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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Lgr5tm1(cre/ERT2)Cle
targeted mutation 1, Hans Clevers
MGI:3764660
Summary 21 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Rnf43tm1Cle/Rnf43tm1Cle
Znrf3tm1Cle/Znrf3tm1Cle
Lgr5tm1(cre/ERT2)Cle/Lgr5+
involves: 129P2/OlaHsd MGI:5439368
cn2
Slc39a7tm1.1Tfk/Slc39a7tm1.1Tfk
Lgr5tm1(cre/ERT2)Cle/Lgr5+
involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 MGI:5902448
cn3
TigarGt(EUCE0047g05)Hmgu/TigarGt(EUCE0047g05)Hmgu
Apctm1Tno/Apctm1Tno
Lgr5tm1(cre/ERT2)Cle/Lgr5+
involves: 129P2/OlaHsd * 129S4/SvJae MGI:5522716
cn4
Apctm1Tno/Apctm1Tno
Lgr5tm1(cre/ERT2)Cle/Lgr5+
involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6 MGI:5898006
cn5
Apctm1Tno/Apctm1Tno
Elp3tm1.1Tac/Elp3tm1.1Tac
Lgr5tm1(cre/ERT2)Cle/Lgr5+
involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6 * C57BL/6NTac MGI:5898005
cn6
Col1a1tm2(tetO-Pou5f1)Jae/Col1a1+
Gt(ROSA)26Sortm1(tTA)Roos/Gt(ROSA)26Sor+
Lgr5tm1(cre/ERT2)Cle/Lgr5+
involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6 * DBA/2J MGI:5508640
cn7
Col1a1tm1(tetO-SOX2)Mjm/Col1a1+
Gt(ROSA)26Sortm1(tTA)Roos/Gt(ROSA)26Sor+
Lgr5tm1(cre/ERT2)Cle/Lgr5+
involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6 * DBA/2J MGI:5508638
cn8
Gata6osem3Zfa/Gata6osem3Zfa
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Lgr5tm1(cre/ERT2)Cle/Lgr5+
involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6 MGI:6367573
cn9
Gata6osem1Zfa/Gata6osem1Zfa
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Lgr5tm1(cre/ERT2)Cle/Lgr5+
involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6 MGI:6367570
cn10
Nrbp1tm1.1Dja/Nrbp1tm1.2Dja
Lgr5tm1(cre/ERT2)Cle/Lgr5+
involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6J MGI:5427571
cn11
Rab11atm1.1Ngao/Rab11atm1.1Ngao
Lgr5tm1(cre/ERT2)Cle/Lgr5+
involves: 129P2/OlaHsd * 129S6/SvEvTac MGI:5608789
cn12
Ctnnb1tm1Mmt/Ctnnb1+
Lgr5tm1(cre/ERT2)Cle/Lgr5+
involves: 129P2/OlaHsd * 129X1/SvJ MGI:5475209
cn13
Cdc42tm1Brak/Cdc42tm1Brak
Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+
Lgr5tm1(cre/ERT2)Cle/Lgr5+
involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6 MGI:5427870
cn14
Cdx2tm2Fbe/Cdx2tm2Fbe
Lgr5tm1(cre/ERT2)Cle/Lgr5+
involves: 129P2/OlaHsd * C57BL/6 MGI:5308972
cn15
Gata6osem1Zfa/Gata6osem1Zfa
Lgr5tm1(cre/ERT2)Cle/Lgr5+
involves: 129P2/OlaHsd * C57BL/6 MGI:6367565
cn16
Bptfem1Zfa/Bptfem1Zfa
Ehfem1Zfa/Ehfem1Zfa
Lgr5tm1(cre/ERT2)Cle/Lgr5+
involves: 129P2/OlaHsd * C57BL/6 MGI:6367579
cn17
Cdc42tm1Brak/Cdc42tm1Brak
Lgr5tm1(cre/ERT2)Cle/Lgr5+
involves: 129P2/OlaHsd * C57BL/6 MGI:5427869
cn18
Apctm2.1Cip/Apc+
Lgr5tm1(cre/ERT2)Cle/Lgr5+
involves: 129P2/OlaHsd * C57BL/6 MGI:5432137
cn19
Bptfem1Zfa/Bptfem1Zfa
Lgr5tm1(cre/ERT2)Cle/Lgr5+
involves: 129P2/OlaHsd * C57BL/6 MGI:6367576
cn20
Elp3tm1.1Tac/Elp3tm1.1Tac
Lgr5tm1(cre/ERT2)Cle/Lgr5+
involves: 129P2/OlaHsd * C57BL/6 * C57BL/6NTac MGI:5898004
cx21
Lgr5tm1(cre/ERT2)Cle/Lgr5+
Lrig1tm1.1(cre/ERT2)Rjc/Lrig1tm1.1(cre/ERT2)Rjc
involves: 129 * 129P2/OlaHsd * C57BL/6 MGI:5432135


