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Gene Expression Data
Assay Details
Assay
Reference: J:207609 Hilliard SA, et al., Mdm2 is required for maintenance of the nephrogenic niche. Dev Biol. 2014 Mar 1;387(1):1-14
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7481327
Gene symbol: Six2
Gene name: sine oculis-related homeobox 2
Antibody: anti-Six2 (11562-1-AP)
Detection system: Secondary antibody coupled to Alexa Fluor 647
Results
Specimen 2I: embryonic day 14.5 (more )
Note: Double labeled: red - Six2; green - Cdh1 (assay MGI:7481328).
Structure Level Pattern Image Note
TS22: cap mesenchyme Present 2I Positive cap mesenchyme was several layers thick and more robust.
Specimen 2J: embryonic day 14.5; Mdm2tm1.2Mep/Mdm2tm1.2Mep, Tg(Six2-GFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: red - Six2; green - Cdh1 (assay MGI:7481328).
Structure Level Pattern Image Note
TS22: cap mesenchyme Present 2J A four-fold reduction in the positive nephron progenitor cells compared to wild type. The few surviving positive cap cells formed a thin layer on the dorsal aspect of the ureteric bud tips, failing to extend more ventrally.
Specimen 2K: embryonic day 16.5 (more )
Note: Double labeled: red - Six2; green - Cdh1 (assay MGI:7481328).
Structure Level Pattern Image Note
TS24: cap mesenchyme Present 2K Positive cap mesenchyme was several layers thick and more robust.
Specimen 2L: embryonic day 16.5; Mdm2tm1.2Mep/Mdm2tm1.2Mep, Tg(Six2-GFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: red - Six2; green - Cdh1 (assay MGI:7481328).
Structure Level Pattern Image Note
TS24: cap mesenchyme Present 2L A thinner cap, reduced to 1-2 cell layers in thickness, was seen in the mutant.
Specimen 3A: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: cap mesenchyme Present 3A
Specimen 3B: embryonic day 14.5; Mdm2tm1.2Mep/Mdm2tm1.2Mep, Tg(Six2-GFP/cre)1Amc/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS22: cap mesenchyme Present 3B A significant loss of the Six2+Cited1+ nephron progenitor cells.
Specimen 4E: embryonic day 14.5 (more )
Note: Double labeled: red - Six2; green - H2ax (assay MGI:7481339).
Structure Level Pattern Image Note
TS22: cap mesenchyme Present 4E Not coexpressed with phos-H2ax.
Specimen 4F: embryonic day 14.5; Mdm2tm1.2Mep/Mdm2tm1.2Mep, Tg(Six2-GFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: red - Six2; green - H2ax (assay MGI:7481339).
Structure Level Pattern Image Note
TS22: cap mesenchyme Present 4F There were cells coexpressed with phos-H2ax (sustained DNA damage) in the mutant.
Specimen 4G: embryonic day 14.5 (more )
Note: Double labeled: red - Six2; green - H2ax (assay MGI:7481339).
Structure Level Pattern Image Note
TS22: cap mesenchyme Present 4G Not coexpressed with phos-H2ax.
Specimen 4H: embryonic day 14.5; Mdm2tm1.2Mep/Mdm2tm1.2Mep, Tg(Six2-GFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: red - Six2; green - H2ax (assay MGI:7481339).
Structure Level Pattern Image Note
TS22: cap mesenchyme Present 4H There were cells coexpressed with phos-H2ax (sustained DNA damage) in the mutant.
Specimen 5A': embryonic day 14.5 (more )
Note: Double labeled: red - Six2; green - TUNEL.
Structure Level Pattern Image Note
TS22: cap mesenchyme Present 5A' Positive cells seldom showed apoptotic foci.
Specimen 5B': embryonic day 14.5; Mdm2tm1.2Mep/Mdm2tm1.2Mep, Tg(Six2-GFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: red - Six2; green - TUNEL.
Structure Level Pattern Image Note
TS22: cap mesenchyme Present 5B' Numerous apoptotic foci were seen (quantified in Fig. 5C).
Specimen 6A/C: embryonic day 14.5 (more )
Note: Double labeled: red - Six2; green - pH3.
Structure Level Pattern Image Note
TS22: cap mesenchyme Present 6A , 6C
Specimen 6B/D: embryonic day 14.5; Mdm2tm1.2Mep/Mdm2tm1.2Mep, Tg(Six2-GFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: red - Six2; green - pH3.
Structure Level Pattern Image Note
TS22: cap mesenchyme Present 6B , 6D A significant loss of Six2 cells. The combined pHH3- and Six2-positive foci when normalized to Six2-positive cells revealed no change in mitotic rates between the wild-type control and mutant kidney.
Specimen 9A: embryonic day 14.5 (more )
Note: Double labeled: red - Six2; green - Pcna (assay MGI:7481346).
Structure Level Pattern Image Note
TS22: cap mesenchyme Present 9A
Specimen 9B: embryonic day 14.5; Mdm2tm1.2Mep/Mdm2tm1.2Mep, Tg(Six2-GFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: red - Six2; green - Pcna (assay MGI:7481346).
Structure Level Pattern Image Note
TS22: cap mesenchyme Present 9B A significant loss of Six2 cells.
Specimen 9C: embryonic day 14.5 (more )
Note: Double labeled: red - Six2; green - Pcna (assay MGI:7481346).
Structure Level Pattern Image Note
TS22: cap mesenchyme Present 9C
Specimen 9D: embryonic day 14.5; Mdm2tm1.2Mep/Mdm2tm1.2Mep, Tg(Six2-GFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: red - Six2; green - Pcna (assay MGI:7481346).
