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Gene Expression Data
Assay Details
Assay
Reference: J:283394 Hughes LJ, et al., Yap/Taz are required for establishing the cerebellar radial glia scaffold and proper foliation. Dev Biol. 2020 Jan 1;457(1):150-162
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7434455
Gene symbol: Fabp7
Gene name: fatty acid binding protein 7, brain
Antibody: Anti-Brain lipid binding protein Antibody (ABN14)
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 1C: embryonic day 16.5 (more )
Note: Double labeled: red - Fabp7; green - Yap1 (assay MGI:7434448).
Structure Level Pattern Image Note
TS24: cerebellum ventricular layer Present 1C left , 1C2 BLBP , 1C1 BLBP , 1C2 Overlay , 1C1 Overlay Expressed in radial glia progenitors.

Specimen 1F: postnatal day 5 (more )
Note: Double labeled: red - Fabp7; green - Yap1 (assay MGI:7434448).
Structure Level Pattern Image Note
TS28: cerebellum ventricular layer Present 1F left , 1F right Expressed in radial glia progenitors.

Specimen 7A WT: embryonic day 13.5 (more )
Note: Double labeled: red - Fabp7; green - Yap1 (assay MGI:7434448).
Structure Level Pattern Image Note
TS21: cerebellum ventricular layer Present 7A WT left , 7A WT right BLBP Expressed in radial glia progenitors, which were tightly arranged in a few cell layers along the ventricular lining.

Specimen 7A dCKO: embryonic day 13.5; Tg(Nes-cre)1Atp/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant. Double labeled: red - Fabp7; green - Yap1 (assay MGI:7434448).
Structure Level Pattern Image Note
TS21: cerebellum ventricular layer Present 7A CKO right BLBP , 7A CKO left Expressed in radial glia progenitors, which were considerably dispersed and less tightly arranged as compared to the wild-type. Some were displaced basally and some were maintained in the ventricular surface.

Specimen 7B WT: embryonic day 15.5 (more )
Note: Double labeled: red - Fabp7; green - Yap1 (assay MGI:7434448).
Structure Level Pattern Image Note
TS23: cerebellum ventricular layer Present 7B WT middle BLBP , 7B WT right , 7B WT left , 7B WT middle Overlay Expressed in radial glia progenitors. Dorsally directed processes were seen.

Specimen 7B dCKO: embryonic day 15.5; Tg(Nes-cre)1Atp/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant. Double labeled: red - Fabp7; green - Yap1 (assay MGI:7434448).
Structure Level Pattern Image Note
TS23: cerebellum ventricular layer Present 7B CKO left , 7B CKO middle BLBP , 7B CKO middle Overlay , 7B CKO right Reduced number of positive radial glia progenitors and radial glia processes were randomly distributed. Cell bodies were abnormally elongated in a horizontal rather than vertical plane along the ventricular surface and also situated aberrantly in basal locations.

Specimen 7C WT: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: cerebellum ventricular layer Present 7C WT Expressed in radial glia progenitors.

Specimen 7C dCKO: embryonic day 16.5; Tg(Nes-cre)1Atp/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS24: cerebellum ventricular layer Present Regionally restricted 7C CKO The number of positive radial glia progenitors was comparable to that of wild type despite the absence of positive ventricular lining cells.

Specimen 7E WT: postnatal day 0 (more )
Structure Cell Type Level Pattern Image Note
TS27: cerebellum Purkinje cell layer Bergmann glial cell Present 7E WT Cell bodies were mostly clustered at the Purkinje cell layer.

Specimen 7E dCKO: postnatal day 0; Tg(Nes-cre)1Atp/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS27: cerebellum external granule cell layer Bergmann glial cell Present 7E CKO More cell bodies were found.
TS27: cerebellum molecular layer Bergmann glial cell Present 7E CKO More cell bodies were found.

Specimen 7F WT: postnatal day 7 (more )
Structure Cell Type Level Pattern Image Note
TS28: cerebellum fissure Bergmann glial cell Present 7F WT

Specimen 7F dCKO: postnatal day 7; Tg(Nes-cre)1Atp/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: cerebellum fissure Bergmann glial cell Present 7F CKO Less organized cell bodies compared with the wild-type.

Specimen S4A: postnatal day 0 (more )
Note: Double labeled: red - Fabp7; green - Yap1 (assay MGI:7434448).
Structure Cell Type Level Pattern Image Note
TS27: cerebellum Bergmann glial cell Present S4A' , S4A

Specimen S4B: postnatal day 0; Tg(Nes-cre)1Atp/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant. Double labeled: red - Fabp7; green - Yap1 (assay MGI:7434448).
Structure Cell Type Level Pattern Image Note
TS27: cerebellum Bergmann glial cell Present S4B' , S4B

Specimen S4C/C': postnatal day 0 (more )
Note: Double labeled: red - Fabp7; green - Calb1 (assay MGI:7434575).
Structure Cell Type Level Pattern Image Note
TS27: cerebellum Purkinje cell layer Bergmann glial cell Present S4C' , S4C

Specimen S4D/D': postnatal day 0; Tg(Nes-cre)1Atp/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant. Double labeled: red - Fabp7; green - Calb1 (assay MGI:7434575).
Structure Cell Type Level Pattern Image Note
TS27: cerebellum Purkinje cell layer Bergmann glial cell Present S4D , S4D'
TS27: cerebellum external granule cell layer Bergmann glial cell Present S4D , S4D'

Specimen S7A/A': embryonic day 16.5 (more )
Note: Double labeled: red - Fabp7; green - Yap1 (assay MGI:7434448).
Structure Level Pattern Image Note
TS24: cerebellum ventricular layer Present S7A , S7A' Expressed in radial glia progenitors.

Specimen S7B/B': embryonic day 16.5; Tg(GFAP-cre)25Mes/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant. Double labeled: red - Fabp7; green - Yap1 (assay MGI:7434448).
Structure Level Pattern Image Note
TS24: cerebellum ventricular layer Present S7B , S7B' Expressed in radial glia progenitors, whose shapes were not radially polarized.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/21/2024
MGI 6.23
The Jackson Laboratory