About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:236149 Edwards W, et al., Identification of a novel progenitor cell marker, grainyhead-like 2 in the developing pituitary. Dev Dyn. 2016 Nov;245(11):1097-1106
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6155596
Gene symbol: Grhl2
Gene name: grainyhead like transcription factor 2
Antibody: anti-GRHL2
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to Cy3
Assay notes: A tyramide signal amplification was used. Formaldehyde fixative was 3.7% or a Zinc fixative was used.
Results
Specimen 1A: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: pituitary gland Present 1A Expression is in the vast majority of cells.
Specimen 1B: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: pituitary gland Present 1B Expression is in the vast majority of cells.
Specimen 1C: postnatal day 1 (more )
Structure Level Pattern Image Note
TS27: pituitary gland Present Regionally restricted 1C Expression is restricted to the marginal cells lining the lumen.
TS27: adenohypophysis Present Single cells 1C Expression is scattered.
TS27: adenohypophysis pars intermedia Present Single cells 1C Expression is scattered in the dorsal intermediate lobe.
Specimen 1D: postnatal day 5 (more )
Structure Level Pattern Image Note
TS28: adenohypophysis Weak Regionally restricted 1D Expression is in lining the cleft.
TS28: adenohypophysis pars intermedia Weak Regionally restricted 1D Expression is in lining the cleft.
Specimen 1E: postnatal day 30 (more )
Structure Level Pattern Image Note
TS28: adenohypophysis Weak 1E Expression is lining the cleft.
TS28: adenohypophysis pars intermedia Weak 1E Expression is lining the cleft.
Specimen 2A: postnatal day 1 (more )
Note: Double labeled: magenta - Grhl2; green - Sox2 (assay MGI:6155656).
Structure Level Pattern Image Note
TS27: adenohypophysis pars intermedia Present 2C , 2A
Specimen 2D: postnatal day 1 (more )
Note: Double labeled: magenta - Grhl2; green - Sox2 (assay MGI:6155656).
Structure Level Pattern Image Note
TS27: adenohypophysis Present Regionally restricted 2F , 2D Expression is in the cleft cells.
Specimen 2G: postnatal day 1 (more )
Note: Double labeled: magenta - Grhl2; green - Sox2 (assay MGI:6155656).
Structure Level Pattern Image Note
TS27: adenohypophysis Present Single cells 2I , 2G Expression is in the parenchyma.
TS27: adenohypophysis pars intermedia Present Regionally restricted 2G Expression is in a few cells of the dorsal intermediate lobe.
Specimen 2J: postnatal day 1 (more )
Note: Double labeled: magenta - Grhl2; green - Pomc (assay MGI:6155742).
Structure Level Pattern Image Note
TS27: adenohypophysis Present Single cells 2L , 2J Expression is in the parenchyma.
TS27: adenohypophysis pars intermedia Present Single cells 2J , 2L Expression is in a few cells of the dorsal intermediate lobe.
Specimen 2M: postnatal day 1 (more )
Note: Double labeled: magenta - Grhl2; green - Pomc (assay MGI:6155742).
Structure Level Pattern Image Note
TS27: adenohypophysis pars intermedia Present 2O'' , 2M , 2O , 2O'
Specimen 2P: postnatal day 1 (more )
Note: Double labeled: magenta - Grhl2; green - Pou1f1 (assay MGI:6155745).
Structure Level Pattern Image Note
TS27: adenohypophysis Present Single cells 2P , 2R Expression is in the parenchyma.
TS27: adenohypophysis pars intermedia Present Regionally restricted 2R , 2P Expression is in a few cells of the dorsal intermediate lobe.
Specimen 2S: postnatal day 1 (more )
Note: Double labeled: magenta - Grhl2; green - Lhb (assay MGI:6155751).
Structure Level Pattern Image Note
TS27: adenohypophysis Present Regionally restricted 2S , 2U Expression is in the cleft cells.
Specimen 3A: postnatal day 5 (more )
Structure Level Pattern Image Note
TS28: adenohypophysis Present Regionally restricted 3A Expression is mainly in the cleft cells.
TS28: adenohypophysis pars intermedia Present Regionally restricted 3A Expression is mainly in the cleft cells.
Specimen 3B: postnatal day 5; Prop1df/Prop1df (more )
Structure Level Pattern Image Note
TS28: adenohypophysis Strong Regionally restricted 3B Expression at the cleft has increased in this mutant.
Specimen 3C: postnatal day 5 (more )
Structure Level Pattern Image Note
TS28: adenohypophysis Present Regionally restricted 3C Expression is mainly in the cleft cells.
TS28: adenohypophysis pars intermedia Present Regionally restricted 3C Expression is mainly in the cleft cells.
Specimen 3D: postnatal day 5; Prop1df/Prop1df (more )
Structure Level Pattern Image Note
TS28: adenohypophysis Strong Regionally restricted 3D Expression at the cleft has increased in this mutant.
Specimen 4A: postnatal day 1 (more )
Structure Level Pattern Image Note
TS27: adenohypophysis pars intermedia Present Regionally restricted 4B , 4A Expression is in the cleft cells.
