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Gene Expression Data
Assay Details
Assay
Reference: J:283394 Hughes LJ, et al., Yap/Taz are required for establishing the cerebellar radial glia scaffold and proper foliation. Dev Biol. 2020 Jan 1;457(1):150-162
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7434454
Gene symbol: Pax6
Gene name: paired box 6
Antibody: anti-Pax6 (#PRB-278P; #30130, #901301)
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 1B: embryonic day 16.5 (more )
Note: Double labeled: red - Pax6; green - Yap1 (assay MGI:7434448).
Structure Level Pattern Image Note
TS24: cerebellum external granule cell layer Present 1B right lower Overlay , 1B right lower Pax6 , 1B left , 1B right upper
Specimen 1D: postnatal day 0 (more )
Note: Double labeled: red - Pax6; green - Yap1 (assay MGI:7434448).
Structure Level Pattern Image Note
TS27: cerebellum external granule cell layer Present 1D middle , 1D left , 1D right Pax6 , 1D right Overlay
Specimen 3A: embryonic day 13.5 (more )
Note: Double labeled: red - Pax6; green - Yap1 (assay MGI:7434448).
Structure Level Pattern Image Note
TS21: cerebellum layer Present Regionally restricted 3A left , 3A middle upper Expressed in the cerebellum external granule cell layer.
TS21: upper rhombic lip Present 3A left , 3A right upper
Specimen 3B: embryonic day 13.5; Tg(Nes-cre)1Atp/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant. Double labeled: red - Pax6; green - Yap1 (assay MGI:7434448).
Structure Level Pattern Image Note
TS21: cerebellum layer Present Regionally restricted 3B left , 3B middle upper Expressed in the cerebellum external granule cell layer.
TS21: upper rhombic lip Present 3B left , 3B right upper Smaller size compared with the wild-type.
Specimen 3C: embryonic day 13.5 (more )
Note: Double labeled: red - Pax6; green - BrdU.
Structure Level Pattern Image Note
TS21: cerebellum layer Present Regionally restricted 3C Expressed in the cerebellum external granule cell layer. The majority of positive cells were S-phase proliferating cells (BrdU+).
TS21: upper rhombic lip Present 3C The majority of positive cells were S-phase proliferating cells (BrdU+).
Specimen 3D: embryonic day 13.5; Tg(Nes-cre)1Atp/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant. Double labeled: red - Pax6; green - BrdU.
Structure Level Pattern Image Note
TS21: cerebellum layer Present Regionally restricted 3D Expressed in the cerebellum external granule cell layer. The majority of positive cells were S-phase proliferating cells (BrdU+).
TS21: upper rhombic lip Present 3D The majority of positive cells were S-phase proliferating cells (BrdU+).
Specimen 3L WT: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: cerebellum external granule cell layer Present 3L WT
TS24: upper rhombic lip Present 3L WT
Specimen 3L dCKO: embryonic day 16.5; Tg(Nes-cre)1Atp/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS24: cerebellum external granule cell layer Present 3L CKO Strikingly reduced number of positive cells compared with the wild-type.
TS24: upper rhombic lip Present 3L CKO Strikingly reduced number of positive cells compared with the wild-type.
Specimen 5A WT: postnatal day 0 (more )
Note: Double labeled: red - Pax6; green - Yap1 (assay MGI:7434448).
Structure Level Pattern Image Note
TS27: cerebellum external granule cell layer Present 5A WT left , 5A WT middle
Specimen 5A dCKO: postnatal day 0; Tg(Atoh1-cre)1Bfri/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant. Double labeled: red - Pax6; green - Yap1 (assay MGI:7434448).
Structure Level Pattern Image Note
TS27: cerebellum external granule cell layer Present 5A CKO left , 5A CKO middle
Specimen 5H left: postnatal day 7; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+, Tg(Atoh1-cre)1Bfri/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant. Double labeled: red - Pax6; green - Cre reporter.
Structure Level Pattern Image Note
TS28: cerebellum external granule cell layer Present 5H left
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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