Genotype
MGI:5439368
cn1
Allelic
Composition
Rnf43tm1Cle/Rnf43tm1Cle
Znrf3tm1Cle/Znrf3tm1Cle
Lgr5tm1(cre/ERT2)Cle/Lgr5+
Genetic
Background
involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lgr5tm1(cre/ERT2)Cle mutation (1 available); any Lgr5 mutation (23 available)
Rnf43tm1Cle mutation (0 available); any Rnf43 mutation (21 available)
Znrf3tm1Cle mutation (0 available); any Znrf3 mutation (27 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
digestive/alimentary system
• form after tamoxifen treatment and contain Olfm4+ stem cells and Paneth cells

neoplasm
• form after tamoxifen treatment and contain Olfm4+ stem cells and Paneth cells




Genotype
MGI:5902448
cn2
Allelic
Composition
Slc39a7tm1.1Tfk/Slc39a7tm1.1Tfk
Lgr5tm1(cre/ERT2)Cle/Lgr5+
Genetic
Background
involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lgr5tm1(cre/ERT2)Cle mutation (1 available); any Lgr5 mutation (23 available)
Slc39a7tm1.1Tfk mutation (0 available); any Slc39a7 mutation (10 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• all tamoxifen-treated mice die within 4 days of gamma irradiation

digestive/alimentary system
N
• tamoxifen-treated mice exhibit normal epithelial organoid and crypt-derived epithelial organoid formation
• tamoxifen-treated mice exhibit massive apoptosis of transit-amplifying cells due to increased ER stress
• degeneration of Paneth cells in tamoxifen-treated mice

homeostasis/metabolism
• all tamoxifen-treated mice die within 4 days of gamma irradiation

endocrine/exocrine glands
• degeneration of Paneth cells in tamoxifen-treated mice




Genotype
MGI:5522716
cn3
Allelic
Composition
TigarGt(EUCE0047g05)Hmgu/TigarGt(EUCE0047g05)Hmgu
Apctm1Tno/Apctm1Tno
Lgr5tm1(cre/ERT2)Cle/Lgr5+
Genetic
Background
involves: 129P2/OlaHsd * 129S4/SvJae
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apctm1Tno mutation (5 available); any Apc mutation (57 available)
Lgr5tm1(cre/ERT2)Cle mutation (1 available); any Lgr5 mutation (23 available)
TigarGt(EUCE0047g05)Hmgu mutation (0 available); any Tigar mutation (42 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• in tamoxifen-treated mice compared with Apctm1Tno/Apctm1Tno Lgr5tm1(cre/ERT2)Cle/Lgr5+ mice

neoplasm
• tamoxifen-treated mice exhibit a reduced total tumor burden and average tumor size of abnormally proliferating adenomas in the small intestine and reduced size, but not number, of colon adenomas due to reduced proliferation and increased reactive oxygen species damage compared with Apctm1Tno/Apctm1Tno Lgr5tm1(cre/ERT2)Cle/Lgr5+ mice




