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Gene Expression Data
Assay Details
Assay
Reference: J:283394 Hughes LJ, et al., Yap/Taz are required for establishing the cerebellar radial glia scaffold and proper foliation. Dev Biol. 2020 Jan 1;457(1):150-162
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7434456
Gene symbol: S100b
Gene name: S100 protein, beta polypeptide, neural
Antibody: anti-S100b [EP1576Y] (ab52642, NB110-57478)
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 1E: postnatal day 5 (more )
Note: Double labeled: red - S100b; green - Yap1 (assay MGI:7434448).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Bergmann glial cell Present 1E right Overlay , 1E right S100b , 1E left
Specimen 7G WT: postnatal day 10 (more )
Note: Double labeled: red - S100b; green - Calb1 (assay MGI:7434575).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Bergmann glial cell Present 7G WT
Specimen 7G dCKO: postnatal day 10; Tg(Nes-cre)1Atp/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant. Double labeled: red - S100b; green - Calb1 (assay MGI:7434575).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Bergmann glial cell Present 7G CKO Smaller lobules II and III compared with the wild-type.
Specimen S1C: postnatal day 14; Tg(Nes-cre)1Atp/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant. Double labeled: red - S100b; green - Yap1 (assay MGI:7434448).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum external granule cell layer Bergmann glial cell Present S1C left , S1C middle
Specimen S5C: postnatal day 14 (more )
Structure Cell Type Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Bergmann glial cell Present S5C Cell bodies were closely aligned in the Purkinje cell layer, with processes that spaned the molecular layer and endfeet that form the glia limitans.
Specimen S5D: postnatal day 14; Tg(Nes-cre)1Atp/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Bergmann glial cell Present S5D Less tightly distributed, but still mainly located in the Purkinje cell layer.
Specimen S6A: postnatal day 8 (more )
Note: Double labeled: red - S100b; green - Yap1 (assay MGI:7434448).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Bergmann glial cell Present S6A
Specimen S6B: postnatal day 8; Tg(Gfap-cre)77.6Mvs/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant. Double labeled: red - S100b; green - Yap1 (assay MGI:7434448).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Bergmann glial cell Present S6B Similar as in the wild-type.
Specimen S6I: postnatal day 21; Tg(Gfap-cre)77.6Mvs/0, Wwtr1tm1.1Eno/Wwtr1+, Yap1tm1.1Eno/Yap1+, Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: red - S100b; green - Cre reporter.
Structure Cell Type Level Pattern Image Note
TS28: cerebellum vermis lobule IX Bergmann glial cell Present S6I
Specimen S6J: postnatal day 21; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor, Tg(Gfap-cre)77.6Mvs/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant. Double labeled: red - S100b; green - Cre reporter.
Structure Cell Type Level Pattern Image Note
TS28: cerebellum vermis lobule IX Bergmann glial cell Present S6J Similar as in the wild-type.
Specimen S6K: postnatal day 90; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor, Tg(Gfap-cre)77.6Mvs/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant. Double labeled: red - S100b; green - Cre reporter.
Structure Cell Type Level Pattern Image Note
TS28: cerebellum vermis lobule IX Bergmann glial cell Present S6K Similar as in the wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
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