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Chrna4 Gene Detail
Summary
  • Symbol
    Chrna4
  • Name
    cholinergic receptor, nicotinic, alpha polypeptide 4
  • Synonyms
    a4 nicotinic receptor, Acra-4, Acra4, alpha4 nAChR, alpha4-nAChR
  • Feature Type
    protein coding gene
  • IDs
    MGI:87888
    NCBI Gene: 11438
  • Alliance
    genome feature page
  • Transcription Start Sites
    7 TSS
Location &
Maps
more
  • Sequence Map
    Chr2:180664104-180685339 bp, - strand
    From NCBI annotation of GRCm39
  • View this region in JBrowse
  • Genome Browsers
    Ensembl | UCSC | NCBI
  • Genetic Map
    Chromosome 2, 103.54 cM
  • Mapping Data
    12 experiments
Strain
Comparison
more
  • SNPs within 2kb
    568 from dbSNP Build 142
  • Strain Annotations
    19
  • RFLP
    3
For selected strains:
Strain Gene Model ID Feature Type Coordinates Select Strains
C57BL/6J MGI_C57BL6J_87888
 protein coding gene Chr2:180660173-180685339 (-)
129S1/SvImJ ENSMUSG00200001757
 protein coding gene Chr2:176682635-176707804 (-)
A/J ENSMUSG00195001080
 protein coding gene Chr2:176919830-176945015 (-)
AKR/J ENSMUSG00220000061
 protein coding gene Chr2:174598468-174623651 (-)
BALB/cJ ENSMUSG00180000464
 protein coding gene Chr2:174721847-174747038 (-)
C3H/HeJ ENSMUSG00175004216
 protein coding gene Chr2:175880420-175905600 (-)
C57BL/6NJ ENSMUSG00215002481
 protein coding gene Chr2:174822112-174847281 (-)
CAROLI/EiJ MGP_CAROLIEiJ_G0024795
 protein coding gene Chr2:170557569-170577191 (-)
CAST/EiJ ENSTCUG00005000749
 protein coding gene Chr2:174174952-174200152 (-)
CBA/J ENSMUSG00210001380
 protein coding gene Chr2:175200475-175225640 (-)
DBA/2J ENSMUSG00185019657
 protein coding gene Chr2:176439868-176465053 (-)
FVB/NJ ENSMUSG00205002203
 protein coding gene Chr2:175252202-175277376 (-)
JF1/MsJ ENSUMUG00000021622
 protein coding gene Chr2:177625121-177650255 (-)
LP/J ENSMUSG00230021296
 protein coding gene Chr2:178333578-178358746 (-)
NOD/ShiLtJ ENSMUSG00190015679
 protein coding gene Chr2:174742053-174767223 (-)
NZO/HlLtJ ENSMUSG00225006527
 protein coding gene Chr2:186420394-186445563 (-)
PWK/PhJ ENSLUMG00010000357
 protein coding gene Chr2:175051251-175076378 (-)
SPRET/EiJ ENSMSPG00010003907
 protein coding gene Chr2:177524722-177544927 (-)
WSB/EiJ ENSIUOG00005000165
 protein coding gene Chr2:174765841-174791038 (-)



Homology
more
  • Human Ortholog
    CHRNA4, cholinergic receptor nicotinic alpha 4 subunit
  • Vertebrate Orthologs
    3
Vertebrate Orthology Source
Alliance of Genome Resources
  • Human Ortholog
    CHRNA4, cholinergic receptor nicotinic alpha 4 subunit
  • Synonyms
    BFNC, EBN, EBN1, NACHR, NACHRA4, NACRA4
  • Links
    NCBI Gene ID: 1137
    UniProt: P43681

  • Chr Location
    20q13.33; chr20:63343223-63378401 (-)  GRCh38
Human Diseases
more
  • Diseases
    2 with human CHRNA4 associations

Human Disease Mouse Models
      
IDs
 View 1 "NOT" model
IDs
Click on a disease name to see all genes associated with that disease.