TS27: adenohypophysis Present Regionally restricted 4A , 4C Expression is in the cleft cells and scattered in the parenchyma.
Specimen 4D: postnatal day 1; Foxg1tm1(cre)Skm/Foxg1+, Notch2tm3Grid/Notch2tm3Grid (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS27: adenohypophysis pars intermedia Weak Regionally restricted 4D , 4E Expression is in the cleft cells.
TS27: adenohypophysis Present Regionally restricted 4D , 4F Expression in the cleft cells is the same as in wild-type.
Specimen 6B: postnatal day 1 (more )
Note: Double labeled: red - Grhl2; green - Cdh1 (assay MGI:6156393).
Structure Level Pattern Image Note
TS27: adenohypophysis Present Regionally restricted 6B Expression is in the cleft cells.
TS27: adenohypophysis pars intermedia Present Regionally restricted 6B Expression is in the cleft cells.
Specimen 6D: postnatal day 1 (more )
Note: Double labeled: red - Grhl2; green - Cdh1 (assay MGI:6156393).
Structure Level Pattern Image Note
TS27: adenohypophysis pars intermedia Present Regionally restricted 6D Expression is in the cleft cells.
Specimen 6F: postnatal day 1 (more )
Note: Double labeled: red - Grhl2; green - Cdh1 (assay MGI:6156393).
Structure Level Pattern Image Note
TS27: adenohypophysis Present Regionally restricted 6F Expression is in the cleft cells.
Specimen 6H: postnatal day 1; Foxg1tm1(cre)Skm/Foxg1+, Notch2tm3Grid/Notch2tm3Grid (more )
Note: Conditional mutant. Double labeled: red - Grhl2; green - Cdh1 (assay MGI:6156393).
Structure Level Pattern Image Note
TS27: adenohypophysis Present Regionally restricted 6H Expression in the cleft cells is the same as in wild-type.
TS27: adenohypophysis pars intermedia Weak Regionally restricted 6H Expression is in the cleft cells.
Specimen 6J: postnatal day 1; Foxg1tm1(cre)Skm/Foxg1+, Notch2tm3Grid/Notch2tm3Grid (more )
Note: Conditional mutant. Double labeled: red - Grhl2; green - Cdh1 (assay MGI:6156393).
Structure Level Pattern Image Note
TS27: adenohypophysis pars intermedia Weak Regionally restricted 6J Expression is in the cleft cells.
Specimen 6L: postnatal day 1; Foxg1tm1(cre)Skm/Foxg1+, Notch2tm3Grid/Notch2tm3Grid (more )
Note: Conditional mutant. Double labeled: red - Grhl2; green - Cdh1 (assay MGI:6156393).
Structure Level Pattern Image Note
TS27: adenohypophysis Present Regionally restricted 6L Expression in the cleft cells is the same as in wild-type.
Specimen 7B: postnatal day 1 (more )
Note: Double labeled: red - Grhl2; green - Pcna (assay MGI:6156396).
Structure Level Pattern Image Note
TS27: adenohypophysis pars intermedia Present Regionally restricted 7B Expression is in the cleft cells.
TS27: adenohypophysis Present Regionally restricted 7B Expression is in the cleft cells and scattered in the parenchyma.
Specimen 7D: postnatal day 1 (more )
Note: Double labeled: red - Grhl2; green - Pcna (assay MGI:6156396).
Structure Level Pattern Image Note
TS27: adenohypophysis pars intermedia Present Regionally restricted 7D Expression is in the cleft cells.
Specimen 7F: postnatal day 1 (more )
Note: Double labeled: red - Grhl2; green - Pcna (assay MGI:6156396).
Structure Level Pattern Image Note
TS27: adenohypophysis Present Regionally restricted 7F Expression is in the cleft cells and scattered in the parenchyma.
Specimen 7H: postnatal day 1; Foxg1tm1(cre)Skm/Foxg1+, Notch2tm3Grid/Notch2tm3Grid (more )
Note: Conditional mutant. Double labeled: red - Grhl2; green - Pcna (assay MGI:6156396).
Structure Level Pattern Image Note
TS27: adenohypophysis pars intermedia Weak Regionally restricted 7H Expression is in the cleft cells.
TS27: adenohypophysis Present Regionally restricted 7H Expression in the cleft cells is the same as in wild-type.
Specimen 7J: postnatal day 1; Foxg1tm1(cre)Skm/Foxg1+, Notch2tm3Grid/Notch2tm3Grid (more )
Note: Conditional mutant. Double labeled: red - Grhl2; green - Pcna (assay MGI:6156396).
Structure Level Pattern Image Note
TS27: adenohypophysis pars intermedia Weak Regionally restricted 7J Expression is in the cleft cells.
Specimen 7L: postnatal day 1; Foxg1tm1(cre)Skm/Foxg1+, Notch2tm3Grid/Notch2tm3Grid (more )
Note: Conditional mutant. Double labeled: red - Grhl2; green - Pcna (assay MGI:6156396).
Structure Level Pattern Image Note
TS27: adenohypophysis Present Regionally restricted 7L Expression in the cleft cells is the same as in wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
04/16/2024
MGI 6.23 		The Jackson Laboratory