Probe:	IMAGE clone 1764103
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS13: extraembryonic component	Strong	Regionally restricted	2D	There is a strong signal in the ectoplacental cone and chorion, the expression in amnion is weak.
TS13: yolk sac	Weak		2D

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present		2E

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	2G	Expression is in the diencephalon.
TS13: future midbrain	Present		2G
TS13: head mesenchyme	Weak		2G

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present	Graded	3B	Expression is stronger in the dorsal and posterior side.
TS15: diencephalon	Present	Graded	3B	Expression is stronger in the ventral thalamus.
TS15: otocyst	Strong	Regionally restricted	3B	Expression is in the medial and dorsal part.
TS15: forelimb bud	Present		3B
TS15: hindlimb ridge	Present		3B
TS15: blood vessel	Weak	Regionally restricted	3B	Expression is in the vascular plexus surrounding brain neuroepithelium.

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present	Graded	3D	Expression is stronger in the dorsal and posterior side.
TS17: diencephalon	Present	Graded	3D	Expression is stronger in the ventral thalamus.
TS17: otocyst	Strong	Regionally restricted	3D	Expression is in the medial and dorsal part.
TS17: 1st branchial arch	Present		3D
TS17: 2nd branchial arch	Present		3D
TS17: forelimb bud	Present		3D
TS17: hindlimb bud	Present		3D
TS17: tail	Present		3D
TS17: genitourinary system	Present	Regionally restricted	3D	Expression is in the genital tubercle.
TS17: blood vessel	Present	Regionally restricted	3D	Expression is in the vascular plexus surrounding brain neuroepithelium.

Structure	Level	Pattern	Image	Note
TS17: apical ectodermal ridge	Strong		3E
TS17: limb mesenchyme	Present		3E

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present		3G
TS19: forebrain	Present	Regionally restricted	3G	Expression is in the hippocampal primordium.
TS19: 1st branchial arch	Present	Regionally restricted	3G	Expression is in the mesenchyme adjacent to epithelium.
TS19: 2nd branchial arch	Present	Regionally restricted	3G	Expression is in the mesenchyme adjacent to epithelium.
TS19: 3rd branchial arch	Present	Regionally restricted	3G	Expression is in the mesenchyme adjacent to epithelium.
TS19: ear	Present		3G
TS19: hindlimb bud	Present		3G
TS19: midbrain	Present		3G
TS19: diencephalon	Present		3G
TS19: blood vessel	Present	Regionally restricted	3G	Expression is in the vascular plexus surrounding the neuroepithelium.

Structure	Level	Pattern	Image	Note
TS19: 1st branchial arch	Present	Regionally restricted	3H	Expression is in the mesenchyme adjacent to epithelium.
TS19: 2nd branchial arch	Present	Regionally restricted	3H	Expression is in the mesenchyme adjacent to epithelium.
TS19: 3rd branchial arch	Present	Regionally restricted	3H	Expression is in the mesenchyme adjacent to epithelium.
TS19: endolymphatic appendage	Present		3H
TS19: blood vessel	Present	Regionally restricted	3H	Expression is in the vascular plexus surrounding the neuroepithelium.

Structure	Level	Pattern	Image	Note
TS21: forebrain	Present	Regionally restricted	4A	Expression is in the hippocampus.
TS21: midbrain	Present	Regionally restricted	4A	Expression is in the anterior tegmental neuroepithelium.
TS21: medulla oblongata	Present		4A
TS21: pons	Present		4A
TS21: spinal cord ventricular layer	Strong		4A
TS21: cardiovascular system	Strong	Regionally restricted	4A	Expression is in the vascular plexus and the capillaries invading the central nervous system, it is absent in other capillaries and the endothelium of large vessels.
TS21: diaphragm	Present		4A
TS21: vertebral cartilage condensation	Present		4A
TS21: genital tubercle	Strong	Regionally restricted	4A	Expression is in mesenchyme adjacent to surface epithelium.
TS21: lower jaw mesenchyme	Strong	Regionally restricted	4A	Expression is in mesenchyme adjacent to surface epithelium.
TS21: tail mesenchyme	Strong	Regionally restricted	4A	Expression is in mesenchyme adjacent to surface epithelium.
TS21: tongue mesenchyme	Strong	Regionally restricted	4A	Expression is in mesenchyme adjacent to surface epithelium.
TS21: upper jaw mesenchyme	Strong	Regionally restricted	4A	Expression is in mesenchyme adjacent to surface epithelium.

Structure	Level	Pattern	Image	Note
TS21: outflow tract	Strong	Regionally restricted	4B	Expression is in the valves of the outflow tract.
TS21: endocardial tissue	Absent		4B

Structure	Level	Pattern	Image	Note
TS21: lower jaw mesenchyme	Present	Regionally restricted	4C	Expression is in mesenchyme adjacent to surface epithelium.

Structure	Level	Pattern	Image	Note
TS21: spinal cord ventricular layer	Present		4D
TS21: cardiovascular system	Strong	Regionally restricted	4D	Expression is in the capillaries invading the central nervous system.

Structure	Level	Pattern	Image	Note
TS21: male reproductive system	Present	Regionally restricted	4E	Expression is in the mesenchyme surrounding Wolffian ducts.
TS21: mesenchyme of metanephros	Present	Regionally restricted	4E	Expression is in the mesenchyme surrounding the metanephric tubules.

Structure	Level	Pattern	Image	Note
TS21: mesenchyme of metanephros	Present	Regionally restricted	4F	Expression is in the mesenchyme surrounding the metanephric tubules.

Structure	Level	Pattern	Image	Note
TS21: male reproductive system	Present	Regionally restricted	4G	Expression is in the mesenchyme surrounding Wolffian ducts.
TS21: mesenchyme of metanephros	Present	Regionally restricted	4G	Expression is in the mesenchyme surrounding the metanephric tubules.

Structure	Level	Pattern	Image	Note
TS21: lung mesenchyme	Present	Regionally restricted	4H	Expression is in the mesnechyme surrounding the bronchi.

Structure	Level	Pattern	Image	Note
TS21: molar	Present	Regionally restricted	4I	Expression is in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: cap stage tooth	Present		4J
TS22: enamel knot	Present		4J
TS22: tooth mesenchyme	Present	Regionally restricted	4J	Expression is in the mesenchyme adjacent to the enamel knot.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present	Regionally restricted	5B	Expression is in the medial and dorsal part.

Structure	Level	Pattern	Image	Note
TS17: otocyst	Present	Regionally restricted	5C	Expression is in the medial and dorsal part.

Structure	Level	Pattern	Image	Note
TS19: endolymphatic appendage	Present		5D

Structure	Level	Pattern	Image	Note
TS21: inner ear	Present	Regionally restricted	5E	Expression is in the fusion plate region, between forming semicircular canals.

Structure	Level	Pattern	Image	Note
TS28: colon	Weak		6A
TS28: duodenum	Weak		6A
TS28: ileum	Weak		6A
TS28: jejunum	Weak		6A
TS28: rectum	Weak		6A

Structure	Level	Pattern	Image	Note
TS28: hair follicle	Present	Regionally restricted	6B	Expression is in the dermal papillae and matrix cells.