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Gene Expression Data
Assay Details
Assay
Reference: J:159506 Sun P, et al., Cytokeratin expression during mouse embryonic and early postnatal mammary gland development. Histochem Cell Biol. 2010 Feb;133(2):213-21
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5827731
Gene symbol: Krt6
Gene name: keratin 6
Antibody: anti-K6
Detection system: Secondary antibody coupled to other - see notes
Assay notes: AMCA-conjugated goat anti-rabbit secondary antibody was used.
Results
Specimen 1A: embryonic day 15.5 (more )
Note: Less developed mammary buds. Triple labeled: blue - Krt6; red - Krt14 (assay MGI:5827742); green - Krt8 (assay MGI:5827740).
Structure Level Pattern Image Note
TS23: epidermis Present Regionally restricted 1A Expressed in skin periderm.
TS23: mammary gland primordium Ambiguous 1A Rarely detectable.
Specimen 1B: embryonic day 15.5 (more )
Note: More advanced mammary buds. Triple labeled: blue - Krt6; red - Krt14 (assay MGI:5827742); green - Krt8 (assay MGI:5827740).
Structure Level Pattern Image Note
TS23: epidermis Present Regionally restricted 1B Expressed in skin periderm.
TS23: mammary gland primordium Ambiguous 1B Rarely detectable.
Specimen 1D: embryonic day 18.5 (more )
Note: Triple labeled: blue - Krt6; red - Krt14 (assay MGI:5827742); green - Krt8 (assay MGI:5827740).
Structure Level Pattern Image Note
TS26: mammary gland Present 1D More abundant expression.
Specimen 1E: embryonic day 18.5 (more )
Note: Triple labeled: blue - Krt6; red - Krt14 (assay MGI:5827742); green - Krt8 (assay MGI:5827740).
Structure Level Pattern Image Note
TS26: mammary gland Present Regionally restricted 1E More abundant expression. Distribution showed regional variation but an enrichment in the inner layers.
Specimen 1J: postnatal day 0 (more )
Note: Triple labeled: blue - Krt6; red - Krt14 (assay MGI:5827742); green - Krt8 (assay MGI:5827740).
Structure Level Pattern Image Note
TS27: mammary gland Present Regionally restricted 1J More abundant expression. Distribution showed regional variation but an enrichment in the inner layers, largely distinct from the Krt14+ cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
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