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Gene Expression Data
Assay Details
Assay
Reference: J:159506 Sun P, et al., Cytokeratin expression during mouse embryonic and early postnatal mammary gland development. Histochem Cell Biol. 2010 Feb;133(2):213-21
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5827742
Gene symbol: Krt14
Gene name: keratin 14
Antibody: anti-keratin 14 (chicken)
Detection system: Secondary antibody coupled to rhodamine
Results
Specimen 1A: embryonic day 15.5 (more )
Note: Less developed mammary buds. Triple labeled: red - Krt14; blue - Krt6 (assay MGI:5827731); green - Krt8 (assay MGI:5827740).
Structure Level Pattern Image Note
TS23: mammary gland primordium Present 1A
Specimen 1B: embryonic day 15.5 (more )
Note: More advanced mammary buds. Triple labeled: red - Krt14; blue - Krt6 (assay MGI:5827731); green - Krt8 (assay MGI:5827740).
Structure Level Pattern Image Note
TS23: mammary gland primordium Present 1B
Specimen 1C: embryonic day 16.5 (more )
Note: Double labeled: red - Krt14; green - Krt6 (assay MGI:5827739).
Structure Level Pattern Image Note
TS24: mammary gland primordium Present 1C
Specimen 1D: embryonic day 18.5 (more )
Note: Triple labeled: red - Krt14; blue - Krt6 (assay MGI:5827731); green - Krt8 (assay MGI:5827740).
Structure Level Pattern Image Note
TS26: mammary gland Present 1D Mostly occupying the outer layers (Krt8-).
Specimen 1E: embryonic day 18.5 (more )
Note: Triple labeled: red - Krt14; blue - Krt6 (assay MGI:5827731); green - Krt8 (assay MGI:5827740).
Structure Level Pattern Image Note
TS26: mammary gland Present 1E Mostly occupying the outer layers (Krt8-). Many were also in the inner layer and some of them co-expressed Krt8.
Specimen 1F: embryonic day 18.5 (more )
Note: Double labeled: red - Krt14; green - Krt6 (assay MGI:5827739).
Structure Level Pattern Image Note
TS26: mammary gland Present 1F Mostly occupying the outer layers (Krt6-). Many were also in the inner layer.
Specimen 1G: embryonic day 18.5 (more )
Note: Double labeled: red - Krt14; green - Krt6 (assay MGI:5827739).
Structure Level Pattern Image Note
TS26: mammary gland Present 1G Mostly occupying the outer layers (Krt6-). Many were also in the inner layer.
Specimen 1H: embryonic day 18.5 (more )
Note: Double labeled: red - Krt14; green - Krt8 (assay MGI:5827740).
Structure Level Pattern Image Note
TS26: mammary gland Present 1H Mostly occupying the outer layers (Krt8-). Many were also in the inner layer and some of them co-expressed Krt8.
Specimen 1I: embryonic day 18.5 (more )
Note: Double labeled: red - Krt14; green - Krt19 (assay MGI:5827746).
Structure Level Pattern Image Note
TS26: mammary gland Present 1I Three populations were seen, including Krt14+Krt19+, Krt14+Krt19-, Krt14-Krt19+.
Specimen 1J: postnatal day 0 (more )
Note: Triple labeled: red - Krt14; blue - Krt6 (assay MGI:5827731); green - Krt8 (assay MGI:5827740).
Structure Level Pattern Image Note
TS27: mammary gland Present 1J Mostly occupying the outer layers (Krt8-). Many were also in the inner layer and some of them co-expressed Krt8.
Specimen 2C: postnatal day 14 (more )
Note: Double labeled: red - Krt14; green - Krt6 (assay MGI:5827739).
Structure Level Pattern Image Note
TS28: mammary gland Present 2C
Specimen 2D: postnatal day 18 (more )
Note: Double labeled: red - Krt14; green - Krt6 (assay MGI:5827739).
Structure Level Pattern Image Note
TS28: mammary gland Present 2D
Specimen 2E: postnatal week 3 (more )
Note: Double labeled: red - Krt14; green - Krt6 (assay MGI:5827739).
Structure Level Pattern Image Note
TS28: mammary gland duct Present 2E
Specimen 2F: postnatal week 12 (more )
Note: Double labeled: red - Krt14; green - Krt6 (assay MGI:5827739).
Structure Level Pattern Image Note
TS28: mammary gland Present 2F
Specimen 3A: embryonic day 15.5 (more )
Note: Double labeled: red - Krt14; green - Krt5 (assay MGI:5827757).
Structure Level Pattern Image Note
TS23: mammary gland primordium epithelium Present Widespread 3A Nearly all epithelial cells expressed both Krt5 and Krt14, with some cells displaying stronger Krt14 while others stronger Krt5 signals.
Specimen 3B: embryonic day 18.5 (more )
Note: Double labeled: red - Krt14; green - Krt5 (assay MGI:5827757).
Structure Level Pattern Image Note
TS26: mammary gland epithelium Present Widespread 3B Nearly all epithelial cells expressed both Krt5 and Krt14, with some cells displaying stronger Krt14 while others stronger Krt5 signals.
Specimen 3C: postnatal day 11 (more )
Note: Double labeled: red - Krt14; green - Krt5 (assay MGI:5827757).
Structure Level Pattern Image Note
TS28: mammary gland epithelium Present Widespread 3C Nearly all epithelial cells expressed both Krt5 and Krt14, with some cells displaying stronger Krt14 while others stronger Krt5 signals.
Specimen 3D: postnatal day 14 (more )
Note: Double labeled: red - Krt14; green - Krt5 (assay MGI:5827757).
Structure Level Pattern Image Note
TS28: mammary gland epithelium Present Widespread 3D The outer basal/myoepithelial cells expressed both Krt5 and Krt14, whereas some suprabasal/luminal cells expressed Krt14 but not Krt5.
Specimen 3E: postnatal day 18 (more )
Note: Double labeled: red - Krt14; green - Krt5 (assay MGI:5827757).
Structure Level Pattern Image Note
TS28: mammary gland epithelium Present Widespread 3E The outer basal/myoepithelial cells expressed both Krt5 and Krt14, whereas some suprabasal/luminal cells expressed Krt14 but not Krt5.
Specimen 3F: postnatal week 3 (more )
Note: Double labeled: red - Krt14; green - Krt5 (assay MGI:5827757).
Structure Level Pattern Image Note
TS28: mammary gland epithelium Present Widespread 3F The outer basal/myoepithelial cells expressed both Krt5 and Krt14, whereas some suprabasal/luminal cells expressed Krt14 but not Krt5.
Specimen 3G: postnatal week 8 (more )
Note: Double labeled: red - Krt14; green - Krt5 (assay MGI:5827757).
Structure Level Pattern Image Note
TS28: mammary gland epithelium Present Regionally restricted 3G Expressed in the basal compartment. The number of suprabasal/luminal Krt14+ cells dramatically decreased, few such cells were found.
Specimen not shown: postnatal day 11 (more )
Structure Level Pattern Image Note
TS28: mammary gland Present
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
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