Genotype
MGI:5898006
cn4
Allelic
Composition
Apctm1Tno/Apctm1Tno
Lgr5tm1(cre/ERT2)Cle/Lgr5+
Genetic
Background
involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apctm1Tno mutation (5 available); any Apc mutation (57 available)
Lgr5tm1(cre/ERT2)Cle mutation (1 available); any Lgr5 mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
digestive/alimentary system
• tamoxifen treated mice develop numerous hyperplasias in the intestine

neoplasm
• tamoxifen treated mice develop numerous hyperplasias in the intestine




Genotype
MGI:5898005
cn5
Allelic
Composition
Apctm1Tno/Apctm1Tno
Elp3tm1.1Tac/Elp3tm1.1Tac
Lgr5tm1(cre/ERT2)Cle/Lgr5+
Genetic
Background
involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6 * C57BL/6NTac
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apctm1Tno mutation (5 available); any Apc mutation (57 available)
Elp3tm1.1Tac mutation (0 available); any Elp3 mutation (27 available)
Lgr5tm1(cre/ERT2)Cle mutation (1 available); any Lgr5 mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
digestive/alimentary system
• the amount of Dclk1+/Sox9+ cells in the Lgr5+ population is decreased in ex vivo organoids
• intestinal crypts fail to efficiently generate spheroid structures ex vivo

neoplasm
• tamoxifen treated mice exhibit decreased numbers of hyperplastic foci/polyps in the intestine indicating impaired tumor initiation




Genotype
MGI:5508640
cn6
Allelic
Composition
Col1a1tm2(tetO-Pou5f1)Jae/Col1a1+
Gt(ROSA)26Sortm1(tTA)Roos/Gt(ROSA)26Sor+
Lgr5tm1(cre/ERT2)Cle/Lgr5+
Genetic
Background
involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6 * DBA/2J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Col1a1tm2(tetO-Pou5f1)Jae mutation (1 available); any Col1a1 mutation (126 available)
Gt(ROSA)26Sortm1(tTA)Roos mutation (3 available); any Gt(ROSA)26Sor mutation (510 available)
Lgr5tm1(cre/ERT2)Cle mutation (1 available); any Lgr5 mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
digestive/alimentary system
• expanded crypts in the ileum of doxycycline-treated mice
• increased cell proliferation in the intestine of doxycycline-treated mice without an increase in Lgr5+ cells

cellular
• in the intestine of doxycycline-treated mice without an increase in Lgr5+ cells

endocrine/exocrine glands
• expanded crypts in the ileum of doxycycline-treated mice




Genotype
MGI:5508638
cn7
Allelic
Composition
Col1a1tm1(tetO-SOX2)Mjm/Col1a1+
Gt(ROSA)26Sortm1(tTA)Roos/Gt(ROSA)26Sor+
Lgr5tm1(cre/ERT2)Cle/Lgr5+
Genetic
Background
involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6 * DBA/2J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Col1a1tm1(tetO-SOX2)Mjm mutation (0 available); any Col1a1 mutation (126 available)
Gt(ROSA)26Sortm1(tTA)Roos mutation (3 available); any Gt(ROSA)26Sor mutation (510 available)
Lgr5tm1(cre/ERT2)Cle mutation (1 available); any Lgr5 mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
digestive/alimentary system
• lyzsozyme+ Paneth cells in doxycycline-treated mice are not restricted to the base of the crypt unlike in control cells
• expanded crypts in the ileum of doxycycline-treated mice
• increased cell proliferation in the intestine of doxycycline-treated mice with an increase in intestinal stem Lgr5+ cells
• increased proliferation of intestinal stem cells in the intestine of doxycycline-treated mice
• expansion of intestinal stem cells is cell autonomous in doxycycline treated mice

cellular
• in the intestine of doxycycline-treated mice with an increase in Lgr5+ intestinal stem cells
• increased proliferation of intestinal stem cells in the intestine of doxycycline-treated mice
• expansion of intestinal stem cells is cell autonomous in doxycycline treated mice

endocrine/exocrine glands
• lyzsozyme+ Paneth cells in doxycycline-treated mice are not restricted to the base of the crypt unlike in control cells
• expanded crypts in the ileum of doxycycline-treated mice




