About   Help   FAQ
Targeted Allele Detail
Symbol: Tnftm2Gkl
Name: tumor necrosis factor; targeted mutation 2, George Kollias
MGI ID: MGI:2388715
Synonyms: TNFdeltaARE
Gene: Tnf  Location: Chr17:35418357-35420983 bp, - strand  Genetic Position: Chr17, 18.59 cM
Alliance: Tnftm2Gkl page
Germline Transmission:  Earliest citation of germline transmission: J:54056
Parent Cell Line:  CCE/EK.CCE (ES Cell)
Strain of Origin:  129S/SvEv-Gpi1c
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation details69 bp encompassing the AU-rich elements (ARE) were deleted by homologous recombination of a floxed neo cassette and subsequent in vitro cre mediated excision. The resultant allele contained a single loxP site in place of the ARE sequence. ELISA of homozygous mutant mice showed protein levels higher than in wild-type mice, indicating a suppressive role of the AREs. (J:54056)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
In Structures Affected by this Mutation: 14 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Tnf Mutation:  46 strains or lines available
Original:  J:54056 Kontoyiannis D, et al., Impaired on/off regulation of TNF biosynthesis in mice lacking TNF AU- rich elements: implications for joint and gut-associated immunopathologies. Immunity. 1999 Mar;10(3):387-98
All:  48 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
MGI 6.23
The Jackson Laboratory