About   Help   FAQ
Dhfr Gene Detail
Summary
  • Symbol
    Dhfr
  • Name
    dihydrofolate reductase
  • Synonyms
    8430436I03Rik
  • Feature Type
    protein coding gene
  • IDs
    MGI:94890
    NCBI Gene: 13361
  • Alliance
    genome feature page
  • Transcription Start Sites
    5 TSS
Location &
Maps
more
  • Sequence Map
    Chr13:92491291-92525561 bp, + strand
    From NCBI annotation of GRCm39
  • View this region in JBrowse
  • Genome Browsers
    Ensembl | UCSC | NCBI
  • Genetic Map
    Chromosome 13, 47.64 cM
  • Mapping Data
    44 experiments
Strain
Comparison
more
  • SNPs within 2kb
    1002 from dbSNP Build 142
  • Strain Annotations
    19
  • PCR
    1
  • RFLP
    14
For selected strains:
Strain Gene Model ID Feature Type Coordinates Select Strains
C57BL/6J MGI_C57BL6J_94890
 protein coding gene Chr13:92491234-92525561 (+)
129S1/SvImJ ENSMUSG00200008855
 protein coding gene Chr13:86987893-87022243 (+)
A/J ENSMUSG00195023932
 protein coding gene Chr13:87500446-87534627 (+)
AKR/J ENSMUSG00220013731
 protein coding gene Chr13:85983210-86017544 (+)
BALB/cJ ENSMUSG00180015490
 protein coding gene Chr13:88024129-88058447 (+)
C3H/HeJ ENSMUSG00175023206
 protein coding gene Chr13:88202736-88237061 (+)
C57BL/6NJ ENSMUSG00215002956
 protein coding gene Chr13:88088336-88122666 (+)
CAROLI/EiJ MGP_CAROLIEiJ_G0018768
 protein coding gene Chr13:86111224-86144143 (+)
CAST/EiJ ENSTCUG00005009047
 protein coding gene Chr13:87321892-87349369 (+)
CBA/J ENSMUSG00210024301
 protein coding gene Chr13:87484489-87518832 (+)
DBA/2J ENSMUSG00185017355
 protein coding gene Chr13:91170530-91204873 (+)
FVB/NJ ENSMUSG00205007940
 protein coding gene Chr13:86975639-87009983 (+)
JF1/MsJ ENSUMUG00000019738
 protein coding gene Chr13:90911968-90946061 (+)
LP/J ENSMUSG00230034077
 protein coding gene Chr13:101188638-101222980 (+)
NOD/ShiLtJ ENSMUSG00190017596
 protein coding gene Chr13:87870812-87905140 (+)
NZO/HlLtJ ENSMUSG00225032429
 protein coding gene Chr13:93716950-93751278 (+)
PWK/PhJ ENSLUMG00010011187
 protein coding gene Chr13:87015859-87049999 (+)
SPRET/EiJ ENSMSPG00010006491
 protein coding gene Chr13:88217278-88253578 (+)
WSB/EiJ ENSIUOG00005029962
 protein coding gene Chr13:86893103-86927446 (+)



Homology
more
  • Human Ortholog
    DHFR, dihydrofolate reductase
  • Vertebrate Orthologs
    5
Vertebrate Orthology Source
Alliance of Genome Resources
  • Human Ortholog
    DHFR, dihydrofolate reductase
  • Synonyms
    DHFR1, DYR
  • Links
    NCBI Gene ID: 1719
    UniProt: P00374

  • Chr Location
    5q14.1; chr5:80626226-80654983 (-)  GRCh38
  • Human Ortholog
    DHFR2, dihydrofolate reductase 2
  • Synonyms
    DHFRL1, DHFRP4
  • Links
    NCBI Gene ID: 200895
    UniProt: Q86XF0

  • Chr Location
    3q11.2; chr3:94047836-94063389 (-)  GRCh38
Human Diseases
more
  • Diseases
    1 with human DHFR associations

Human Disease Mouse Models
      
IDs
Click on a disease name to see all genes associated with that disease.