Genotype
MGI:6367573
cn8
Allelic
Composition
Gata6osem3Zfa/Gata6osem3Zfa
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Lgr5tm1(cre/ERT2)Cle/Lgr5+
Genetic
Background
involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gata6osem3Zfa mutation (0 available); any Gata6os mutation (0 available)
Gt(ROSA)26Sortm1Sor mutation (7 available); any Gt(ROSA)26Sor mutation (510 available)
Lgr5tm1(cre/ERT2)Cle mutation (1 available); any Lgr5 mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• reduced cycling and proliferation of intestinal stem cells
• however, proliferation rates are normal

digestive/alimentary system
• reduced cycling and proliferation of intestinal stem cells
• however, proliferation rates are normal




Genotype
MGI:6367570
cn9
Allelic
Composition
Gata6osem1Zfa/Gata6osem1Zfa
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Lgr5tm1(cre/ERT2)Cle/Lgr5+
Genetic
Background
involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gata6osem1Zfa mutation (0 available); any Gata6os mutation (0 available)
Gt(ROSA)26Sortm1Sor mutation (7 available); any Gt(ROSA)26Sor mutation (510 available)
Lgr5tm1(cre/ERT2)Cle mutation (1 available); any Lgr5 mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
digestive/alimentary system
• impaired intestinal epithelial renewal in tamoxifen treated mice




Genotype
MGI:5427571
cn10
Allelic
Composition
Nrbp1tm1.1Dja/Nrbp1tm1.2Dja
Lgr5tm1(cre/ERT2)Cle/Lgr5+
Genetic
Background
involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lgr5tm1(cre/ERT2)Cle mutation (1 available); any Lgr5 mutation (23 available)
Nrbp1tm1.1Dja mutation (0 available); any Nrbp1 mutation (8 available)
Nrbp1tm1.2Dja mutation (0 available); any Nrbp1 mutation (8 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• abnormal goblet cell production in tamoxifen-treated mice

digestive/alimentary system
• authors state that tamoxifen-treated mice exhibit similar phenotype as Nrbp1tm1.1Dja/Nrbp1tm1.1Dja Gt(ROSA)26Sortm1(cre/ERT)Brn/ Gt(ROSA)26Sor+ mice
• abnormal goblet cell production in tamoxifen-treated mice
• abnormal granularization of Paneth cells in tamoxifen-treated mice
• abnormal localization of Paneth cells in tamoxifen-treated mice

endocrine/exocrine glands
• abnormal granularization of Paneth cells in tamoxifen-treated mice
• abnormal localization of Paneth cells in tamoxifen-treated mice




Genotype
MGI:5608789
cn11
Allelic
Composition
Rab11atm1.1Ngao/Rab11atm1.1Ngao
Lgr5tm1(cre/ERT2)Cle/Lgr5+
Genetic
Background
involves: 129P2/OlaHsd * 129S6/SvEvTac
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lgr5tm1(cre/ERT2)Cle mutation (1 available); any Lgr5 mutation (23 available)
Rab11atm1.1Ngao mutation (1 available); any Rab11a mutation (9 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
digestive/alimentary system
• increase in proliferation of intestinal epithelial stem cells and the transit-amplifying cells following tamoxifen induction




Genotype
MGI:5475209
cn12
Allelic
Composition
Ctnnb1tm1Mmt/Ctnnb1+
Lgr5tm1(cre/ERT2)Cle/Lgr5+
Genetic
Background
involves: 129P2/OlaHsd * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ctnnb1tm1Mmt mutation (0 available); any Ctnnb1 mutation (22 available)
Lgr5tm1(cre/ERT2)Cle mutation (1 available); any Lgr5 mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
digestive/alimentary system
• 40 days after tamoxifen treatment, mice have developed numerous polyps




