Spag16 MGI Mouse Gene Detail - MGI:1913972 - sperm associated antigen 16

Symbol

Spag16
Name

sperm associated antigen 16
Synonyms

4921511D23Rik, 4930524F24Rik, 4930585K05Rik, Pf20, Wdr29

Feature Type

protein coding gene
IDs

MGI:1913972
NCBI Gene: 66722
Alliance

gene page
Transcription Start Sites

10 TSS

Sequence Map

Chr1:69866129-70764291 bp, + strand
From Ensembl annotation of GRCm39
View this region in JBrowse
Genome Browsers

Ensembl | UCSC | NCBI

Genetic Map

Chromosome 1, 35.04 cM, cytoband C3
Mapping Data

4 experiments

SNPs within 2kb

5757 from dbSNP Build 142
Strain Annotations

18

For selected strains:

Strain	Gene Model ID	Feature Type	Coordinates
C57BL/6J	MGI_C57BL6J_1913972	protein coding gene	Chr1:69866031-70764291 (+)
129S1/SvImJ	MGP_129S1SvImJ_G0016122	protein coding gene	Chr1:70208647-71128235 (+)
A/J	MGP_AJ_G0016105	protein coding gene	Chr1:67719210-68610546 (+)
AKR/J	MGP_AKRJ_G0016062	protein coding gene	Chr1:69666062-70596369 (+)
BALB/cJ	MGP_BALBcJ_G0016060	protein coding gene	Chr1:67823715-68729475 (+)
C3H/HeJ	MGP_C3HHeJ_G0015891	protein coding gene	Chr1:69990563-70918988 (+)
C57BL/6NJ	MGP_C57BL6NJ_G0016513	protein coding gene	Chr1:72886704-73858830 (+)
CAROLI/EiJ	MGP_CAROLIEiJ_G0014234	protein coding gene	Chr1:63472678-64344631 (+)
CAST/EiJ	MGP_CASTEiJ_G0015475	protein coding gene	Chr1:69212220-70159136 (+)
CBA/J	MGP_CBAJ_G0015864	protein coding gene	Chr1:75329852-76327005 (+)
DBA/2J	MGP_DBA2J_G0015965	protein coding gene	Chr1:67109780-68016445 (+)
FVB/NJ	MGP_FVBNJ_G0015968	protein coding gene	Chr1:66301066-67195680 (+)
LP/J	MGP_LPJ_G0016037	protein coding gene	Chr1:71260446-72186381 (+)
NOD/ShiLtJ	MGP_NODShiLtJ_G0015988	protein coding gene	Chr1:78709370-79837894 (+)
NZO/HlLtJ	MGP_NZOHlLtJ_G0016560	protein coding gene	Chr1:69638256-70570795 (+)
PWK/PhJ	MGP_PWKPhJ_G0015262	protein coding gene	Chr1:66512117-67418564 (+)
SPRET/EiJ	MGP_SPRETEiJ_G0015041	protein coding gene	Chr1:69027784-69980517 (+)
WSB/EiJ	MGP_WSBEiJ_G0015537	protein coding gene	Chr1:69442709-70383709 (+)

Human Ortholog

SPAG16, sperm associated antigen 16

Vertebrate Orthologs

2

Vertebrate Orthology Source

Alliance of Genome Resources

Human Ortholog

SPAG16, sperm associated antigen 16
Synonyms

PF20, WDR29
Links

HGNC:23225

NCBI Gene ID: 79582

neXtProt AC: NX_Q8N0X2

UniProt: Q8N0X2
Chr Location

2q34; chr2:213284389-214410501 (+) GRCh38

MGI Vertebrate Homology

Spag16 stringent orthology
1 human;1 mouse;1 rat
HCOP

vertebrate homology predictions: SPAG16
Gene Tree

Spag16

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Phenotype Summary

16 phenotypes from 2 alleles in 2 genetic backgrounds
18 phenotype references

Phenotype Overview

Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene.

adipose tissue	behavior/neurological	cardiovascular system	cellular	craniofacial	digestive/alimentary system	embryo	endocrine/exocrine glands	growth/size/body	hearing/vestibular/ear	hematopoietic system	homeostasis/metabolism	integument	immune system	limbs/digits/tail	liver/biliary system	mortality/aging	muscle	nervous system	pigmentation	renal/urinary system	reproductive system	respiratory system	skeleton	taste/olfaction	neoplasm	vision/eye

Click cells to view annotations.

