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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Il12btm1Jm
targeted mutation 1, Jeanne Magram
MGI:1857201
Summary 10 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Il12btm1Jm/Il12btm1Jm 129S1/Sv-Il12btm1Jm MGI:3691135
hm2
 		Il12btm1Jm/Il12btm1Jm B6.129S1-Il12btm1Jm/J MGI:3691133
hm3
 		Il12btm1Jm/Il12btm1Jm involves: 129S1/Sv MGI:4459518
hm4
 		Il12btm1Jm/Il12btm1Jm involves: 129S1/SvImJ MGI:3784502
cn5
 		Il12btm1Jm/Il12btm1Jm
Stat3tm2Aki/Stat3tm2Aki
Lyz2tm1(cre)Ifo/Lyz2+ 		involves: 129P2/OlaHsd * 129S1/Sv * C57BL/6 MGI:3771395
cx6
 		Il12atm1Jm/Il12atm1Jm
Il12btm1Jm/Il12btm1Jm 		B6.129S1-Il12atm1Jm Il12btm1Jm MGI:3784444
cx7
 		Il10tm1Cgn/Il10tm1Cgn
Il12btm1Jm/Il12btm1Jm 		involves: 129P2/OlaHsd * 129S1/Sv * C57BL/6 MGI:3851989
cx8
 		Il12btm1Jm/Il12btm1Jm
Il18tm1Aki/Il18tm1Aki 		involves: 129P2/OlaHsd * 129S1/SvImJ MGI:3784501
cx9
 		Il12btm1Jm/Il12btm1Jm
Rag2tm1Fwa/Rag2tm1Fwa 		involves: 129S/SvEv * 129S1/Sv MGI:3817879
cx10
 		Il12btm1Jm/Il12btm1Jm
Tnftm2Gkl/Tnf+ 		involves: 129S/SvEv * 129S1/Sv * C57BL/6J MGI:3629590


Genotype
MGI:3691135
hm1
Allelic
Composition		 Il12btm1Jm/Il12btm1Jm
Genetic
Background		 129S1/Sv-Il12btm1Jm
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il12btm1Jm mutation (4 available); any Il12b mutation (31 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal CD4-positive, alpha-beta T cell physiology ( J:34164 )
• 75 days after infection Ifng mRNA expression in CD4+ cells is more than 50-fold lower than in wild-type
• 75 days after infection Il4 mRNA expression in CD4+ cells is 100-fold higher than in wild-type
• draining lymph node cells in L. major infected mice produce significantly more IL4 compared to IFNG in contrast to wild-type mice which express more IFNG than IL4
decreased susceptibility to type IV hypersensitivity reaction ( J:34164 )
• do not show any delayed type hypersensitivity response to application of Leishmania antigen to the uninfected footpad 41 days after initial infection with L. major
increased susceptibility to parasitic infection ( J:34164 )
• develop large lesions unlike wild-type 129S1/Sv mice after infection with Leishmania major
• 75 days after infection with L. major parasite numbers in the draining lymph nodes are increased 400-fold compared to wild-type mice

hematopoietic system
abnormal CD4-positive, alpha-beta T cell physiology ( J:34164 )
• 75 days after infection Ifng mRNA expression in CD4+ cells is more than 50-fold lower than in wild-type
• 75 days after infection Il4 mRNA expression in CD4+ cells is 100-fold higher than in wild-type
• draining lymph node cells in L. major infected mice produce significantly more IL4 compared to IFNG in contrast to wild-type mice which express more IFNG than IL4




