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Gene Expression Data
Assay Details
Assay
Reference: J:159506 Sun P, et al., Cytokeratin expression during mouse embryonic and early postnatal mammary gland development. Histochem Cell Biol. 2010 Feb;133(2):213-21
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5827740
Gene symbol: Krt8
Gene name: keratin 8
Antibody: TROMA 1
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 1A: embryonic day 15.5 (more )
Note: Less developed mammary buds. Triple labeled: green - Krt8; red - Krt14 (assay MGI:5827742); blue - Krt6 (assay MGI:5827731).
Structure Level Pattern Image Note
TS23: mammary bud Absent 1A Absent in less developed mammary buds.
Specimen 1B: embryonic day 15.5 (more )
Note: More advanced mammary buds. Triple labeled: green - Krt8; red - Krt14 (assay MGI:5827742); blue - Krt6 (assay MGI:5827731).
Structure Level Pattern Image Note
TS23: mammary gland primordium Present 1B Present in more advanced mammary buds. Coexpressed in most Krt14+ cells.
Specimen 1D: embryonic day 18.5 (more )
Note: Triple labeled: green - Krt8; red - Krt14 (assay MGI:5827742); blue - Krt6 (assay MGI:5827731).
Structure Level Pattern Image Note
TS26: mammary gland Present Regionally restricted 1D Expressed in a luminal-like location, physically separated from the outer layer Krt14+ cells.
Specimen 1E: embryonic day 18.5 (more )
Note: Triple labeled: green - Krt8; red - Krt14 (assay MGI:5827742); blue - Krt6 (assay MGI:5827731).
Structure Level Pattern Image Note
TS26: mammary gland Present Regionally restricted 1E Expressed in a luminal-like location, physically separated from the outer layer Krt14+ cells.
Specimen 1H: embryonic day 18.5 (more )
Note: Double labeled: green - Krt8; red - Krt14 (assay MGI:5827742).
Structure Level Pattern Image Note
TS26: mammary gland Present Regionally restricted 1H Expressed in a luminal-like location, physically separated from the outer layer Krt14+ cells.
Specimen 1J: postnatal day 0 (more )
Note: Triple labeled: green - Krt8; red - Krt14 (assay MGI:5827742); blue - Krt6 (assay MGI:5827731).
Structure Level Pattern Image Note
TS27: mammary gland Present Regionally restricted 1I Expressed in a luminal-like location, physically separated from the outer layer Krt14+ cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
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