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Gene Expression Data
Assay Details
Assay
Reference: J:290712 Ishiguro KI, et al., MEIOSIN Directs the Switch from Mitosis to Meiosis in Mammalian Germ Cells. Dev Cell. 2020 Feb 24;52(4):429-445.e10
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6721099
Gene symbol: Stra8
Gene name: stimulated by retinoic acid gene 8
Antibody: Stra8 antibody
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen S1A: postnatal adult; Stra8em1Keish/Stra8em1Keish (more )
Note: Triple labeled: red - Stra8 protein; green - Stra8 reporter (assay MGI:6721082); blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present S1A (merge) , S1A Stra8
Specimen S1B: postnatal adult; Stra8em1Keish/Stra8em1Keish (more )
Note: Triple labeled: red - Stra8; green - HA; blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present S1B (merge) , S1B Stra8
Specimen S1F: postnatal day 18 (more )
Note: Multi-labeled: red - Stra8; green - Meiosin (assay MGI:6721100); red - Sycp3 (assay MGI:6721101); blue - DAPI.
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted S1F Stra8 , S1F Meiosin/Stra8 Expression in cytoplasm and nuclei of preleptotene spermatocytes in stage VII-VIII seminiferous tubules.
Specimen 1F (left center): postnatal week 8 (more )
Note: Triple labeled: red - Stra8; green - Meiosin (assay MGI:6721100); blue - DAPI.
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 1F (left center) , 1F (left center) Stra8 Expression in cytoplasm and nuclei of preleptotene spermatocytes in stage VII-VIII seminiferous tubules.
TS28: spermatid Absent 1F (left center) , 1F (left center) Stra8
Specimen 1G: postnatal adult (more )
Note: Triple labeled: red - Stra8; green - Meiosin (assay MGI:6721100); blue - Tra98 (Gcna assay MGI:6721105).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Regionally restricted 1G Stra8 , 1G (merge) Co-expression with Meiosin in a limited area along the tubules.
Specimen 1H E13.5: embryonic day 13.5 (more )
Note: Triple labeled: red - Stra8; green - Meiosin (assay MGI:6721100); blue - Tra98 (Gcna assay MGI:6721105).
Structure Level Pattern Image Note
TS21: germ cell of ovary Present 1H E13.5 Stra8 , 1H E13.5 (merge)
Specimen 1H E14.5: embryonic day 14.5 (more )
Note: Triple labeled: red - Stra8; green - Meiosin (assay MGI:6721100); blue - Tra98 (Gcna assay MGI:6721105).
Structure Level Pattern Image Note
TS22: germ cell of ovary Present 1H E14.5 Stra8 , 1H E14.5 (merge)
Specimen 1H E15.5: embryonic day 15.5 (more )
Note: Triple labeled: red - Stra8; green - Meiosin (assay MGI:6721100); blue - Tra98 (Gcna assay MGI:6721105).
Structure Level Pattern Image Note
TS23: germ cell of ovary Weak 1H E15.5 Stra8 , 1H E15.5 (merge) Gradual reduction at E15.5.
Specimen 2G WT: postnatal day 18 (more )
Note: Triple labeled: red - Stra8; green - Meiosin (assay MGI:6721100); blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Regionally restricted 2G WT Expression mainly in nucleus.
Specimen 2G Meiosin KO: postnatal day 18; Meiosinem1Keish/Meiosinem1Keish (more )
Note: Triple labeled: red - Stra8; green - Meiosin (assay MGI:6721100); blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Regionally restricted 2G Meiosin KO Expression mainly in cytoplasm.
Specimen 2G Stra8 KO: postnatal day 18; Stra8em4Keish/Stra8em4Keish (more )
Note: Triple labeled: red - Stra8; green - Meiosin (assay MGI:6721100); blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Absent 2G Stra8 KO
Specimen 2H (p10) WT: postnatal day 10 (more )
Note: Triple labeled: green - Stra8; red - Sycp3 (assay MGI:6721101); blue - DAPI.
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 2H (p10) WT Expression mainly in nucleus.
Specimen 2H (p10) Meiosin KO: postnatal day 10; Meiosinem1Keish/Meiosinem1Keish (more )
Note: Triple labeled: green - Stra8; red - Sycp3 (assay MGI:6721101); blue - DAPI.
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 2H (p10) Meiosin KO Expression mainly in cytoplasm.
Specimen 1H (p10) Stra8 KO: postnatal day 10; Stra8em4Keish/Stra8em4Keish (more )
Note: Triple labeled: green - Stra8; red - Sycp3 (assay MGI:6721101); blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Absent 2H (p10) Stra8 KO
Specimen 2H (p18) WT: postnatal day 18 (more )
Note: Triple labeled: green - Stra8; red - Sycp3 (assay MGI:6721101); blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Regionally restricted 2H (p18) WT Expression mainly in nucleus.
Specimen 2H (p18) Meiosin KO: postnatal day 18; Meiosinem1Keish/Meiosinem1Keish (more )
Note: Triple labeled: green - Stra8; red - Sycp3 (assay MGI:6721101); blue - DAPI.
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 2H (p18) Meiosin KO Expression mainly in cytoplasm.
Specimen 2H (p18) Stra8 KO: postnatal day 18; Stra8em4Keish/Stra8em4Keish (more )
Note: Triple labeled: green - Stra8; red - Sycp3 (assay MGI:6721101); blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Absent 2H (p18) Stra8 KO
Specimen 2H (8w) WT: postnatal week 8 (more )
Note: Triple labeled: green - Stra8; red - Sycp3 (assay MGI:6721101); blue - DAPI.
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 2H (8w) WT
Specimen 2H (8w) Meiosin KO: postnatal week 8; Meiosinem1Keish/Meiosinem1Keish (more )
Note: Triple labeled: green - Stra8; red - Sycp3 (assay MGI:6721101); blue - DAPI.
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 2H (8w) Meiosin KO Expression mainly in cytoplasm.
Specimen 2H (8w) Stra8 KO: postnatal week 8; Stra8em4Keish/Stra8em4Keish (more )
Note: Triple labeled: green - Stra8; red - Sycp3 (assay MGI:6721101); blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Absent 2H (8w) Stra8 KO
Specimen 2K: postnatal week 8; Meiosinem1Keish/Meiosinem1Keish (more )
Note: Triple labeled: green - Stra8; red - c-kit (Kit assay MGI:6721106); blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 2K (merge) , 2K Stra8
Specimen 2L WT: postnatal week 8 (more )
Note: Triple labeled: green - Stra8; red - EdU; blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 2L WT
Specimen 2L Meiosin KO: postnatal week 8; Meiosinem1Keish/Meiosinem1Keish (more )
Note: Triple labeled: green - Stra8; red - EdU; blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 2L Meiosin KO
Specimen 2L Stra8 KO: postnatal week 8; Stra8em4Keish/Stra8em4Keish (more )
Note: Triple labeled: green - Stra8; red - EdU; blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Absent 2L Stra8 KO
Specimen 7A WT: embryonic day 13.5 (more )
Note: Triple labeled: green - Stra8; red - Meiosin (assay MGI:6721100); blue - DAPI.
Structure Level Pattern Image Note
TS21: germ cell of ovary Present 7A WT (merge) , 7A WT Stra8
Specimen 7A Meiosin KO: embryonic day 13.5; Meiosinem1Keish/Meiosinem1Keish (more )
Note: Triple labeled: green - Stra8; red - Meiosin (assay MGI:6721100); blue - DAPI.
Structure Level Pattern Image Note
TS21: germ cell of ovary Present 7A Meiosin KO (merge) , 7A Meiosin KO Stra8
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
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