Structure Level Pattern Image Note
TS22: cap mesenchyme Present 9D A significant loss of Six2 cells.
Specimen 9E: embryonic day 14.5 (more )
Note: Double labeled: red - Six2; green - Meis1 (assay MGI:7481347).
Structure Level Pattern Image Note
TS22: cap mesenchyme Present 9E
Specimen 9F: embryonic day 14.5; Mdm2tm1.2Mep/Mdm2tm1.2Mep, Tg(Six2-GFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: red - Six2; green - Meis1 (assay MGI:7481347).
Structure Level Pattern Image Note
TS22: cap mesenchyme Present 9F A significant loss of Six2 cells.
Specimen 11A: postnatal day 0 (more )
Note: Double labeled: red - Six2 protein; green - GFP (from Cre transgene)
Structure Level Pattern Image Note
TS27: cap mesenchyme Present 11A Positive cap mesenchyme was several layers thick and more robust.
Specimen 11B: postnatal day 0; Mdm2tm1.2Mep/Mdm2tm1.2Mep, Tg(Six2-GFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: red - Six2 protein; green - GFP (from Cre transgene)
Structure Level Pattern Image Note
TS27: cap mesenchyme Present 11B A thinner cap, reduced to 1-2 cell layers in thickness, was seen in the mutant.
Specimen 11C: postnatal day 0; Mdm2tm1.2Mep/Mdm2tm1.2Mep, Tg(Six2-GFP/cre)1Amc/0, Trp53tm1Tyj/Trp53tm1Brn (more )
Note: Conditional mutant. Double labeled: red - Six2 protein; green - GFP (from Cre transgene)
Structure Level Pattern Image Note
TS27: cap mesenchyme Present 11C Six2 and GFP co-staining underscored the recovery of cap mesenchyme cells.
Specimen 11G: postnatal day 0 (more )
Note: Double labeled: red - Six2; green - Meis1 (assay MGI:7481347).
Structure Level Pattern Image Note
TS27: cap mesenchyme Present 11G Positive cap mesenchyme was several layers thick and more robust.
Specimen 11H: postnatal day 0; Mdm2tm1.2Mep/Mdm2tm1.2Mep, Tg(Six2-GFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: red - Six2; green - Meis1 (assay MGI:7481347).
Structure Level Pattern Image Note
TS27: cap mesenchyme Present 11H A thinner cap, reduced to 1-2 cell layers in thickness, was seen in the mutant.
Specimen 11I: postnatal day 0; Mdm2tm1.2Mep/Mdm2tm1.2Mep, Tg(Six2-GFP/cre)1Amc/0, Trp53tm1Tyj/Trp53tm1Brn (more )
Note: Conditional mutant. Double labeled: red - Six2; green - Meis1 (assay MGI:7481347).
Structure Level Pattern Image Note
TS27: cap mesenchyme Present 11I Recovery of cap mesenchyme cells was seen.
Specimen S2A: embryonic day 11.75 (more )
Note: Double labeled: red - Six2; green - pan cytokeratin.
Structure Level Pattern Image Note
TS20: cap mesenchyme Present S2A
Specimen S2B: embryonic day 11.75; Mdm2tm1.2Mep/Mdm2tm1.2Mep, Tg(Six2-GFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: red - Six2; green - pan cytokeratin.
Structure Level Pattern Image Note
TS20: cap mesenchyme Present S2B Smaller cap mesenchyme compared with the control.
Specimen S2C: embryonic day 12.5 (more )
Note: Double labeled: red - Six2; green - pan cytokeratin.
Structure Level Pattern Image Note
TS20: cap mesenchyme Present S2C
Specimen S2D: embryonic day 12.5; Mdm2tm1.2Mep/Mdm2tm1.2Mep, Tg(Six2-GFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: red - Six2; green - pan cytokeratin.
Structure Level Pattern Image Note
TS20: cap mesenchyme Present S2D Smaller cap mesenchyme compared with the control.
Specimen S3A: embryonic day 14.5 (more )
Note: Double labeled: red - Six2; green - Parp1 (assay MGI:7481919).
Structure Level Pattern Image Note
TS22: cap mesenchyme Present S3A Positive cells seldom coexpressed with cleaved Parp1.
Specimen S3B: embryonic day 14.5; Mdm2tm1.2Mep/Mdm2tm1.2Mep, Tg(Six2-GFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: red - Six2; green - Parp1 (assay MGI:7481919).
Structure Level Pattern Image Note
TS22: cap mesenchyme Present S3B There were cells coexpressed with cleaved Parp1 in the mutant.
Specimen not shown: embryonic day 13.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Structure Level Pattern Image Note
TS21: cap mesenchyme Present
Specimen not shown: embryonic day 13.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Mdm2tm1.2Mep/Mdm2tm1.2Mep, Tg(Six2-GFP/cre)1Amc/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: cap mesenchyme Present Six2+ cells did not undergo a fate change into stromal cells.
Specimen not shown: postnatal day 0; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Structure Level Pattern Image Note
TS27: cap mesenchyme Present
Specimen not shown: postnatal day 0; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Mdm2tm1.2Mep/Mdm2tm1.2Mep, Tg(Six2-GFP/cre)1Amc/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS27: cap mesenchyme Present Six2+ cells did not undergo a fate change into stromal cells.
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04/16/2024
MGI 6.23 		The Jackson Laboratory