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Gene Expression Data
Assay Details
Assay
Reference: J:290712 Ishiguro KI, et al., MEIOSIN Directs the Switch from Mitosis to Meiosis in Mammalian Germ Cells. Dev Cell. 2020 Feb 24;52(4):429-445.e10
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6721101
Gene symbol: Sycp3
Gene name: synaptonemal complex protein 3
Antibody: anti-SCP3 [Cor 10G11/7] (ab97672)
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Two Sycp3 antibodies were described, but the one used in this assay was not detailed. Anti-Sycp3 MGI:6720905 may have been used in this assay.
Results
Specimen S1F: postnatal day 18 (more )
Note: Multi-labeled: red - Sycp3 green - Meiosin (assay MGI:6721100); red - Stra8 (assay MGI:6721099); blue - DAPI
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Regionally restricted S1F Meiosin/Sycp3 , S1F Sycp3 Authors report Sycp3 is a component of meiotic axial element.
Specimen 1F left: postnatal week 8 (more )
Note: Triple labeled: red - Sycp3; green - Meiosin (assay MGI:6721100); blue - DAPI.
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 1F (left) Sycp3 , 1F (left) Authors report Sycp3 is a component of meiotic axial element.
Specimen 2H (p10) WT: postnatal day 10 (more )
Note: Triple labeled: red - Sycp3; green - Stra8 (assay MGI:6721099); blue - DAPI.
Structure Level Pattern Image Note
TS28: spermatocyte Present 2H (p10) WT
Specimen 2H (p10) Meiosin KO: postnatal day 10; Meiosinem1Keish/Meiosinem1Keish (more )
Note: Triple labeled: red - Sycp3; green - Stra8 (assay MGI:6721099); blue - DAPI.
Structure Level Pattern Image Note
TS28: spermatocyte Present 2H (p10) Meiosin KO
Specimen 2H (p10) Stra8 KO: postnatal day 10; Stra8em4Keish/Stra8em4Keish (more )
Note: Triple labeled: red - Sycp3; green - Stra8 (assay MGI:6721099); blue - DAPI.
Structure Level Pattern Image Note
TS28: spermatocyte Present 2H (p10) Stra8 KO
Specimen 2H (p18) WT: postnatal day 18 (more )
Note: Triple labeled: red - Sycp3; green - Stra8 (assay MGI:6721099); blue - DAPI.
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 2H (p18) WT First wave of spermatogenesis reached pachytene stage meiotic prophase in wild type.
Specimen 2H (P18) Meiosin KO: postnatal day 18; Meiosinem1Keish/Meiosinem1Keish (more )
Note: Triple labeled: red - Sycp3; green - Stra8 (assay MGI:6721099); blue - DAPI.
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 2H (p18) Meiosin KO Spermatocytes later than the preleptotene-like stage did not appear in Meiosin KO mice at postnatal day 18.
Specimen 2H (p18) Stra8 KO: postnatal day 18; Stra8em4Keish/Stra8em4Keish (more )
Note: Triple labeled: red - Sycp3; green - Stra8 (assay MGI:6721099); blue - DAPI.
Structure Level Pattern Image Note
TS28: spermatocyte Present 2H (p18) Stra8 KO
Specimen 2H (8w) WT: postnatal week 8 (more )
Note: Triple labeled: red - Sycp3; green - Stra8 (assay MGI:6721099); blue - DAPI.
Structure Level Pattern Image Note
TS28: spermatocyte Present 2H (8w) WT
Specimen 2H (8w) Meiosin KO: postnatal week 8; Meiosinem1Keish/Meiosinem1Keish (more )
Note: Triple labeled: red - Sycp3; green - Stra8 (assay MGI:6721099); blue - DAPI.
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 2H (8w) Meiosin KO Authors report that phenotype persisted through adulthood in Meiosin KO mice.
Specimen 2H (8w) Stra8 KO: postnatal week 8; Stra8em4Keish/Stra8em4Keish (more )
Note: Triple labeled: red - Sycp3; green - Stra8 (assay MGI:6721099); blue - DAPI.