Genotype
MGI:3691133
hm2
Allelic
Composition		 Il12btm1Jm/Il12btm1Jm
Genetic
Background		 B6.129S1-Il12btm1Jm/J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il12btm1Jm mutation (4 available); any Il12b mutation (31 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
increased susceptibility to xenobiotic induced morbidity/mortality ( J:91927 )
• increase in mortality following a single dose of N-methyl-N-nitrosourea (MNU) compared to similarly treated wild-type controls

growth/size/body
alveolar process atrophy ( J:147935 )
• 3 fold increase in alveolar bone loss at 30 weeks relative to mice at 6 and 16 weeks

immune system
abnormal granulocyte physiology ( J:111568 )
• fewer than expected granulocytes in the dermis of carcinogen treated skin
abnormal lymphocyte physiology ( J:120806 )
• lymph node cells from keyhole limpet hemocyanin (KLH) immunized mice are severely impaired in their ability to produce IFNG when cultured with KLH; however, their production of IL4 is increased
abnormal NK cell physiology ( J:120806 )
• mean NK lytic activity is about 66% of controls; however when cultured with IL2 lytic activity is similar to controls
abnormal CD8-positive, alpha-beta T cell physiology ( J:111568 )
• dramatic increase in the number of cytotoxic CD8+ cells in the dermis and epidermis of carcinogen treated skin
abnormal macrophage physiology ( J:111568 )
• fewer than expected macrophages in the dermis of carcinogen treated skin
decreased circulating interferon-gamma level ( J:120806 )
• serum levels of IFNG in LPS-injected mice are only 17 +/- 5% of LPS-induced controls
decreased susceptibility to type IV hypersensitivity reaction ( J:120806 )
• specific foot pad swelling 48 hours after challenge with methylated bovine serum albumin is inhibited by 47 +/- 3% compared to wild-type mice; however, cytotoxic T lymphocyte responses are similar to wild-type
decreased interleukin-17 secretion ( J:111568 )
• barely detectable levels of IL17 in the skin after carcinogen treatment unlike wild-type mice which express high levels of IL17
increased susceptibility to bacterial infection ( J:120806 )
• vaccination does not produce a protective response to M. tuberculosis

neoplasm
decreased incidence of tumors by chemical induction ( J:111568 )
• resistant to induction of papillomas compared to wild-type mice after treatment with DMBA and TPA in a 2 step skin carcinogenesis protocol
• however, there is an increase in the incidence of tumors following intradermal challenge with PDV squamous carcinoma cells

homeostasis/metabolism
decreased circulating interferon-gamma level ( J:120806 )
• serum levels of IFNG in LPS-injected mice are only 17 +/- 5% of LPS-induced controls
increased susceptibility to xenobiotic induced morbidity/mortality ( J:91927 )
• increase in mortality following a single dose of N-methyl-N-nitrosourea (MNU) compared to similarly treated wild-type controls
decreased incidence of tumors by chemical induction ( J:111568 )
• resistant to induction of papillomas compared to wild-type mice after treatment with DMBA and TPA in a 2 step skin carcinogenesis protocol
• however, there is an increase in the incidence of tumors following intradermal challenge with PDV squamous carcinoma cells

craniofacial
alveolar process atrophy ( J:147935 )
• 3 fold increase in alveolar bone loss at 30 weeks relative to mice at 6 and 16 weeks

skeleton
alveolar process atrophy ( J:147935 )
• 3 fold increase in alveolar bone loss at 30 weeks relative to mice at 6 and 16 weeks

hematopoietic system
abnormal granulocyte physiology ( J:111568 )
• fewer than expected granulocytes in the dermis of carcinogen treated skin
abnormal lymphocyte physiology ( J:120806 )
• lymph node cells from keyhole limpet hemocyanin (KLH) immunized mice are severely impaired in their ability to produce IFNG when cultured with KLH; however, their production of IL4 is increased
abnormal NK cell physiology ( J:120806 )
• mean NK lytic activity is about 66% of controls; however when cultured with IL2 lytic activity is similar to controls
abnormal CD8-positive, alpha-beta T cell physiology ( J:111568 )
• dramatic increase in the number of cytotoxic CD8+ cells in the dermis and epidermis of carcinogen treated skin
abnormal macrophage physiology ( J:111568 )
• fewer than expected macrophages in the dermis of carcinogen treated skin




