Tg(Krt1-15-cre/PGR*)22Cot
Transgene Detail

Summary

• Symbol: Tg(Krt1-15-cre/PGR*)22Cot
• Name: transgene insertion 22, George Cotsarelis
• MGI ID: MGI:3511785
• Synonyms: K15CrePR, K15-CrePR1, Krt15-cre/PGR)22Cot, Tg(Krt1-15-cre/PGR)22Cot
• Transgene: Tg(Krt1-15-cre/PGR*)22Cot Location: unknown
• Alliance: Tg(Krt1-15-cre/PGR*)22Cot page

Transgene origin

• Strain of Origin: (C57BL/6J x SJL/J)F2

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: mifepristone
• Mutation: Insertion
• Tg(Krt1-15-cre/PGR*)22Cot expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Krt15 (MGI:96689)

• Mutation details: The transgene comprises the entire 5.0-kb mouse Krt1-15 promoter region driving expression of a fusion protein composed of an amino-terminal nuclear localization signal followed by Cre recombinase joined to the carboxyl-terminal hormone-binding domain (HBD) of a mutant human progesterone receptor. This mutant HBD, whose carboxyl-terminal is truncated by 42 amino acids, fails to bind endogenous progestins but binds and, paradoxically, is activated by progesterone receptor antagonists such as RU486. Constitutive expression of the fusion protein is directed by the Krt1-15 promoter to epithelial stem cells in the bulge region of the hair follicle. It remains inactive in the cytoplasm until bound to RU486, whereupon it translocates to the nucleus and can catalyze loxP-mediated recombination. (J:84610, J:94076, J:94082)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Krt15 (mouse) Summary of all recombinase alleles driven by Krt15. Comparative matrix view of recombinase activities Alliance mouse Krt15 gene page

Disease models

Tumor Data

List all tumor models in MMHCdb carrying Tg(Krt1-15-cre/PGR*)22Cot

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

Notes

This transgenic line is one of seven founder lines expressing the transgene, as assessed by RT-PCR. Mice of each line were crossed to mice expressing a beta-galactosidase Cre reporter transgene. Following topical application of RU486, bitransgenic mice of lines 22, 42 and 49 were shown to exhibit beta-galactosidase activity in their skin. Line 22, which showed the least enzyme activity in untreated skin, was selected for further analysis.

References

• Original: J:94076 Morris RJ, et al., Capturing and profiling adult hair follicle stem cells. Nat Biotechnol. 2004 Apr;22(4):411-7
• All: 76 reference(s)