Structure Level Pattern Image Note
TS28: spermatocyte Present 2H (p18) Stra8 KO
Specimen 3A (p10) WT: postnatal day 10 (more )
Note: Triple labeled: red - Sycp3; green - H3S10P; blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 3A (p10) WT
Specimen 3A (p10) Meiosin KO: postnatal day 10; Meiosinem1Keish/Meiosinem1Keish (more )
Note: Triple labeled: red - Sycp3; green - H3S10P; blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 3A (p10) Meiosin KO
Specimen 3A (p10) Stra8 KO: postnatal day 10; Stra8em4Keish/Stra8em4Keish (more )
Note: Triple labeled: red - Sycp3; green - H3S10P; blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 3A (p10) Stra8 KO
Specimen 3A (p18) WT: postnatal day 18 (more )
Note: Triple labeled: red - Sycp3; green - H3S10P; blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 3A (p18) WT
Specimen 3A (p18) Meiosin KO: postnatal day 18; Meiosinem1Keish/Meiosinem1Keish (more )
Note: Triple labeled: red - Sycp3; green - H3S10P; blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 3A (p18) Meiosin KO
Specimen 3A (p18) Stra8 KO: postnatal day 18; Stra8em4Keish/Stra8em4Keish (more )
Note: Triple labeled: red - Sycp3; green - H3S10P; blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 3A (p18) Stra8 KO
Specimen 3A (8w) WT: postnatal week 8 (more )
Note: Triple labeled: red - Sycp3; green - H3S10P; blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 3A (8w) WT
Specimen 3A (8w) Meiosin KO: postnatal week 8; Meiosinem1Keish/Meiosinem1Keish (more )
Note: Triple labeled: red - Sycp3; green - H3S10P; blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 3A (8w) Meiosin KO
Specimen 3A (8w) Stra8 KO: postnatal week 8; Stra8em4Keish/Stra8em4Keish (more )
Note: Triple labeled: red - Sycp3; green - H3S10P; blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 3A (8w) Stra8 KO
Specimen 3B: postnatal week 4; Meiosinem1Keish/Meiosinem1Keish (more )
Note: Triple labeled: red - Sycp3; green - H3S10P; blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 3B (merge) , 3B Sycp3
Specimen 3D WT: postnatal day 18 (more )
Note: Multi-labeled: white - Sycp3; red - EdU; green - H3S10P; blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 3D WT Sycp3
Specimen 3D Meiosin KO: postnatal day 18; Meiosinem1Keish/Meiosinem1Keish (more )
Note: Multi-labeled: white - Sycp3; red - EdU; green - H3S10P; blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 3D Meiosin KO Sycp3
Specimen 3D Stra8 KO: postnatal day 18; Stra8em4Keish/Stra8em4Keish (more )
Note: Multi-labeled: white - Sycp3; red - EdU; green - H3S10P; blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 3D Stra8 KO Sycp3
Specimen 3F WT: postnatal day 18 (more )
Note: Triple labeled: red - Sycp3; green - Ccna2 (assay MGI:6721109); blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 3F WT (merge)
Specimen 3F Meiosin KO: postnatal day 18; Meiosinem1Keish/Meiosinem1Keish (more )
Note: Triple labeled: red - Sycp3; green - Ccna2 (assay MGI:6721109); blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 3F Meiosin KO (merge)
Specimen 3G WT: postnatal week 8 (more )
Note: Triple labeled: red - Sycp3; green - TUNEL; blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 3G WT (merge)
Specimen 3G Meiosin KO: postnatal week 8; Meiosinem1Keish/Meiosinem1Keish (more )
Note: Triple labeled: red - Sycp3; green - TUNEL; blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 3G Meiosin KO (merge)
Specimen 4A WT: postnatal week 8 (more )
Note: Triple labeled: red - Sycp3; green - H2ax (assay MGI:6721110); blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 4A WT (merge) , 4A WT Sycp3