Genotype
MGI:5427870
cn13
Allelic
Composition
Cdc42tm1Brak/Cdc42tm1Brak
Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+
Lgr5tm1(cre/ERT2)Cle/Lgr5+
Genetic
Background
involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdc42tm1Brak mutation (0 available); any Cdc42 mutation (35 available)
Gt(ROSA)26Sortm1(EYFP)Cos mutation (6 available); any Gt(ROSA)26Sor mutation (510 available)
Lgr5tm1(cre/ERT2)Cle mutation (1 available); any Lgr5 mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
digestive/alimentary system
• intestinal crypt stem cells show increased cell death following tamoxifen administration
• 3 weeks after tamoxifen administration, mutant villus epithelial cells show disrupted cell polarity, as indicated by disorganized nuclear alignment
• stem cells contribute less to the villus epithelial compartments than in controls following tamoxifen administration
• 3 weeks after tamoxifen administration, more mutant stem cells in intestinal crypts undergo mitosis compared to control stem cells
• stem cells contribute less to the villus epithelial compartments than in controls following tamoxifen administration, indicating reduced clonal expansion of mutant stem cells
• 2 weeks after tamoxifen administration, mutant stem cells in intestinal crypts fail to give rise to Paneth cells
• 3 weeks after tamoxifen administration, mutant villus epithelial cells show disrupted cell polarity, as indicated by disorganized nuclear alignment

endocrine/exocrine glands
• 3 weeks after tamoxifen administration, more mutant stem cells in intestinal crypts undergo mitosis compared to control stem cells
• stem cells contribute less to the villus epithelial compartments than in controls following tamoxifen administration, indicating reduced clonal expansion of mutant stem cells
• 2 weeks after tamoxifen administration, mutant stem cells in intestinal crypts fail to give rise to Paneth cells

cellular
• intestinal crypt stem cells show increased cell death following tamoxifen administration




Genotype
MGI:5308972
cn14
Allelic
Composition
Cdx2tm2Fbe/Cdx2tm2Fbe
Lgr5tm1(cre/ERT2)Cle/Lgr5+
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdx2tm2Fbe mutation (0 available); any Cdx2 mutation (16 available)
Lgr5tm1(cre/ERT2)Cle mutation (1 available); any Lgr5 mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
digestive/alimentary system
• by 6 weeks after treatment cystic vesicles persist and are often filled with mucous and detritus
• an excess of Paneth cells are found by 19-62 weeks after treatment and these cells contain an abnormally abundant number of normal looking secretory granules
• 4 weeks after treatment with tamoxifen a low level of excess mucous secretion is seen
• by 6 weeks after treatment dying Paneth cells are commonly seen

cellular
• recombined cells fail to migrate out of the crypts and up the villi in the intestine

endocrine/exocrine glands
• by 6 weeks after treatment cystic vesicles persist and are often filled with mucous and detritus
• an excess of Paneth cells are found by 19-62 weeks after treatment and these cells contain an abnormally abundant number of normal looking secretory granules




Genotype
MGI:6367565
cn15
Allelic
Composition
Gata6osem1Zfa/Gata6osem1Zfa
Lgr5tm1(cre/ERT2)Cle/Lgr5+
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gata6osem1Zfa mutation (0 available); any Gata6os mutation (0 available)
Lgr5tm1(cre/ERT2)Cle mutation (1 available); any Lgr5 mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
digestive/alimentary system
• reduced intestinal stem cells with impaired organoid formation capacity
• however, overexpression of Ehf rescues this phenotype
• short with fewer cells than in wild-type mice
• short with fewer cells than in wild-type mice
• impaired intestinal epithelium regeneration after radiation injury compared with wild-type mice

endocrine/exocrine glands
• short with fewer cells than in wild-type mice

homeostasis/metabolism
• impaired intestinal epithelium regeneration after radiation injury compared with wild-type mice




Genotype
MGI:6367579
cn16
Allelic
Composition
Bptfem1Zfa/Bptfem1Zfa
Ehfem1Zfa/Ehfem1Zfa
Lgr5tm1(cre/ERT2)Cle/Lgr5+
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bptfem1Zfa mutation (0 available); any Bptf mutation (104 available)
Ehfem1Zfa mutation (0 available); any Ehf mutation (5 available)
Lgr5tm1(cre/ERT2)Cle mutation (1 available); any Lgr5 mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
digestive/alimentary system
• almost blocked organoid formation from intestinal stem cells

homeostasis/metabolism
• severely impaired intestinal epithelium regeneration after radiation injury compared with wild-type mice




