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Dhfr Gene Detail
Summary
  • Symbol
    Dhfr
  • Name
    dihydrofolate reductase
  • Synonyms
    8430436I03Rik
  • Feature Type
    protein coding gene
  • IDs
    MGI:94890
    NCBI Gene: 13361
  • Alliance
    gene page
  • Transcription Start Sites
    5 TSS
Location &
Maps
more
  • Sequence Map
    Chr13:92491291-92525561 bp, + strand
    From NCBI annotation of GRCm39
  • View this region in JBrowse
  • Genome Browsers
    Ensembl | UCSC | NCBI
  • Genetic Map
    Chromosome 13, 47.64 cM
  • Mapping Data
    44 experiments
Strain
Comparison
more
  • SNPs within 2kb
    161 from dbSNP Build 142
  • Strain Annotations
    18
  • PCR
    1
  • RFLP
    14
For selected strains:
Strain Gene Model ID Feature Type Coordinates Select Strains
C57BL/6J MGI_C57BL6J_94890
 protein coding gene Chr13:92491234-92525561 (+)
129S1/SvImJ MGP_129S1SvImJ_G0020814
 protein coding gene Chr13:93651509-93686453 (+)
A/J MGP_AJ_G0020768
 protein coding gene Chr13:90194564-90229588 (+)
AKR/J MGP_AKRJ_G0020741
 protein coding gene Chr13:92916704-92950617 (+)
BALB/cJ MGP_BALBcJ_G0020764
 protein coding gene Chr13:90831525-90865794 (+)
C3H/HeJ MGP_C3HHeJ_G0020558
 protein coding gene Chr13:92615151-92648818 (+)
C57BL/6NJ MGP_C57BL6NJ_G0021201
 protein coding gene Chr13:96505418-96539460 (+)
CAROLI/EiJ MGP_CAROLIEiJ_G0018768
 protein coding gene Chr13:86111224-86144143 (+)
CAST/EiJ MGP_CASTEiJ_G0020077
 protein coding gene Chr13:91897150-91925423 (+)
CBA/J MGP_CBAJ_G0020517
 protein coding gene Chr13:100344329-100381226 (+)
DBA/2J MGP_DBA2J_G0020642
 protein coding gene Chr13:89945674-89979586 (+)
FVB/NJ MGP_FVBNJ_G0020622
 protein coding gene Chr13:88772228-88806045 (+)
LP/J MGP_LPJ_G0020715
 protein coding gene Chr13:93773356-93807760 (+)
NOD/ShiLtJ MGP_NODShiLtJ_G0020651
 protein coding gene Chr13:100843968-100879185 (+)
NZO/HlLtJ MGP_NZOHlLtJ_G0021241
 protein coding gene Chr13:92326667-92363989 (+)
PWK/PhJ MGP_PWKPhJ_G0019829
 protein coding gene Chr13:88697823-88732648 (+)
SPRET/EiJ MGP_SPRETEiJ_G0019653
 protein coding gene Chr13:88585961-88622944 (+)
WSB/EiJ MGP_WSBEiJ_G0020134
 protein coding gene Chr13:92805033-92840430 (+)



Homology
more
  • Human Ortholog
    DHFR, dihydrofolate reductase
  • Vertebrate Orthologs
    5
Vertebrate Orthology Source
Alliance of Genome Resources
  • Human Ortholog
    DHFR, dihydrofolate reductase
  • Synonyms
    DHFR1, DHFRP1, DYR
  • Links
    NCBI Gene ID: 1719
    neXtProt AC: NX_P00374
    UniProt: P00374

  • Chr Location
    5q14.1; chr5:80626226-80654983 (-)  GRCh38
  • Human Ortholog
    DHFR2, dihydrofolate reductase 2
  • Synonyms
    DHFRL1, DHFRP4
  • Links
    NCBI Gene ID: 200895
    neXtProt AC: NX_Q86XF0
    UniProt: Q86XF0

  • Chr Location
    3q11.2; chr3:94047836-94063389 (-)  GRCh38
  • Human Ortholog
    DHFRP1, dihydrofolate reductase pseudogene 1
  • Links
    NCBI Gene ID: 573971
    UniProt: P00374

  • Chr Location
    18q11.2; chr18:26167862-26171357 (-)  GRCh38
Human Diseases
more
  • Diseases
    1 with human DHFR associations

Human Disease Mouse Models
      
IDs
Click on a disease name to see all genes associated with that disease.

