About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:283394 Hughes LJ, et al., Yap/Taz are required for establishing the cerebellar radial glia scaffold and proper foliation. Dev Biol. 2020 Jan 1;457(1):150-162
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7434454
Gene symbol: Pax6
Gene name: paired box 6
Antibody: anti-Pax6 (#PRB-278P; #30130)
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 1B: embryonic day 16.5 (more )
Note: Double labeled: red - Pax6; green - Yap1 (assay MGI:7434448).
Structure Level Pattern Image Note
TS24: cerebellum external granule cell layer Present 1B right lower Overlay , 1B right lower Pax6 , 1B left , 1B right upper
Specimen 1D: postnatal day 0 (more )
Note: Double labeled: red - Pax6; green - Yap1 (assay MGI:7434448).
Structure Level Pattern Image Note
TS27: cerebellum external granule cell layer Present 1D middle , 1D left , 1D right Pax6 , 1D right Overlay
Specimen 3A: embryonic day 13.5 (more )
Note: Double labeled: red - Pax6; green - Yap1 (assay MGI:7434448).
Structure Level Pattern Image Note
TS21: cerebellum layer Present Regionally restricted 3A left , 3A middle upper Expressed in the cerebellum external granule cell layer.
TS21: upper rhombic lip Present 3A left , 3A right upper
Specimen 3B: embryonic day 13.5; Tg(Nes-cre)1Atp/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant. Double labeled: red - Pax6; green - Yap1 (assay MGI:7434448).
Structure Level Pattern Image Note
TS21: cerebellum layer Present Regionally restricted 3B left , 3B middle upper Expressed in the cerebellum external granule cell layer.
TS21: upper rhombic lip Present 3B left , 3B right upper Smaller size compared with the wild-type.
Specimen 3C: embryonic day 13.5 (more )
Note: Double labeled: red - Pax6; green - BrdU.
Structure Level Pattern Image Note
TS21: cerebellum layer Present Regionally restricted 3C Expressed in the cerebellum external granule cell layer. The majority of positive cells were S-phase proliferating cells (BrdU+).
TS21: upper rhombic lip Present 3C The majority of positive cells were S-phase proliferating cells (BrdU+).
Specimen 3D: embryonic day 13.5; Tg(Nes-cre)1Atp/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant. Double labeled: red - Pax6; green - BrdU.
Structure Level Pattern Image Note
TS21: cerebellum layer Present Regionally restricted 3D Expressed in the cerebellum external granule cell layer. The majority of positive cells were S-phase proliferating cells (BrdU+).
TS21: upper rhombic lip Present 3D The majority of positive cells were S-phase proliferating cells (BrdU+).
Specimen 3L WT: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: cerebellum external granule cell layer Present 3L WT
TS24: upper rhombic lip Present 3L WT
Specimen 3L dCKO: embryonic day 16.5; Tg(Nes-cre)1Atp/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS24: cerebellum external granule cell layer Present 3L CKO Strikingly reduced number of positive cells compared with the wild-type.
TS24: upper rhombic lip Present 3L CKO Strikingly reduced number of positive cells compared with the wild-type.
Specimen 5A WT: postnatal day 0 (more )
Note: Double labeled: red - Pax6; green - Yap1 (assay MGI:7434448).
Structure Level Pattern Image Note
TS27: cerebellum external granule cell layer Present 5A WT left , 5A WT middle
Specimen 5A dCKO: postnatal day 0; Tg(Atoh1-cre)1Bfri/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant. Double labeled: red - Pax6; green - Yap1 (assay MGI:7434448).
Structure Level Pattern Image Note
TS27: cerebellum external granule cell layer Present 5A CKO left , 5A CKO middle
Specimen 5H left: postnatal day 7; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+, Tg(Atoh1-cre)1Bfri/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant. Double labeled: red - Pax6; green - Cre reporter.
Structure Level Pattern Image Note
TS28: cerebellum external granule cell layer Present 5H left
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/21/2024
MGI 6.23 		The Jackson Laboratory