Specimen 4A Meiosin KO: postnatal week 8; Meiosinem1Keish/Meiosinem1Keish (more )
Note: Triple labeled: red - Sycp3; green - H2ax (assay MGI:6721110); blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 4A Meiosin KO (merge) , 4A Meiosin KO Sycp3
Specimen 4A Stra8 KO: postnatal week 8; Stra8em4Keish/Stra8em4Keish (more )
Note: Triple labeled: red - Sycp3; green - H2ax (assay MGI:6721110); blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 4A Stra8 KO Sycp3 , 4A Stra8 KO (merge)
Specimen 4B WT: postnatal week 8 (more )
Note: Triple labeled: red - Sycp3; green - Dmc1 (assay MGI:6721111); blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 4B WT Sycp3 , 4B WT (merge)
Specimen 4B Meiosin KO: postnatal week 8; Meiosinem1Keish/Meiosinem1Keish (more )
Note: Triple labeled: red - Sycp3; green - Dmc1 (assay MGI:6721111); blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 4B Meiosin KO Sycp3 , 4B Meiosin KO (merge)
Specimen 4B Stra8 KO: postnatal week 8; Stra8em4Keish/Stra8em4Keish (more )
Note: Triple labeled: red - Sycp3; green - Dmc1 (assay MGI:6721111); blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 4B Stra8 KO Sycp3 , 4B Stra8 KO (merge)
Specimen 4D WT: postnatal week 8 (more )
Note: Triple labeled: red - Sycp3; green - Sycp1 (assay MGI:6721112); blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 4D WT Sycp3 , 4D WT (merge)
Specimen 4D Meiosin KO: postnatal week 8; Meiosinem1Keish/Meiosinem1Keish (more )
Note: Triple labeled: red - Sycp3; green - Sycp1 (assay MGI:6721112); blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 4D Meiosin KO Sycp3 , 4D Meiosin KO (merge)
Specimen 4D Stra8 KO: postnatal week 8; Stra8em4Keish/Stra8em4Keish (more )
Note: Triple labeled: red - Sycp3; green - Sycp1 (assay MGI:6721112); blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 4D Stra8 KO Sycp3 , 4D Stra8 KO (merge)
Specimen 7B E15.5 WT: embryonic day 15.5 (more )
Note: Triple labeled: red - Sycp3; green - Sycp1 (assay MGI:6721112); blue - DAPI.
Structure Level Pattern Image Note
TS23: germ cell of ovary Present 7B E15.5 WT S3 , 7B E15.5 WT (merge)
Specimen 7B E15.5 Meiosin KO: embryonic day 15.5; Meiosinem1Keish/Meiosinem1Keish (more )
Note: Triple labeled: red - Sycp3; green - Sycp1 (assay MGI:6721112); blue - DAPI.
Structure Level Pattern Image Note
TS23: germ cell of ovary Present Patchy 7B E15.5 Meiosin KO (merge) , 7B E15.5 Meiosin KO S3 Patchy aggregates of expression.
Specimen 7B E17.5 WT: embryonic day 17.5 (more )
Note: Triple labeled: red - Sycp3; green - Sycp1 (assay MGI:6721112); blue - DAPI.
Structure Level Pattern Image Note
TS25: germ cell of ovary Present 7B E17.5 WT S3 , 7B E17.5 WT (merge)
Specimen 7B E17.5 Meiosin KO: embryonic day 17.5; Meiosinem1Keish/Meiosinem1Keish (more )
Note: Triple labeled: red - Sycp3; green - Sycp1 (assay MGI:6721112); blue - DAPI.
Structure Level Pattern Image Note
TS25: germ cell of ovary Present Patchy 7B E17.5 Meiosin KO (merge) , 7B E17.5 Meiosin KO S3 Patchy aggregates of expression.
Specimen 7C WT: embryonic day 17.5 (more )
Note: Triple labeled: red - Sycp3; green - H3S10P; blue - DAPI.
Structure Level Pattern Image Note
TS25: germ cell of ovary Present 7C WT (merge) , 7C WT Sycp3
Specimen 7C Meiosin KO: embryonic day 17.5; Meiosinem1Keish/Meiosinem1Keish (more )
Note: Triple labeled: red - Sycp3; green - H3S10P; blue - DAPI.
Structure Level Pattern Image Note
TS25: germ cell of ovary Present Patchy 7C Meiosin KO (merge) , 7C Meiosin KO Sycp3 Patchy aggregates of expression.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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