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Gene Expression Data
Assay Details
Assay
Reference: J:236149 Edwards W, et al., Identification of a novel progenitor cell marker, grainyhead-like 2 in the developing pituitary. Dev Dyn. 2016 Nov;245(11):1097-1106
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6155596
Gene symbol: Grhl2
Gene name: grainyhead like transcription factor 2
Antibody: anti-GRHL2
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to Cy3
Assay notes: A tyramide signal amplification was used. Formaldehyde fixative was 3.7% or a Zinc fixative was used.
Results
Specimen 1A: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: pituitary gland Present 1A Expression is in the vast majority of cells.
Specimen 1B: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: pituitary gland Present 1B Expression is in the vast majority of cells.
Specimen 1C: postnatal day 1 (more )
Structure Level Pattern Image Note
TS27: pituitary gland Present Regionally restricted 1C Expression is restricted to the marginal cells lining the lumen.
TS27: adenohypophysis Present Single cells 1C Expression is scattered.
TS27: adenohypophysis pars intermedia Present Single cells 1C Expression is scattered in the dorsal intermediate lobe.
Specimen 1D: postnatal day 5 (more )
Structure Level Pattern Image Note
TS28: adenohypophysis Weak Regionally restricted 1D Expression is in lining the cleft.
TS28: adenohypophysis pars intermedia Weak Regionally restricted 1D Expression is in lining the cleft.
Specimen 1E: postnatal day 30 (more )
Structure Level Pattern Image Note
TS28: adenohypophysis Weak 1E Expression is lining the cleft.
TS28: adenohypophysis pars intermedia Weak 1E Expression is lining the cleft.
Specimen 2A: postnatal day 1 (more )
Note: Double labeled: magenta - Grhl2; green - Sox2 (assay MGI:6155656).
Structure Level Pattern Image Note
TS27: adenohypophysis pars intermedia Present 2C , 2A
Specimen 2D: postnatal day 1 (more )
Note: Double labeled: magenta - Grhl2; green - Sox2 (assay MGI:6155656).
Structure Level Pattern Image Note
TS27: adenohypophysis Present Regionally restricted 2F , 2D Expression is in the cleft cells.
Specimen 2G: postnatal day 1 (more )
Note: Double labeled: magenta - Grhl2; green - Sox2 (assay MGI:6155656).
Structure Level Pattern Image Note
TS27: adenohypophysis Present Single cells 2I , 2G Expression is in the parenchyma.
TS27: adenohypophysis pars intermedia Present Regionally restricted 2G Expression is in a few cells of the dorsal intermediate lobe.
Specimen 2J: postnatal day 1 (more )
Note: Double labeled: magenta - Grhl2; green - Pomc (assay MGI:6155742).
Structure Level Pattern Image Note
TS27: adenohypophysis Present Single cells 2L , 2J Expression is in the parenchyma.
TS27: adenohypophysis pars intermedia Present Single cells 2J , 2L Expression is in a few cells of the dorsal intermediate lobe.
Specimen 2M: postnatal day 1 (more )
Note: Double labeled: magenta - Grhl2; green - Pomc (assay MGI:6155742).
Structure Level Pattern Image Note
TS27: adenohypophysis pars intermedia Present 2O'' , 2M , 2O , 2O'
Specimen 2P: postnatal day 1 (more )
Note: Double labeled: magenta - Grhl2; green - Pou1f1 (assay MGI:6155745).
Structure Level Pattern Image Note
TS27: adenohypophysis Present Single cells 2P , 2R Expression is in the parenchyma.
TS27: adenohypophysis pars intermedia Present Regionally restricted 2R , 2P Expression is in a few cells of the dorsal intermediate lobe.
Specimen 2S: postnatal day 1 (more )
Note: Double labeled: magenta - Grhl2; green - Lhb (assay MGI:6155751).
Structure Level Pattern Image Note
TS27: adenohypophysis Present Regionally restricted 2S , 2U Expression is in the cleft cells.
Specimen 3A: postnatal day 5 (more )
Structure Level Pattern Image Note
TS28: adenohypophysis Present Regionally restricted 3A Expression is mainly in the cleft cells.
TS28: adenohypophysis pars intermedia Present Regionally restricted 3A Expression is mainly in the cleft cells.
Specimen 3B: postnatal day 5; Prop1df/Prop1df (more )
Structure Level Pattern Image Note
TS28: adenohypophysis Strong Regionally restricted 3B Expression at the cleft has increased in this mutant.
Specimen 3C: postnatal day 5 (more )
Structure Level Pattern Image Note
TS28: adenohypophysis Present Regionally restricted 3C Expression is mainly in the cleft cells.
TS28: adenohypophysis pars intermedia Present Regionally restricted 3C Expression is mainly in the cleft cells.
Specimen 3D: postnatal day 5; Prop1df/Prop1df (more )
Structure Level Pattern Image Note
TS28: adenohypophysis Strong Regionally restricted 3D Expression at the cleft has increased in this mutant.
Specimen 4A: postnatal day 1 (more )
Structure Level Pattern Image Note
TS27: adenohypophysis pars intermedia Present Regionally restricted 4B , 4A Expression is in the cleft cells.
