Analysis Tools
immune system
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• Lgals-deficient mice display attenuated peritoneal inflammation compared to wild-type upon stimulation with thioglycollate broth administration
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• mutants show a higher percentage of eosinophils on days 1, 2 and 4 after thioglycollate broth treatment
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• null mice have lower numbers of lymphocytes on days 2 and 4 after treatment
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• the % of testicular macrophages within the population of CD45+ leukocytes is reduced by 11% in adult males
(J:313172)
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• on day 1 after treatment mutants have a lower percentage of monocytes/macrophages
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• leukocytes from mutants show weaker NF-kappa b binding activity upon stimulation with thioglycollate broth than wild-type controls
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• peritoneal macrophages show decreased areas of adherence in culture compared to wild-type
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• Lgals-deficient macrophages are more sensitive to apoptotic stimuli in culture
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hematopoietic system
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• mutants show a higher percentage of eosinophils on days 1, 2 and 4 after thioglycollate broth treatment
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• null mice have lower numbers of lymphocytes on days 2 and 4 after treatment
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• the % of testicular macrophages within the population of CD45+ leukocytes is reduced by 11% in adult males
(J:313172)
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• on day 1 after treatment mutants have a lower percentage of monocytes/macrophages
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• leukocytes from mutants show weaker NF-kappa b binding activity upon stimulation with thioglycollate broth than wild-type controls
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• peritoneal macrophages show decreased areas of adherence in culture compared to wild-type
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• Lgals-deficient macrophages are more sensitive to apoptotic stimuli in culture
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digestive/alimentary system
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• Lgals-deficient mice display attenuated peritoneal inflammation compared to wild-type upon stimulation with thioglycollate broth administration
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cellular
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• testes show a significant reduction in male germ cell number at P5 and P15
• reduced number of gonocytes and spermatogonia is independent of proliferation
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• Lgals-deficient macrophages are more sensitive to apoptotic stimuli in culture
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• at P5, P35, P50 and P100, testes exhibit a significantly higher number of TUNEL+ apoptotic germ cells than wild-type testes
• at P50, testes show downregulated expression of the anti-apoptotic protein BCL2 but upregulated expression of the proapoptotic proteins BAK1 and BAX, indicating an imbalance in anti-/pro-apoptotic protein expression
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reproductive system
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• testes show a significant reduction in male germ cell number at P5 and P15
• reduced number of gonocytes and spermatogonia is independent of proliferation
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• at P5, P35, P50 and P100, testes exhibit a significantly higher number of TUNEL+ apoptotic germ cells than wild-type testes
• at P50, testes show downregulated expression of the anti-apoptotic protein BCL2 but upregulated expression of the proapoptotic proteins BAK1 and BAX, indicating an imbalance in anti-/pro-apoptotic protein expression
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• at P5, the number of seminiferous tubules without germ cells is significantly increased compared to wild-type testes (46% versus 13%, respectively)
• at P15, the % of tubules containing a lumen is 2-fold lower than in wild-type tubules
• 8% of tubules at P15 and ~15% of tubules at P20 contain no germ cells
• however, by P35, all seminiferous tubules contain germ cells
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• Sertoli cell maturation is delayed, as shown by 3-fold increase in the mRNA levels of anti-Mullerian hormone (Amh) at P15
• however, at P15, the overall number of Sertoli cells is unaffected
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• at P50 and P100, testicular weight is significantly lower than in wild-type males
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• adult males exhibit mild testicular atrophy
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• gene and protein expression of Insl3 (insulin-like-3, a Leydig cell marker) and enzymes involved in steroid biosynthesis are significantly upregulated in adult testes
• the % of testicular macrophages within the population of CD45+ leukocytes is reduced by 11% in adult males
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• males show a delay in the first wave of spermatogenesis
• at P15, most of the differentiated germ cells consist of zygotene spermatocytes with numerous tubules containing only spermatogonia; the % of tubules containing pachytene spermatocytes is significantly lower than in wild-type testes (9% versus 23%, respectively)
• at P20, most tubules contain only pachytene spermatocytes (a mid-meiotic stage); the % of tubules containing round spermatids and pachytene spermatocytes is significantly lower than in wild-type testes (3.6% versus 17% and 53% versus 82%, respectively)
• by P20, the % of tubules containing less-differentiated cells such as leptotene/zygotene spermatocytes or spermatogonia) is significantly higher than in wild-type testes (14% versus 3.7% and 18% versus 0%, respectively)
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homeostasis/metabolism
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• at P50, serum testosterone levels are significantly higher than in wild-type males
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endocrine/exocrine glands
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• at P5, the number of seminiferous tubules without germ cells is significantly increased compared to wild-type testes (46% versus 13%, respectively)
• at P15, the % of tubules containing a lumen is 2-fold lower than in wild-type tubules
• 8% of tubules at P15 and ~15% of tubules at P20 contain no germ cells
• however, by P35, all seminiferous tubules contain germ cells
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• Sertoli cell maturation is delayed, as shown by 3-fold increase in the mRNA levels of anti-Mullerian hormone (Amh) at P15
• however, at P15, the overall number of Sertoli cells is unaffected
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• at P50 and P100, testicular weight is significantly lower than in wild-type males
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• adult males exhibit mild testicular atrophy
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• gene and protein expression of Insl3 (insulin-like-3, a Leydig cell marker) and enzymes involved in steroid biosynthesis are significantly upregulated in adult testes
• the % of testicular macrophages within the population of CD45+ leukocytes is reduced by 11% in adult males
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