Genotype
MGI:5427869
cn17
Allelic
Composition
Cdc42tm1Brak/Cdc42tm1Brak
Lgr5tm1(cre/ERT2)Cle/Lgr5+
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdc42tm1Brak mutation (0 available); any Cdc42 mutation (35 available)
Lgr5tm1(cre/ERT2)Cle mutation (1 available); any Lgr5 mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
digestive/alimentary system
• one week after tamoxifen administration, 56% of mutant crypts show stem cells forming a clustering pattern lacking clear demarcation between each other, while at 3 weeks after tamoxifen administration, 85% do so
• stem cells in mutant crypts 3 weeks after tamoxifen treatment lose the typical triangle shapes and marker analysis indicates disrupted crypt cell polarity
• loss of stem cells
• intestinal crypt cells, most likely Paneth cells, contain large vacuoles
• reduction or absence of Paneth cell granules, indicating loss of Paneth cells

endocrine/exocrine glands
• one week after tamoxifen administration, 56% of mutant crypts show stem cells forming a clustering pattern lacking clear demarcation between each other, while at 3 weeks after tamoxifen administration, 85% do so
• stem cells in mutant crypts 3 weeks after tamoxifen treatment lose the typical triangle shapes and marker analysis indicates disrupted crypt cell polarity
• loss of stem cells
• intestinal crypt cells, most likely Paneth cells, contain large vacuoles
• reduction or absence of Paneth cell granules, indicating loss of Paneth cells




Genotype
MGI:5432137
cn18
Allelic
Composition
Apctm2.1Cip/Apc+
Lgr5tm1(cre/ERT2)Cle/Lgr5+
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apctm2.1Cip mutation (2 available); any Apc mutation (57 available)
Lgr5tm1(cre/ERT2)Cle mutation (1 available); any Lgr5 mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
neoplasm
N
• following tamoxifen treatment, no intestinal tumors are observed




Genotype
MGI:6367576
cn19
Allelic
Composition
Bptfem1Zfa/Bptfem1Zfa
Lgr5tm1(cre/ERT2)Cle/Lgr5+
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bptfem1Zfa mutation (0 available); any Bptf mutation (104 available)
Lgr5tm1(cre/ERT2)Cle mutation (1 available); any Lgr5 mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
digestive/alimentary system
• reduced intestinal stem cells with impaired organoid formation capacity




Genotype
MGI:5898004
cn20
Allelic
Composition
Elp3tm1.1Tac/Elp3tm1.1Tac
Lgr5tm1(cre/ERT2)Cle/Lgr5+
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6 * C57BL/6NTac
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Elp3tm1.1Tac mutation (0 available); any Elp3 mutation (27 available)
Lgr5tm1(cre/ERT2)Cle mutation (1 available); any Lgr5 mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
digestive/alimentary system
N
• tamoxifen treated mice show normal numbers of Lgr5+ cells in intestinal crypts




Genotype
MGI:5432135
cx21
Allelic
Composition
Lgr5tm1(cre/ERT2)Cle/Lgr5+
Lrig1tm1.1(cre/ERT2)Rjc/Lrig1tm1.1(cre/ERT2)Rjc
Genetic
Background
involves: 129 * 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lgr5tm1(cre/ERT2)Cle mutation (1 available); any Lgr5 mutation (23 available)
Lrig1tm1.1(cre/ERT2)Rjc mutation (1 available); any Lrig1 mutation (31 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
neoplasm
• at 5-6 months, nearly 90% of mice develop duodenal tumors (usually low grade adenomas)
• in one instance, a 13-month old mouse had a tumor with areas showing high grade dysplasia, and focal extension of neoplastic glands into deeper layers of the bowel wall; tumor showed intact Apc however

digestive/alimentary system
• number of crypts showing Lgr5-EGFP fluorescence is doubled in absence of Lrig1 protein, compared to mice with one or two copies of Lrig1
• at 5-6 months, nearly 90% of mice develop duodenal tumors (usually low grade adenomas)
• in one instance, a 13-month old mouse had a tumor with areas showing high grade dysplasia, and focal extension of neoplastic glands into deeper layers of the bowel wall; tumor showed intact Apc however

endocrine/exocrine glands
• number of crypts showing Lgr5-EGFP fluorescence is doubled in absence of Lrig1 protein, compared to mice with one or two copies of Lrig1





Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
01/07/2020
MGI 6.14
The Jackson Laboratory