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Gene Expression Data
Assay Details
Assay
Reference: J:209428 Ragancokova D, et al., TSHZ1-dependent gene regulation is essential for olfactory bulb development and olfaction. J Clin Invest. 2014 Mar 3;124(3):1214-27
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7491839
Gene symbol: Tshz1
Gene name: teashirt zinc finger family member 1
Reporter: GFP
Antibody: anti-GFP
Results
Specimen S1E control: embryonic day 18.5; Tshz1tm1Garr/Tshz1tm2.1Garr (more )
Note: Double labeled: green - Tshz1 reporter; red - Tshz2 (assay MGI:7491891).
Structure Level Pattern Image Note
TS26: main olfactory bulb prospective granule cell layer Present Regionally restricted S1E control Expression in ring of cells in outer granule cell layer directly abutting mitral cell layer.
TS26: rostral migratory stream Present Scattered S1E control Expression in scattered cells unevenly distributed throughout rostral migratory stream/subventricular zone of olfactory bulb.
TS26: main olfactory bulb mitral cell layer Present S1E control Co-expression with Tshz2 in mitral cells.
Specimen S1E Tshz1-/-: embryonic day 18.5; Tshz1tm1Garr/Tshz1tm2.2Garr (more )
Note: Double labeled: green - Tshz1 reporter; red - Tshz2 (assay MGI:7491891).
Structure Level Pattern Image Note
TS26: main olfactory bulb prospective granule cell layer Present Scattered S1E Tshz1-/- Expression in scattered cells as well as aggregates located mainly within inner granule cell layer/subventricular zone of olfactory bulb.
TS26: main olfactory bulb mitral cell layer Present Scattered S1E Tshz1-/- Co-expression with Tshz2 in mitral cells.
Specimen S1F control: embryonic day 18.5; Tshz1tm1Garr/Tshz1tm2.1Garr (more )
Note: Double labeled: green - Tshz1 reporter; red - Tshz2 (assay MGI:7491891).
Structure Level Pattern Image Note
TS26: main olfactory bulb prospective granule cell layer Present Regionally restricted S1F control Ring of expression in outer granule cell layer directly abutting mitral cell layer.
TS26: rostral migratory stream Present Scattered S1F control Expression in scattered cells throughout rostral migratory stream/subventricular zone of olfactory bulb.
TS26: main olfactory bulb mitral cell layer Present S1F control Co-expression with Tshz2 in mitral cells.
Specimen S1F coTshz1: embryonic day 18.5; Tshz1tm1Garr/Tshz1tm2.1Garr, Tg(Nes-cre)1Kln/? (more )
Note: Conditional mutant. Double labeled: green - Tshz1 reporter; red - Tshz2 (assay MGI:7491891).
Structure Level Pattern Image Note
TS26: main olfactory bulb prospective granule cell layer Present Scattered S1F coTshz1 Expression in scattered cells as well as aggregates located mainly within inner granule cell layer/subventricular zone of olfactory bulb.
TS26: main olfactory bulb mitral cell layer Present S1F coTshz1 Co-expression with Tshz2 in mitral cells.
Specimen 2C/D Control: postnatal day 0.5; Tshz1tm1Garr/Tshz1tm2.1Garr (more )
Note: Double labeled: green - Tshz1 reporter; red - Rbfox3 (assay MGI:7491890).
Structure Level Pattern Image Note
TS27: main olfactory bulb granule cell layer Present Regionally restricted 2D Control inset , 2C Control , 2D Control Expression in outer granule cell layer.
Specimen 2C/D coTshz1: postnatal day 0.5; Tshz1tm1Garr/Tshz1tm2.1Garr, Tg(Nes-cre)1Kln/? (more )
Note: Conditional mutant. Double labeled: green - Tshz1 reporter; red - Rbfox3 (assay MGI:7491890).
Structure Level Pattern Image Note
TS27: main olfactory bulb Present Regionally restricted 2D coTshz1 inset , 2D coTshz1 , 2C coTshz1 Expression in aggregates.
Specimen 2H/I Control: postnatal day 21; Tshz1tm1Garr/Tshz1tm2.1Garr (more )
Note: Double labeled: green - Tshz1 reporter; red - Rbfox3 (assay MGI:7491890).
Structure Level Pattern Image Note
TS28: rostral migratory stream Weak 2I Control , 2H Control Weak expression in rostral migratory stream.
TS28: main olfactory bulb external plexiform layer Strong Regionally restricted 2I Control , 2H Control Stronger expression throughout differentiated NeuN+ cells of granule cell and external plexiform layers.
TS28: main olfactory bulb granule cell layer Strong Regionally restricted 2I Control , 2H Control Stronger expression throughout differentiated NeuN+ cells of granule cell and external plexiform layers.
TS28: main olfactory bulb glomerular layer Present Regionally restricted 2H Control , 2I Control Expression in a subpopulation of periglomerular neurons.
Specimen 2H/I coTshz1: postnatal day 21; Tshz1tm1Garr/Tshz1tm2.1Garr, Tg(Nes-cre)1Kln/? (more )
Note: Conditional mutant. Double labeled: green - Tshz1 reporter; red - Rbfox3 (assay MGI:7491890).
