Reporter:	lacZ
Antibody:	anti-B-galactosidase (ab9361)
Detection system:	Secondary antibody coupled to Alexa Fluor
Assay notes:	The authors reported using multiple B-gal antibodies but did not specify which was used to generate these data; it is possible MGI:6402319 was used.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	2K , 2I	Irx6:beta-gal positive cells were co-labeled with Vsx2 immunopositive cells in a subset of bipolar cells.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	2L , 2N	Irx6:beta-gal positive cells co-labeled with Irx5 immunopositive cells in a subset of bipolar cells. Not all Irx5-positive cells co-labeled with Irx6:beta-gal, and not all Irx6:beta-gal positive cells co-labeled with Irx5.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	3B , 3C	Irx6:beta-gal positive cells co-labeled with Tacr3 immunopositive cells in a subset of bipolar cells. The subset co-labeled was type 2 bipolar cells.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	3F , 3E	Irx6:beta-gal positive cells co-labeled with Rcvrn immunopositive cells in a subset of bipolar cells. The subset co-labeled was type 2 bipolar cells.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	3H , 3I	Irx6:beta-gal positive cells co-labeled with Cabp5 immunopositive cells in a subset of bipolar cells. The subset co-labeled was type 3a bipolar cells.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	3K , 3L	Irx6:beta-gal positive cells co-labeled with Hcn4 immunopositive cells in a subset of bipolar cells. The subset co-labeled was type 3a bipolar cells.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	3N , 3O	Irx6:beta-gal positive bipolar cells did not co-label with Prkar2b immunopositive type 3b bipolar cells.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	3Q , 3R	Irx6:beta-gal positive bipolar cells did not co-label with Prkca immunopositive rod bipolar cells.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	4C , 4A	Expression was detected in a subset of Vsx2-positive bipolar cells.
TS28: retina ganglion cell layer	Present		4C , 4A
TS28: retina layer	Present	Regionally restricted	4A , 4C	In addition to the presence of staining in the OFF sublamina of the inner plexiform layer, expression was also detected in the ON sublamina.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	4F , 4D	Similar to the Irx6<+/lacZ> retina, labeling exhibited overlapping and non-overlapping expression with Irx5 in the mutant.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	6D , 6A	Type 3a bipolar cells were shown to be triple labeled by Cabp5, Hcn4, and Irx6:b-gal.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	6J , 6G	In the mutant, Irx6:b-gal and Hcn4 expression continued, but Cabp5 expression was reduced in type 3a cells.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	6F	Type 3a bipolar cells were shown to be double labeled by Cabp5 and Irx6:b-gal.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	6L	In the mutant, Irx6:b-gal expression continued, but Cabp5 expression was reduced in type 3a cells.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present		S2A'' , S2A'	The Irx6:beta-gal reporter co-immunolabeled with the ganglion cell marker Pou4f2 in a subset of cells in the adult retina.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	S2B' , S2B''	Irx6:beta-gal positive bipolar cells did not co-label with Kcnip3 immunopositive type 4 bipolar cells.

Structure	Level	Pattern	Image	Note
TS28: retina inner plexiform layer	Present	Regionally restricted	S3C , S3A	Irx6:beta-gal co-labeled with Syt2 immunolabeled axons in the upper and lower zones of the inner plexiform layer corresponding to the OFF and ON projecting region.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present			There was a significant increase in the number of Irx6:b-gal cells in the absence of Vsx1.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	S4_merge Irx6LacZ/LacZ;Vsx1+/AltB5 , S4_Beta-gal Irx6LacZ/LacZ;Vsx1+/AltB5	Expression was detected in cells that display chracteristics of both type 2 and 3a bipolar cells. Not all Irx6-bgal-positive cells co-immunolabeled Hcn4. Bhlhe22 immunolabeling is present in some of the Irx6:bgal-positive cells that do not co-immunolabel Hcn4.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	S4_merge Irx6LacZ/LacZ;Vsx1AltB5/AltB5 , S4_Beta-gal Irx6LacZ/LacZ;Vsx1AltB5/AltB5	Expression was detected in cells that display chracteristics of both type 2 and 3a bipolar cells. Not all Irx6-bgal-positive cells co-immunolabeled Hcn4. Bhlhe22 immunolabeling is present in some of the Irx6:bgal-positive cells that do not co-immunolabel Hcn4.