Gene Expression Data
Assay Details
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Assay
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Reference:
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J:159506
Sun P, et al., Cytokeratin expression during mouse embryonic and early postnatal mammary gland development. Histochem Cell Biol. 2010 Feb;133(2):213-21
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5827757
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Gene symbol:
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Krt5
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Gene name:
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keratin 5
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Antibody:
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Anti-Krt5
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
3A:
embryonic day 15.5
(more  )
Note: Double labeled: green - Krt5; red - Krt14 (assay MGI:5827742).
Specimen
3A:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 15.5
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Krt5; red - Krt14 (assay MGI:5827742).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: mammary gland primordium epithelium |
Present |
Widespread |
3A
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Nearly all epithelial cells expressed both Krt5 and Krt14, with some cells displaying stronger Krt14 while others stronger Krt5 signals.
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Specimen
3B:
embryonic day 18.5
(more )
Note: Double labeled: green - Krt5; red - Krt14 (assay MGI:5827742).
Specimen
3B:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Krt5; red - Krt14 (assay MGI:5827742).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: mammary gland epithelium |
Present |
Widespread |
3B
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Nearly all epithelial cells expressed both Krt5 and Krt14, with some cells displaying stronger Krt14 while others stronger Krt5 signals.
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Specimen
3C:
postnatal day 11
(more )
Note: Double labeled: green - Krt5; red - Krt14 (assay MGI:5827742).
Specimen
3C:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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postnatal day 11
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Krt5; red - Krt14 (assay MGI:5827742).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: mammary gland epithelium |
Present |
Widespread |
3C
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Nearly all epithelial cells expressed both Krt5 and Krt14, with some cells displaying stronger Krt14 while others stronger Krt5 signals.
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Specimen
3D:
postnatal day 14
(more )
Note: Double labeled: green - Krt5; red - Krt14 (assay MGI:5827742).
Specimen
3D:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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postnatal day 14
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Krt5; red - Krt14 (assay MGI:5827742).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: mammary gland epithelium |
Present |
Regionally restricted |
3D
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The outer basal/myoepithelial cells expressed both Krt5 and Krt14, whereas some suprabasal/luminal cells expressed Krt14 but not Krt5.
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Specimen
3E:
postnatal day 18
(more )
Note: Double labeled: green - Krt5; red - Krt14 (assay MGI:5827742).
Specimen
3E:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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postnatal day 18
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Krt5; red - Krt14 (assay MGI:5827742).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: mammary gland epithelium |
Present |
Regionally restricted |
3E
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The outer basal/myoepithelial cells expressed both Krt5 and Krt14, whereas some suprabasal/luminal cells expressed Krt14 but not Krt5.
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Specimen
3F:
postnatal week 3
(more )
Note: Double labeled: green - Krt5; red - Krt14 (assay MGI:5827742).
Specimen
3F:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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postnatal week 3
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Krt5; red - Krt14 (assay MGI:5827742).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: mammary gland epithelium |
Present |
Regionally restricted |
3F
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The outer basal/myoepithelial cells expressed both Krt5 and Krt14, whereas some suprabasal/luminal cells expressed Krt14 but not Krt5.
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Specimen
3G:
postnatal week 8
(more )
Note: Double labeled: green - Krt5; red - Krt14 (assay MGI:5827742).
Specimen
3G:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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postnatal week 8
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Krt5; red - Krt14 (assay MGI:5827742).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: mammary gland epithelium |
Present |
Regionally restricted |
3G
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Expression was near-completely confined to the basal compartment.
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