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Gene Expression Data
Assay Details
Assay
Reference: J:321982 De A, et al., The beta8 integrin cytoplasmic domain activates extracellular matrix adhesion to promote brain neurovascular development. Development. 2022 Mar 15;149(6):dev200472
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7281813
Gene symbol: Aif1
Gene name: allograft inflammatory factor 1
Antibody: anti-Iba1 (019-19741)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: The antibody used in this assay was not specified; two antibodies were listed: MGI:5620494 and MGI:7281799.
Results
Specimen 3L: embryonic day 14.5 (more )
Note: Double labeled: green - Aif1; red - Pecam1 (assay MGI:7281812).
Structure Cell Type Level Pattern Image Note
TS22: midbrain microglial cell Present 3L
Specimen 3M: embryonic day 14.5; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: green - Aif1; red - Pecam1 (assay MGI:7281812).
Structure Cell Type Level Pattern Image Note
TS22: midbrain microglial cell Present 3M The Pecam+ blood vessels have abnormal morphologies and show increased juxtaposition with Aif1+ microglia.
Specimen 3N: embryonic day 14.5; Itgb8tm1Lfr/Itgb8tm1Lfr (more )
Note: Double labeled: green - Aif1; red - Pecam1 (assay MGI:7281812).
Structure Cell Type Level Pattern Image Note
TS22: midbrain microglial cell Present 3N The Pecam+ blood vessels have abnormal morphologies and show increased juxtaposition with Aif1+ microglia.
Specimen S5D cortex: embryonic day 18.5; Tg(Nes-cre)1Kln/0 (more )
Note: Double labeled: green - Aif1; red - Pecam1 (assay MGI:7281812).
Structure Cell Type Level Pattern Image Note
TS26: cerebral cortex microglial cell Present S5D Cortex
Specimen S5D midbrain: embryonic day 18.5; Tg(Nes-cre)1Kln/0 (more )
Note: Double labeled: green - Aif1; red - Pecam1 (assay MGI:7281812).
Structure Cell Type Level Pattern Image Note
TS26: midbrain microglial cell Present S5D Midbrain
Specimen S5E cortex: embryonic day 18.5; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: green - Aif1; red - Pecam1 (assay MGI:7281812).
Structure Cell Type Level Pattern Image Note
TS26: cerebral cortex microglial cell Present S5E Cortex Pecam+ cerebral blood vessels were associated with increased numbers of Aif1+ microglia.
Specimen S5E midbrain: embryonic day 18.5; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: green - Aif1; red - Pecam1 (assay MGI:7281812).
Structure Cell Type Level Pattern Image Note
TS26: midbrain microglial cell Present S5E Midbrain
Specimen S5F cortex: embryonic day 18.5; Itgb8tm1Lfr/Itgb8tm1Lfr (more )
Note: Double labeled: green - Aif1; red - Pecam1 (assay MGI:7281812).
Structure Cell Type Level Pattern Image Note
TS26: cerebral cortex microglial cell Present S5F Cortex
Specimen S5F midbrain: embryonic day 18.5; Itgb8tm1Lfr/Itgb8tm1Lfr (more )
Note: Double labeled: green - Aif1; red - Pecam1 (assay MGI:7281812).
Structure Cell Type Level Pattern Image Note
TS26: midbrain microglial cell Present S5F Midbrain
Specimen S9A lower panel: postnatal day 10; Tg(Nes-cre)1Kln/0 (more )
Note: Double labeled: green - Aif1; red - Gfap (assay MGI:7282038).
Structure Cell Type Level Pattern Image Note
TS28: cerebral cortex microglial cell Present S9A lower panel
Specimen S9B lower panel: postnatal day 10; Tg(Nes-cre)1Kln/0 (more )
Note: Double labeled: green - Aif1; red - Gfap (assay MGI:7282038).
Structure Cell Type Level Pattern Image Note
TS28: corpus striatum microglial cell Present S9B lower panel
Specimen S9C lower panel: postnatal day 10; Tg(Nes-cre)1Kln/0 (more )
Note: Double labeled: green - Aif1; red - Gfap (assay MGI:7282038).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum microglial cell Present S9C lower panel
Specimen S9D lower panel: postnatal day 10; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: green - Aif1; red - Gfap (assay MGI:7282038).
Structure Cell Type Level Pattern Image Note
TS28: cerebral cortex microglial cell Present S9D lower panel No differences in Aif1+ microglial cell numbers were noted in the mutant.
Specimen S9E lower panel: postnatal day 10; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: green - Aif1; red - Gfap (assay MGI:7282038).
Structure Cell Type Level Pattern Image Note
TS28: corpus striatum microglial cell Present S9E lower panel No differences in Aif1+ microglial cell numbers were noted in the mutant.
Specimen S9F lower panel: postnatal day 10; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: green - Aif1; red - Gfap (assay MGI:7282038).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum microglial cell Present S9F lower panel No differences in Aif1+ microglial cell numbers were noted in the mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory