strong		S4B , S4B'
TS27: hindbrain meninges		Strong		S4B , S4B'
TS27: cerebellum	Bergmann glial cell	Absent		S4B , S4B'

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Bergmann glial cell	Present		S5A , S5A'
TS28: choroid plexus		Strong		S5A' , S5A
TS28: hindbrain meninges		Strong		S5A' , S5A

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Bergmann glial cell	Absent		S5B' , S5B
TS28: choroid plexus		Strong		S5B' , S5B
TS28: hindbrain meninges		Strong		S5B' , S5B

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	cerebellar granule cell precursor	Present		S6A
TS28: cerebellum Purkinje cell layer	Bergmann glial cell	Present		S6A

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	cerebellar granule cell precursor	Present		S6B	Similar as in the wild-type.
TS28: cerebellum Purkinje cell layer	Bergmann glial cell	Present		S6B	Similar as in the wild-type.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum vermis lobule IX	Bergmann glial cell	Present		S6E , S6E'
TS28: brain blood vessel		Present		S6E , S6E'
TS28: hindbrain meninges		Present		S6E , S6E'

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum vermis lobule IX	Bergmann glial cell	Weak		S6F , S6F'	Reduced expression compared with the wild-type.
TS28: brain blood vessel		Present		S6F , S6F'	Similar as in the wild-type.
TS28: hindbrain meninges		Present		S6F , S6F'	Similar as in the wild-type.

Structure	Level	Pattern	Image	Note
TS24: cerebellum ventricular layer	Present		S7A , S7A'
TS24: brain blood vessel	Strong		S7A
TS24: choroid plexus	Strong		S7A
TS24: hindbrain meninges	Strong		S7A

Structure	Level	Pattern	Image	Note
TS24: cerebellum ventricular layer	Ambiguous		S7B , S7B'	Expression was diminished.
TS24: brain blood vessel	Strong		S7B
TS24: choroid plexus	Strong		S7B
TS24: hindbrain meninges	Strong		S7B