  • Mutations/Alleles
    1 with disease annotations
  • References
    3 with disease annotations
Mutations,
Alleles, and
Phenotypes
less
  • Phenotype Summary
    38 phenotypes from 10 alleles in 10 genetic backgrounds
    1 phenotype from multigenic genotypes
    71 phenotype references
Phenotype Overview

adipose tissue
behavior/neurological
cardiovascular system
cellular
craniofacial
digestive/alimentary system
embryo
endocrine/exocrine glands
growth/size/body
hearing/vestibular/ear
hematopoietic system
homeostasis/metabolism
integument
immune system
limbs/digits/tail
liver/biliary system
mortality/aging
muscle
nervous system
pigmentation
renal/urinary system
reproductive system
respiratory system
skeleton
taste/olfaction
neoplasm
vision/eye

Click cells to view annotations.
Nullizygous mice may show reduced chemically-elicited analgesia, susceptibility to seizures, increased anxiety, and altered behavioral responses to nicotine or a new environment. Homozygotes for any of several knock-in alleles exhibit altered nervous system physiology and/or sensitivity to nicotine.
Gene Ontology
(GO)
Classifications
less
  • All GO Annotations
    125
  • GO References
    29
Molecular Function

carbohydrate derivative binding
cytoskeletal protein binding
DNA binding
enzyme regulator
hydrolase
ligase
lipid binding
oxidoreductase
RNA binding
signaling receptor activity
signaling receptor binding
transcription
transferase
transporter
Biological Process

carbohydrate derivative metabolism
cell differentiation
cell population proliferation
cellular component organization
DNA-templated transcription
establishment of localization
homeostatic process
immune system process
lipid metabolic process
programmed cell death
protein metabolic process
response to stimulus
signaling
system development
Cellular Component

cell projection
cytoplasmic vesicle
cytoskeleton
cytosol
endoplasmic reticulum
endosome
extracellular region
Golgi apparatus
mitochondrion
membraneless organelle
nucleus
organelle envelope
organelle lumen
plasma membrane
protein-containing complex
synapse
vacuole
Click cells to view annotations.
Expression
less
Expression Overview

early conceptus
embryo ectoderm
embryo endoderm
embryo mesoderm
embryo mesenchyme
extraembryonic component
alimentary system
auditory system
branchial arches
cardiovascular system
connective tissue
endocrine system
exocrine system
hemolymphoid system
integumental system
limbs
liver and biliary system
musculoskeletal system
nervous system
olfactory system
reproductive system
respiratory system
urinary system
visual system
Click cells to view annotations.


  • Assay Results
    830
  • Tissues
    135
  • cDNA Data
    49
  • Literature Summary
    14
  • Comparison Matrix
    Gene Expression + Phenotype
    • Sequences &
    Gene Models
    less
    Representative SequencesLengthStrain/SpeciesFlank
    genomic 11438 NCBI Gene Model | MGI Sequence Detail 21236 C57BL/6J ±  kb
    transcript NM_015730 RefSeq | MGI Sequence Detail 4575 ZRU/MplStud  
    polypeptide O70174 UniProt | EBI | MGI Sequence Detail 629 Not Applicable  
    For the selected sequence
    Protein
    Information
    less
    • UniProt
      5 Sequences
    • Protein Ontology
      PR:000005457 neuronal acetylcholine receptor subunit alpha-4
      (term hierarchy)
    • InterPro Domains
      IPR038050 Neuronal acetylcholine receptor
      IPR006201 Neurotransmitter-gated ion-channel
      IPR018000 Neurotransmitter-gated ion-channel, conserved site
      IPR006202 Neurotransmitter-gated ion-channel ligand-binding domain
      IPR036734 Neurotransmitter-gated ion-channel ligand-binding domain superfamily
      IPR006029 Neurotransmitter-gated ion-channel transmembrane domain
      IPR036719 Neurotransmitter-gated ion-channel transmembrane domain superfamily
      IPR002394 Nicotinic acetylcholine receptor
    • GlyGen
      O70174 3 sites, 3 N-linked glycans (3 sites)
    Molecular
    Reagents
    less
    • All nucleic 57
      cDNA 52
      Primer pair 3
      Other 2

      Microarray probesets 6
    Other
    Accession IDs
    less
    MGD-MRK-1057, MGD-MRK-1061
    References
    more
    • Summaries
      All 159
      Developmental Gene Expression 14
      Diseases 3
      Gene Ontology 29
      Phenotypes 71
    • Earliest
      J:10489 Bessis A, et al., Chromosomal localization of the mouse genes coding for alpha 2, alpha 3, alpha 4 and beta 2 subunits of neuronal nicotinic acetylcholine receptor. FEBS Lett. 1990 May 7;264(1):48-52
    • Latest
      J:363864 Tetteh-Quarshie S, et al., The influence of high-fat diet on nicotine vapor self-administration, neuronal excitability, and leptin levels in adult mice. Physiol Behav. 2025 Apr 1;292:114823
    Contributing Projects:
    Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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    Send questions and comments to User Support.     		last database update
    09/08/2026
    MGI 6.24     		The Jackson Laboratory