Genotype
MGI:4459518
hm3
Allelic
Composition		 Il12btm1Jm/Il12btm1Jm
Genetic
Background		 involves: 129S1/Sv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il12btm1Jm mutation (4 available); any Il12b mutation (31 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
increased susceptibility to bacterial infection induced morbidity/mortality ( J:161073 )
• mice died before day 12 after inoculation with C. rodentium

immune system
increased susceptibility to bacterial infection induced morbidity/mortality ( J:161073 )
• mice died before day 12 after inoculation with C. rodentium




Genotype
MGI:3784502
hm4
Allelic
Composition		 Il12btm1Jm/Il12btm1Jm
Genetic
Background		 involves: 129S1/SvImJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il12btm1Jm mutation (4 available); any Il12b mutation (31 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
impaired natural killer cell mediated cytotoxicity ( J:46514 )
• the killing activity of splenic NK cells is about 2/3rds that of wild-type mice as measured by in vitro co-culturing with YAC-1 target cells
• normal level of target cell killing can be restored by in vivo pretreatment with IL-18 for two days or the addition of IL-18 to the co-culture
abnormal T-helper 1 physiology ( J:46514 )
• in vivo Th1 cell response is impaired as indicated by the low amounts of IFN-gamma produced by T cells in response to M. bovis antigens
decreased interferon-gamma secretion ( J:46514 )
• splenic T cells produce about 1/4th the amount of IFN-gamma as T cells from wild-type mice 14 days after injection with heat killed M. Bovis

hematopoietic system
impaired natural killer cell mediated cytotoxicity ( J:46514 )
• the killing activity of splenic NK cells is about 2/3rds that of wild-type mice as measured by in vitro co-culturing with YAC-1 target cells
• normal level of target cell killing can be restored by in vivo pretreatment with IL-18 for two days or the addition of IL-18 to the co-culture
abnormal T-helper 1 physiology ( J:46514 )
• in vivo Th1 cell response is impaired as indicated by the low amounts of IFN-gamma produced by T cells in response to M. bovis antigens




Genotype
MGI:3771395
cn5
Allelic
Composition		 Il12btm1Jm/Il12btm1Jm
Stat3tm2Aki/Stat3tm2Aki
Lyz2tm1(cre)Ifo/Lyz2+
Genetic
Background		 involves: 129P2/OlaHsd * 129S1/Sv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il12btm1Jm mutation (4 available); any Il12b mutation (31 available)
Lyz2tm1(cre)Ifo mutation (14 available); any Lyz2 mutation (41 available)
Stat3tm2Aki mutation (1 available); any Stat3 mutation (70 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
digestive/alimentary system
digestive/alimentary phenotype ( J:83280 )
N
• mice exhibit normal Th1 responses and do not develop colitis, unlike Stat3 null mice




Genotype
MGI:3784444
cx6
Allelic
Composition		 Il12atm1Jm/Il12atm1Jm
Il12btm1Jm/Il12btm1Jm
Genetic
Background		 B6.129S1-Il12atm1Jm Il12btm1Jm
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il12atm1Jm mutation (2 available); any Il12a mutation (33 available)
Il12btm1Jm mutation (4 available); any Il12b mutation (31 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
impaired natural killer cell mediated cytotoxicity ( J:125611 )
• following infection with Leishmania infantum

hematopoietic system
impaired natural killer cell mediated cytotoxicity ( J:125611 )
• following infection with Leishmania infantum




Genotype
MGI:3851989
cx7
Allelic
Composition		 Il10tm1Cgn/Il10tm1Cgn
Il12btm1Jm/Il12btm1Jm
Genetic
Background		 involves: 129P2/OlaHsd * 129S1/Sv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il10tm1Cgn mutation (15 available); any Il10 mutation (44 available)
Il12btm1Jm mutation (4 available); any Il12b mutation (31 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
abnormal T-helper 2 cell differentiation ( J:111712 )
• mice exhibit enhanced Th2 responses after infection with S. mansoni eggs compared to controls

immune system
abnormal T-helper 2 cell differentiation ( J:111712 )
• mice exhibit enhanced Th2 responses after infection with S. mansoni eggs compared to controls
increased interleukin-13 secretion ( J:111712 )
• there is a 200- to 250-fold increase in IL-13 mRNA present in the lungs after infection with S. mansoni eggs