TS27: adenohypophysis Present Regionally restricted 4A , 4C Expression is in the cleft cells and scattered in the parenchyma.
Specimen 4D: postnatal day 1; Foxg1tm1(cre)Skm/Foxg1+, Notch2tm3Grid/Notch2tm3Grid (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS27: adenohypophysis pars intermedia Weak Regionally restricted 4D , 4E Expression is in the cleft cells.
TS27: adenohypophysis Present Regionally restricted 4D , 4F Expression in the cleft cells is the same as in wild-type.
Specimen 6B: postnatal day 1 (more )
Note: Double labeled: red - Grhl2; green - Cdh1 (assay MGI:6156393).
Structure Level Pattern Image Note
TS27: adenohypophysis Present Regionally restricted 6B Expression is in the cleft cells.
TS27: adenohypophysis pars intermedia Present Regionally restricted 6B Expression is in the cleft cells.
Specimen 6D: postnatal day 1 (more )
Note: Double labeled: red - Grhl2; green - Cdh1 (assay MGI:6156393).
Structure Level Pattern Image Note
TS27: adenohypophysis pars intermedia Present Regionally restricted 6D Expression is in the cleft cells.
Specimen 6F: postnatal day 1 (more )
Note: Double labeled: red - Grhl2; green - Cdh1 (assay MGI:6156393).
Structure Level Pattern Image Note
TS27: adenohypophysis Present Regionally restricted 6F Expression is in the cleft cells.
Specimen 6H: postnatal day 1; Foxg1tm1(cre)Skm/Foxg1+, Notch2tm3Grid/Notch2tm3Grid (more )
Note: Conditional mutant. Double labeled: red - Grhl2; green - Cdh1 (assay MGI:6156393).
Structure Level Pattern Image Note
TS27: adenohypophysis Present Regionally restricted 6H Expression in the cleft cells is the same as in wild-type.
TS27: adenohypophysis pars intermedia Weak Regionally restricted 6H Expression is in the cleft cells.
Specimen 6J: postnatal day 1; Foxg1tm1(cre)Skm/Foxg1+, Notch2tm3Grid/Notch2tm3Grid (more )
Note: Conditional mutant. Double labeled: red - Grhl2; green - Cdh1 (assay MGI:6156393).
Structure Level Pattern Image Note
TS27: adenohypophysis pars intermedia Weak Regionally restricted 6J Expression is in the cleft cells.
Specimen 6L: postnatal day 1; Foxg1tm1(cre)Skm/Foxg1+, Notch2tm3Grid/Notch2tm3Grid (more )
Note: Conditional mutant. Double labeled: red - Grhl2; green - Cdh1 (assay MGI:6156393).
Structure Level Pattern Image Note
TS27: adenohypophysis Present Regionally restricted 6L Expression in the cleft cells is the same as in wild-type.
Specimen 7B: postnatal day 1 (more )
Note: Double labeled: red - Grhl2; green - Pcna (assay MGI:6156396).
Structure Level Pattern Image Note
TS27: adenohypophysis pars intermedia Present Regionally restricted 7B Expression is in the cleft cells.
TS27: adenohypophysis Present Regionally restricted 7B Expression is in the cleft cells and scattered in the parenchyma.
Specimen 7D: postnatal day 1 (more )
Note: Double labeled: red - Grhl2; green - Pcna (assay MGI:6156396).
Structure Level Pattern Image Note
TS27: adenohypophysis pars intermedia Present Regionally restricted 7D Expression is in the cleft cells.
Specimen 7F: postnatal day 1 (more )
Note: Double labeled: red - Grhl2; green - Pcna (assay MGI:6156396).
Structure Level Pattern Image Note
TS27: adenohypophysis Present Regionally restricted 7F Expression is in the cleft cells and scattered in the parenchyma.
Specimen 7H: postnatal day 1; Foxg1tm1(cre)Skm/Foxg1+, Notch2tm3Grid/Notch2tm3Grid (more )
Note: Conditional mutant. Double labeled: red - Grhl2; green - Pcna (assay MGI:6156396).
Structure Level Pattern Image Note
TS27: adenohypophysis pars intermedia Weak Regionally restricted 7H Expression is in the cleft cells.
TS27: adenohypophysis Present Regionally restricted 7H Expression in the cleft cells is the same as in wild-type.
Specimen 7J: postnatal day 1; Foxg1tm1(cre)Skm/Foxg1+, Notch2tm3Grid/Notch2tm3Grid (more )
Note: Conditional mutant. Double labeled: red - Grhl2; green - Pcna (assay MGI:6156396).
Structure Level Pattern Image Note
TS27: adenohypophysis pars intermedia Weak Regionally restricted 7J Expression is in the cleft cells.
Specimen 7L: postnatal day 1; Foxg1tm1(cre)Skm/Foxg1+, Notch2tm3Grid/Notch2tm3Grid (more )
Note: Conditional mutant. Double labeled: red - Grhl2; green - Pcna (assay MGI:6156396).
Structure Level Pattern Image Note
TS27: adenohypophysis Present Regionally restricted 7L Expression in the cleft cells is the same as in wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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