Phenotypes associated with this allele

• Allele Symbol: Wls^tm1.1Lan
• Allele Name: targeted mutation 1.1, Richard A Lang
• Allele ID: MGI:4452362
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Tg(KRT5-rtTA)#Glk/0 Tg(tetO-cre)1Jaw/0 Wls^tm1.1Lan/Wls^tm1.1Lan	involves: 129S6/SvEvTac * C57BL/6 * FVB/N	MGI:5538309
cn2	Tg(Krt1-15-cre/PGR*)22Cot/0 Wls^tm1.1Lan/Wls^tm1.1Lan	involves: 129S6/SvEvTac * C57BL/6 * SJL	MGI:5538317
cn3	Wls^tm1.1Lan/Wls^tm1.1Lan H2az2^Tg(Wnt1-cre)11Rth/H2az2^+	involves: 129S/SvEv * C57BL/6J * CBA/J * SJL	MGI:4838405
cn4	Wls^tm1.1Lan/Wls^tm1.1Lan Tg(EIIa-cre)C5379Lmgd/0	involves: 129S/SvEv * FVB/N * SJL	MGI:4838404
cn5	Wls^tm1.1Lan/Wls^tm1.1Lan Tg(Pdx1-cre)6Tuv/0	involves: 129S/SvEv * FVB/N * SJL	MGI:4838406

Genotype
MGI:5538309

cn1

• Allelic Composition: Tg(KRT5-rtTA)#Glk/0; Tg(tetO-cre)1Jaw/0; Wls^tm1.1Lan/Wls^tm1.1Lan
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

integument

abnormal hair follicle morphology ( J:204142 )

• in P60 animals, hair follicle structures are retained in absence of external hair, but display abnormalities including cyst formation

abnormal hair cycle catagen phase ( J:204142 )

• cre induction at P4 causes hair follicle regression by P14 without loss of bulge stem cell markers

abnormal epidermal layer morphology ( J:204142 )

• when doxycline induction is performed from P4 to P18, epidermis exhibits increased proliferation as well as expanded expression of basal and suprabasal markers and hyperproliferation markers

• when doxycline induction is performed from P4 to P18, enlargement of intercellular spaces and some cell membrane protrusions are observed in some areas of mutant skins at P60

• defects of the interfollicular epidermis similar to those observed in conditional Ctnnb1 mutants are seen in induced mutants

abnormal keratinocyte morphology ( J:204142 )

immune system

cutaneous mastocytosis ( J:204142 )

• with doxycycline induction from P4-P18, mast cell number in the dermis is increased at P60

hematopoietic system

cutaneous mastocytosis ( J:204142 )

• with doxycycline induction from P4-P18, mast cell number in the dermis is increased at P60

Genotype
MGI:5538317

cn2

• Allelic Composition: Tg(Krt1-15-cre/PGR*)22Cot/0; Wls^tm1.1Lan/Wls^tm1.1Lan
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * SJL

integument

integument phenotype ( J:204142 )

N • hyperproliferation is not observed in the epidermis with RU486 treatment from P20-P27

Genotype
MGI:4838405

cn3

• Allelic Composition: Wls^tm1.1Lan/Wls^tm1.1Lan; H2az2^{Tg(Wnt1-cre)11Rth}/H2az2⁺
• Genetic Background: involves: 129S/SvEv * C57BL/6J * CBA/J * SJL

mortality/aging

perinatal lethality ( J:164701 )

embryo

short rostral-caudal axis ( J:164701 )

• mutant mice exhibit a shortened anteroposterior axis

abnormal neural tube morphology ( J:164701 )

• mutant phenotype is obvious as early as E9.5 due to the presence of a shortened neural tube

nervous system

abnormal neural tube morphology ( J:164701 )

• mutant phenotype is obvious as early as E9.5 due to the presence of a shortened neural tube

absent midbrain-hindbrain boundary ( J:164701 )

• mutant mice exhibit no apparent isthmus

decreased forebrain size ( J:164701 )

• at E12.5 a severely truncated forebrain

absent choroid plexus ( J:164701 )

• at E12.5

absent midbrain ( J:164701 )

• absent midbrain

abnormal metencephalon morphology ( J:164701 )

• at E10.5 verified deletion of regions of the metencephalon

absent cerebellum ( J:164701 )

• at E12.5

Genotype
MGI:4838404

cn4

• Allelic Composition: Wls^tm1.1Lan/Wls^tm1.1Lan; Tg(EIIa-cre)C5379Lmgd/0
• Genetic Background: involves: 129S/SvEv * FVB/N * SJL

mortality/aging

embryonic lethality between implantation and somite formation, incomplete penetrance ( J:164701 )

• at E8.5, 9 of 10 embryos are in the process of being reabsorbed

embryo

embryonic growth arrest ( J:164701 )

• germline homozygotes appear arrested at E6, they do not progress past the egg cylinder stage, and by morphological criteria lack mesoderm

failure of primitive streak formation ( J:164701 )

Genotype
MGI:4838406

cn5

• Allelic Composition: Wls^tm1.1Lan/Wls^tm1.1Lan; Tg(Pdx1-cre)6Tuv/0
• Genetic Background: involves: 129S/SvEv * FVB/N * SJL

endocrine/exocrine glands

pancreas hypoplasia ( J:164701 )