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Gene Expression Data
Assay Details
Assay
Reference: J:290712 Ishiguro KI, et al., MEIOSIN Directs the Switch from Mitosis to Meiosis in Mammalian Germ Cells. Dev Cell. 2020 Feb 24;52(4):429-445.e10
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6721110
Gene symbol: H2ax
Gene name: H2A.X variant histone
Antibody: anti-gamma H2A.X (phospho S139) (abcam; ab11174)
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Two H2ax antibodies were described, but the one used in this assay was not detailed. anti-gamma H2A.X (phospho S139) [9F3] (ab26350) MGI:6720909 may have been used in this assay.
Results
Specimen 4A WT: postnatal week 8 (more )
Note: Triple labeled: green - H2ax; red - Sycp3 (assay MGI:6721101); blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 4A WT (merge) , 4A WT H2ax
Specimen 4A Meiosin KO: postnatal day 8; Meiosinem1Keish/Meiosinem1Keish (more )
Note: Triple labeled: green - H2ax; red - Sycp3 (assay MGI:6721101); blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 4A Meiosin KO (merge) , 4A Meiosin KO H2ax Expression detected in Meiosin KO.
Specimen 4A Stra8 KO: postnatal day 8; Stra8em4Keish/Stra8em4Keish (more )
Note: Triple labeled: green - H2ax; red - Sycp3 (assay MGI:6721101); blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 4A Stra8 KO (merge) , 4A Stra8 KO H2ax
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
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