Gene Expression Data
Assay Details
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Assay
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Reference:
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J:189955
Star EN, et al., Regulation of retinal interneuron subtype identity by the Iroquois homeobox gene Irx6. Development. 2012 Dec;139(24):4644-55
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6450184
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Gene symbol:
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Cabp5
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Gene name:
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calcium binding protein 5
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Antibody:
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Anti-Cabp5
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Results
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Specimen
3G:
postnatal month 2-3; Irx6tm1Chow/Irx6 +
(more  )
Note: Double labeled: red - Cabp5; green - Irx6 (assay MGI:6450171).
Specimen
3G:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129S1/SvImj * 129X1/SvJ
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Mutant Allele(s):
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Irx6tm1Chow/Irx6+
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Age:
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postnatal month 2-3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Cabp5; green - Irx6 (assay MGI:6450171).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina inner nuclear layer |
Present |
Regionally restricted |
3I
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3G
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Cabp5 labels type 3a, 3b, 5, and rod bipolar cells. Cabp5 co-labeled with Irx6:b-gal positive cells in a subset of bipolar cells.
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Specimen
5G:
postnatal month 2-3; Irx6tm1Chow/Irx6 +
(more )
Specimen
5G:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S1/SvImj * 129X1/SvJ
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Mutant Allele(s):
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Irx6tm1Chow/Irx6+
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Age:
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postnatal month 2-3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina inner plexiform layer |
Present |
Regionally restricted |
5G
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Expression was detected in the region of the inner plexiform layer occupied by the axon terminal of type 3 and 5 and rod bipolar cells.
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Specimen
5H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S1/SvImj * 129X1/SvJ
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Mutant Allele(s):
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Irx6tm1Chow/Irx6tm1Chow
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Age:
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postnatal month 2-3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina inner plexiform layer |
Present |
Regionally restricted |
5H
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Expression was reduced in the region of the inner plexiform layer occupied by the axon terminal of type 3 cells. Expression was unaffected in the regions of the axon termini of type 5 and rod bipolar cells.
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Specimen
6C/D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S1/SvImj * 129X1/SvJ
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Mutant Allele(s):
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Irx6tm1Chow/Irx6+
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Age:
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postnatal month 2-3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: blue - Cabp5; green - Irx6 (assay MGI:6450171); red - Hcn4 (assay MGI:6450186).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina inner nuclear layer |
Present |
Regionally restricted |
6C
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6D
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Type 3a bipolar cells were shown to be triple labeled by Cabp5, Hcn4, and Irx6:b-gal.
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Specimen
6I/J:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S1/SvImj * 129X1/SvJ
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Mutant Allele(s):
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Irx6tm1Chow/Irx6tm1Chow
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Age:
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postnatal month 2-3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: blue - Cabp5; green - Irx6 (assay MGI:6450171); red - Hcn4 (assay MGI:6450186).
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Specimen
6E/F:
postnatal month 2-3; Irx6tm1Chow/Irx6 +
(more )
Note: Triple labeled: blue - Cabp5; green - Irx6 (assay MGI:6450171); red - Prkar2b (assay MGI:6450187).
Specimen
6E/F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S1/SvImj * 129X1/SvJ
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Mutant Allele(s):
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Irx6tm1Chow/Irx6+
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Age:
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postnatal month 2-3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: blue - Cabp5; green - Irx6 (assay MGI:6450171); red - Prkar2b (assay MGI:6450187).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina inner nuclear layer |
Present |
Regionally restricted |
6F
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6E
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Cabp5 was detected in Type 3a and 3b bipolar cells. Expression co-localized with Irx6:b-gal in type 3a cells and with Prkar2b in type 3b cells.
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Specimen
6K/L:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S1/SvImj * 129X1/SvJ
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Mutant Allele(s):
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Irx6tm1Chow/Irx6tm1Chow
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Age:
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postnatal month 2-3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: blue - Cabp5; green - Irx6 (assay MGI:6450171); red - Prkar2b (assay MGI:6450187).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina inner nuclear layer |
Weak |
Regionally restricted |
6L
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6K
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In the mutant, Cabp5 expression was reduced in type 3a cells, but there was no change in type 3b cells.
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Analysis Tools