Genotype
MGI:3784501
cx8
Allelic
Composition		 Il12btm1Jm/Il12btm1Jm
Il18tm1Aki/Il18tm1Aki
Genetic
Background		 involves: 129P2/OlaHsd * 129S1/SvImJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il12btm1Jm mutation (4 available); any Il12b mutation (31 available)
Il18tm1Aki mutation (3 available); any Il18 mutation (29 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
impaired natural killer cell mediated cytotoxicity ( J:46514 )
• the killing ability of splenic NK cells is severely impaired with significantly less activity occurring than NK cells from Il18tm1Aki or Il12btm1Jm homozygotes
abnormal T-helper 1 physiology ( J:46514 )
• in vivo Th1 cell response is impaired as indicated by the low amounts of IFN-gamma produced by T cells in response to M. bovis antigens
decreased interferon-gamma secretion ( J:46514 )
• splenic T cells produce 12-fold lower amounts of IFN-gamma as T cells from wild-type mice 14 days after injection with heat killed M. Bovis

hematopoietic system
impaired natural killer cell mediated cytotoxicity ( J:46514 )
• the killing ability of splenic NK cells is severely impaired with significantly less activity occurring than NK cells from Il18tm1Aki or Il12btm1Jm homozygotes
abnormal T-helper 1 physiology ( J:46514 )
• in vivo Th1 cell response is impaired as indicated by the low amounts of IFN-gamma produced by T cells in response to M. bovis antigens




Genotype
MGI:3817879
cx9
Allelic
Composition		 Il12btm1Jm/Il12btm1Jm
Rag2tm1Fwa/Rag2tm1Fwa
Genetic
Background		 involves: 129S/SvEv * 129S1/Sv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il12btm1Jm mutation (4 available); any Il12b mutation (31 available)
Rag2tm1Fwa mutation (48 available); any Rag2 mutation (117 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal chemokine secretion ( J:140483 )
• chemokines KC and MIP-2 are increased in NK cells in infected lungs
abnormal interferon secretion ( J:140483 )
• splenocytes produce minimal levels of Ifng in response to mycobacterial stimulation, in contrast to response of Rag2-null cells
lung inflammation ( J:140483 )
• at 28 days post-infection (p.i.), lungs display exacerbated pulmonary inflammatory pathology relative to Rag2-null animals; lesions contain significant numbers of granulocytes compared to Il2rg, Rag2-double null mice
increased susceptibility to bacterial infection ( J:140483 )
• mice succumb rapidly to infection compared to Rag2-null mice
• in lungs and spleens of M. tuberculosis-infected mice at 28 days post-infection (p.i.), bacterial loads are 0.5-1.0 log higher than in infected Rag2-null mice

respiratory system
lung inflammation ( J:140483 )
• at 28 days post-infection (p.i.), lungs display exacerbated pulmonary inflammatory pathology relative to Rag2-null animals; lesions contain significant numbers of granulocytes compared to Il2rg, Rag2-double null mice




Genotype
MGI:3629590
cx10
Allelic
Composition		 Il12btm1Jm/Il12btm1Jm
Tnftm2Gkl/Tnf+
Genetic
Background		 involves: 129S/SvEv * 129S1/Sv * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il12btm1Jm mutation (4 available); any Il12b mutation (31 available)
Tnftm2Gkl mutation (1 available); any Tnf mutation (46 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
small intestinal inflammation ( J:108572 )
• mice display delayed development and attenuation of inflammatory bowel disease

digestive/alimentary system
small intestinal inflammation ( J:108572 )
• mice display delayed development and attenuation of inflammatory bowel disease




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Send questions and comments to User Support. 		last database update
04/30/2024
MGI 6.23 		The Jackson Laboratory