Structure Level Pattern Image Note
TS28: rostral migratory stream Strong 2I coTshz1 , 2H coTshz1 Expression in rostral migratory stream much more prominent than in control.
TS28: main olfactory bulb Weak Regionally restricted 2H coTshz1 , 2I coTshz1 Substantially reduced expression in differentiated neurons surrounding rostral migratory stream and extending up to glomerular layer.
Specimen 3C RMSob Control: postnatal adult; Tshz1tm1Garr/Tshz1tm2.1Garr (more )
Note: Double labeled: green - Tshz1 reporter; red - Dcx (assay MGI:7491892).
Structure Level Pattern Image Note
TS28: rostral migratory stream Weak 3C RMS Control
Specimen 3C GCL Control: postnatal adult; Tshz1tm1Garr/Tshz1tm2.1Garr (more )
Note: Double labeled: green - Tshz1 reporter; red - Dcx (assay MGI:7491892).
Structure Level Pattern Image Note
TS28: main olfactory bulb granule cell layer Present 3C GCL Control
Specimen 3C RMSob coTshz1: postnatal adult; Tshz1tm1Garr/Tshz1tm2.1Garr, Tg(Nes-cre)1Kln/? (more )
Note: Conditional mutant. Double labeled: green - Tshz1 reporter; red - Dcx (assay MGI:7491892).
Structure Level Pattern Image Note
TS28: rostral migratory stream Present 3C RMS coTshz1
Specimen 3C GCL coTshz1: postnatal adult; Tshz1tm1Garr/Tshz1tm2.1Garr, Tg(Nes-cre)1Kln/? (more )
Note: Conditional mutant. Double labeled: green - Tshz1 reporter; red - Dcx (assay MGI:7491892).
Structure Level Pattern Image Note
TS28: main olfactory bulb granule cell layer Present 3C GCL coTshz1
Specimen 5B Control: postnatal; Tshz1tm1Garr/Tshz1tm2.1Garr (more )
Note: Double labeled: green - Tshz1 reporter; red - Th (assay MGI:7491893).
Structure Level Pattern Image Note
TS28: main olfactory bulb glomerular layer Present 5B Control
Specimen 5B coTshz1: postnatal; Tshz1tm1Garr/Tshz1tm2.1Garr, Tg(Nes-cre)1Kln/? (more )
Note: Conditional mutant. Double labeled: green - Tshz1 reporter; red - Th (assay MGI:7491893).
Structure Level Pattern Image Note
TS28: main olfactory bulb glomerular layer Weak 5B coTshz1 Fewer expressing cells surrounding outer parts of glomeruli.
Specimen 5D Control: postnatal; Tshz1tm1Garr/Tshz1tm2.1Garr (more )
Note: Double labeled: green - Tshz1 reporter; red - Calb2 (assay MGI:7491894).
Structure Level Pattern Image Note
TS28: main olfactory bulb glomerular layer Present 5D Control
Specimen 5D coTshz1: postnatal; Tshz1tm1Garr/Tshz1tm2.1Garr, Tg(Nes-cre)1Kln/? (more )
Note: Conditional mutant. Double labeled: green - Tshz1 reporter; red - Calb2 (assay MGI:7491894).
Structure Level Pattern Image Note
TS28: main olfactory bulb glomerular layer Weak Regionally restricted 5D coTshz1 Fewer expressing cells surrounding outer parts of glomeruli.
Specimen 5F Control: postnatal; Tshz1tm1Garr/Tshz1tm2.1Garr (more )
Note: Double labeled: green - Tshz1 reporter; red - Calb1 (assay MGI:7491895).
Structure Level Pattern Image Note
TS28: main olfactory bulb glomerular layer Present 5F Control
Specimen 5F coTshz1: postnatal; Tshz1tm1Garr/Tshz1tm2.1Garr, Tg(Nes-cre)1Kln/? (more )
Note: Conditional mutant. Double labeled: green - Tshz1 reporter; red - Calb1 (assay MGI:7491895).
Structure Level Pattern Image Note
TS28: main olfactory bulb glomerular layer Weak Regionally restricted 5F coTshz1 Fewer expressing cells surrounding outer parts of glomeruli.
Specimen S6A control: postnatal; Tshz1tm1Garr/Tshz1tm2.1Garr (more )
Note: Double labeled: green - Tshz1 reporter; red - Pax6 (assay MGI:7491896).
Structure Level Pattern Image Note
TS28: main olfactory bulb glomerular layer Present S6A control GFP/PAX6 , S6A control (inset) Expression within glomerular layer in population of periglomerular neurons, non-overlapping with Pax6 expression.
Specimen S6A coTshz1: postnatal; Tshz1tm1Garr/Tshz1tm2.1Garr, Tg(Nes-cre)1Kln/? (more )
Note: Conditional mutant. Double labeled: green - Tshz1 reporter; red - Pax6 (assay MGI:7491896).
Structure Level Pattern Image Note
TS28: main olfactory bulb glomerular layer Weak Regionally restricted S6B coTshz1 GFP/PAX6 , S6B coTshz1 (inset)) Reduced number of expressing cells around glomeruli, with many clustering on inner side of glomeruli apposing external plexiform layer. Some co-expression with Pax6.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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