Mutations,
Alleles, and
Phenotypes
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  • Phenotype Summary
    24 phenotypes from 2 alleles in 2 genetic backgrounds
    19 phenotype references
Phenotype Overview

adipose tissue
behavior/neurological
cardiovascular system
cellular
craniofacial
digestive/alimentary system
embryo
endocrine/exocrine glands
growth/size/body
hearing/vestibular/ear
hematopoietic system
homeostasis/metabolism
integument
immune system
limbs/digits/tail
liver/biliary system
mortality/aging
muscle
nervous system
pigmentation
renal/urinary system
reproductive system
respiratory system
skeleton
taste/olfaction
neoplasm
vision/eye

Click cells to view annotations.
Mice homozygous for a knock-out allele exhibit lethality soon after E14.5 with reduced size, pallor, impaired hematopoiesis and abnormal folic acid levels. Mice heterozygous for this allele exhibit abnormal folic acid levels, abnormal hemopoiesis and reduced endothelium-dependent vasorelaxation.
Gene Ontology
(GO)
Classifications
less
  • All GO Annotations
    42
  • GO References
    13
Molecular Function

carbohydrate derivative binding
cytoskeletal protein binding
DNA binding
enzyme regulator
hydrolase
ligase
lipid binding
oxidoreductase
RNA binding
signaling receptor activity
signaling receptor binding
transcription
transferase
transporter
Biological Process

carbohydrate derivative metabolism
cell differentiation
cell population proliferation
cellular component organization
DNA-templated transcription
establishment of localization
homeostatic process
immune system process
lipid metabolic process
programmed cell death
protein metabolic process
response to stimulus
signaling
system development
Cellular Component

cell projection
cytoplasmic vesicle
cytoskeleton
cytosol
endoplasmic reticulum
endosome
extracellular region
Golgi apparatus
mitochondrion
non-membrane-bounded organelle
nucleus
organelle envelope
organelle lumen
plasma membrane
protein-containing complex
synapse
vacuole
Click cells to view annotations.
Expression
less
Expression Overview

early conceptus
embryo ectoderm
embryo endoderm
embryo mesoderm
embryo mesenchyme
extraembryonic component
alimentary system
auditory system
branchial arches
cardiovascular system
connective tissue
endocrine system
exocrine system
hemolymphoid system
integumental system
limbs
liver and biliary system
musculoskeletal system
nervous system
olfactory system
reproductive system
respiratory system
urinary system
visual system
Click cells to view annotations.


  • Assay Results
    837
  • Tissues
    117
  • cDNA Data
    70
  • Literature Summary
    12
  • Comparison Matrix
    Gene Expression + Phenotype
    • Sequences &
    Gene Models
    less
    Representative SequencesLengthStrain/SpeciesFlank
    genomic 13361 NCBI Gene Model | MGI Sequence Detail 34271 C57BL/6J ±  kb
    transcript NM_010049 RefSeq | MGI Sequence Detail 5307 ZRU/MplStud  
    polypeptide P00375 UniProt | EBI | MGI Sequence Detail 187 Not Applicable  
    For the selected sequence
    Protein
    Information
    less
    Molecular
    Reagents
    less
    • All nucleic 80
      Genomic 3
      cDNA 71
      Primer pair 3
      Other 3

      Microarray probesets 6
    Other
    Accession IDs
    less
    MGD-MRK-8841, MGI:1918808, MGI:2145281, MGI:2145455, MGI:3034358
    References
    more
    • Summaries
      All 121
      Developmental Gene Expression 12
      Gene Ontology 13
      Phenotypes 19
    • Earliest
      J:30524 HAKALA MT, et al., Relation of folic acid reductase to amethopterin resistance in cultured mammalian cells. J Biol Chem. 1961 Mar;236:952-8
    • Latest
      J:341708 Saha S, et al., Dihydrofolate reductase activity controls neurogenic transitions in the developing neocortex. Development. 2023 Oct 15;150(20):dev201696
    Contributing Projects:
    Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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    Send questions and comments to User Support.     		last database update
    04/16/2024
    MGI 6.23     		The Jackson Laboratory