Mutations,
Alleles, and
Phenotypes
less
  • Phenotype Summary
    23 phenotypes from 2 alleles in 2 genetic backgrounds
    22 phenotype references
Phenotype Overview

adipose tissue
behavior/neurological
cardiovascular system
cellular
craniofacial
digestive/alimentary system
embryo
endocrine/exocrine glands
growth/size/body
hearing/vestibular/ear
hematopoietic system
homeostasis/metabolism
integument
immune system
limbs/digits/tail
liver/biliary system
mortality/aging
muscle
nervous system
pigmentation
renal/urinary system
reproductive system
respiratory system
skeleton
taste/olfaction
neoplasm
vision/eye

Click cells to view annotations.
Mice homozygous for a knock-out allele exhibit lethality soon after E14.5 with reduced size, pallor, impaired hematopoiesis and abnormal folic acid levels. Mice heterozygous for this allele exhibit abnormal folic acid levels, abnormal hemopoiesis and reduced endothelium-dependent vasorelaxation.
Gene Ontology
(GO)
Classifications
less
  • All GO Annotations
    59
  • GO References
    21
Molecular Function

carbohydrate derivative binding
cytoskeletal protein binding
DNA binding
enzyme regulator
hydrolase
ligase
lipid binding
oxidoreductase
RNA binding
signaling receptor activity
signaling receptor binding
transcription
transferase
transporter
Biological Process

carbohydrate derivative metabolism
cell differentiation
cell population proliferation
cellular component organization
DNA-templated transcription
establishment of localization
homeostatic process
immune system process
lipid metabolic process
programmed cell death
protein metabolic process
response to stimulus
signaling
system development
Cellular Component

cell projection
cytoplasmic vesicle
cytoskeleton
cytosol
endoplasmic reticulum
endosome
extracellular region
Golgi apparatus
mitochondrion
membraneless organelle
nucleus
organelle envelope
organelle lumen
plasma membrane
protein-containing complex
synapse
vacuole
Click cells to view annotations.
Expression
less
Expression Overview

early conceptus
embryo ectoderm
embryo endoderm
embryo mesoderm
embryo mesenchyme
extraembryonic component
alimentary system
auditory system
branchial arches
cardiovascular system
connective tissue
endocrine system
exocrine system
hemolymphoid system
integumental system
limbs
liver and biliary system
musculoskeletal system
nervous system
olfactory system
reproductive system
respiratory system
urinary system
visual system
Click cells to view annotations.


  • Assay Results
    728
  • Tissues
    117
  • cDNA Data
    70
  • Literature Summary
    12
  • Comparison Matrix
    Gene Expression + Phenotype
    • Sequences &
    Gene Models
    less
    Representative SequencesLengthStrain/SpeciesFlank
    genomic 13361 NCBI Gene Model | MGI Sequence Detail 34271 C57BL/6J ±  kb
    transcript NM_010049 RefSeq | MGI Sequence Detail 5307 ZRU/MplStud  
    polypeptide P00375 UniProt | EBI | MGI Sequence Detail 187 Not Applicable  
    For the selected sequence
    Protein
    Information
    less
    Molecular
    Reagents
    less
    • All nucleic 80
      Genomic 3
      cDNA 71
      Primer pair 3
      Other 3
      Antibodies 1

      Microarray probesets 6
    Other
    Accession IDs
    less
    MGD-MRK-8841, MGI:1918808, MGI:2145281, MGI:2145455, MGI:3034358
    References
    more
    • Summaries
      All 132
      Developmental Gene Expression 12
      Gene Ontology 21
      Phenotypes 22
    • Earliest
      J:30524 HAKALA MT, et al., Relation of folic acid reductase to amethopterin resistance in cultured mammalian cells. J Biol Chem. 1961 Mar;236:952-8
    • Latest
      J:379041 Zhang Y, et al., Alleviation of accelerated diabetic atherogenesis in STZ-treated apoE/NOX1 DKO mice, apoE(-/-)/tg-EC-DHFR mice, and by folic acid. Redox Biol. 2025 Feb 27;82:103570
    Contributing Projects:
    Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
    Citing These Resources
    Funding Information
    Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
    Send questions and comments to User Support.     		last database update
    09/01/2026
    MGI 6.24     		The Jackson Laboratory