All Mutations and Alleles

9
Chemically induced (other)

1
Endonuclease-mediated

2
Gene trapped

1
Radiation induced

1
Targeted

4
Genomic Mutations

2 involving Spag16
Incidental Mutations

Mutagenetix , APF , CvDC
Find Mice (IMSR)

66 strains or lines available
Comparison Matrix

Gene Expression + Phenotype

Chimeric males carrying one copy of the mutated allele have impaired spermatogenesis, a significant loss of germ cells at the round spermatid stage, and disorganized sperm axoneme structure. No offspring carrying the mutated allele are produced from matings using male chimeras.

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All GO Annotations

22
GO References

12

Molecular Function

carbohydrate derivative binding	cytoskeletal protein binding	DNA binding	enzyme regulator	hydrolase	ligase	lipid binding	oxidoreductase	RNA binding	signaling receptor activity	signaling receptor binding	transcription	transferase	transporter

Biological Process

carbohydrate derivative metabolism	cell differentiation	cell population proliferation	cellular component organization	DNA-templated transcription	establishment of localization	homeostatic process	immune system process	lipid metabolic process	programmed cell death	protein metabolic process	response to stimulus	signaling	system development

Cellular Component

cell projection	cytoplasmic vesicle	cytoskeleton	cytosol	endoplasmic reticulum	endosome	extracellular region	Golgi apparatus	mitochondrion	non-membrane-bounded organelle	nucleus	organelle envelope	organelle lumen	plasma membrane	protein-containing complex	synapse	vacuole

Click cells to view annotations.

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Expression Overview

GXD's primary emphasis is on endogenous gene expression during development. Click on grid cells to view annotations.

Blue cells = expressed in wild-type.
Gray triangles = other expression annotations only
(e.g. absence of expression or data from mutants).

early conceptus	embryo ectoderm	embryo endoderm	embryo mesoderm	embryo mesenchyme	extraembryonic component	alimentary system	auditory system	branchial arches	cardiovascular system	connective tissue	endocrine system	exocrine system	hemolymphoid system	integumental system	limbs	liver and biliary system	musculoskeletal system	nervous system	olfactory system	reproductive system	respiratory system	urinary system	visual system

Click cells to view annotations.

Assay Results

706
cDNA Data

17
Literature Summary

1

Tissue x Stage Matrix

Comparison Matrix

Gene Expression + Phenotype

Other Mouse Links

Allen Institute

GEO

Expression Atlas
Other Vertebrate Links

Xenbase spag16

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All Sequences

53
RefSeq

8
UniProt

3
CCDS

CCDS15027.1, CCDS69901.1

Ensembl

ENSMUSG00000053153 (Evidence)
NCBI Gene

66722

			Length	Strain/Species	Flank
genomic	ENSMUSG00000053153	Ensembl Gene Model \| MGI Sequence Detail	898163	C57BL/6J	± kb
transcript	ENSMUST00000065425	Ensembl \| MGI Sequence Detail	2126	Not Applicable
polypeptide	ENSMUSP00000069821	Ensembl \| MGI Sequence Detail	639	Not Applicable

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UniProt

3 Sequences
Protein Ontology

PR:000015455 sperm-associated antigen 16 protein

(term hierarchy)
InterPro Domains

IPR020472 G-protein beta WD-40 repeat

IPR001680 WD40 repeat

IPR019775 WD40 repeat, conserved site

IPR036322 WD40-repeat-containing domain superfamily

IPR015943 WD40/YVTN repeat-like-containing domain superfamily

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All nucleic 18

cDNA 17

Primer pair 1

Microarray probesets 5

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MGI:1922324, MGI:1923198, MGI:2138527

Summaries

All 44

Developmental Gene Expression 1

Gene Ontology 12

Phenotypes 18
Earliest

J:134667 Roderick TH, et al., Two radiation-induced chromosomal inversions in mice (Mus musculus). Proc Natl Acad Sci U S A. 1970 Oct;67(2):961-7
Latest

J:307547 Miyata H, et al., Analysis of the sperm flagellar axoneme using gene-modified mice. Exp Anim. 2020 Nov 12;69(4):374-381

TSS for Spag16:

(View these features in JBrowse)

Transcription Start Site	Location	Distance from Gene 5'-end
Tssr7261	Chr1:69866024-69866074 (+)	-80 bp
Tssr7262	Chr1:69866116-69866143 (+)	1 bp
Tssr7263	Chr1:69866145-69866162 (+)	25 bp
Tssr7264	Chr1:69956222-69956229 (+)	90,097 bp
Tssr7265	Chr1:69990770-69990794 (+)	124,653 bp
Tssr7266	Chr1:69990800-69990806 (+)	124,674 bp
Tssr7267	Chr1:70256461-70256464 (+)	390,334 bp
Tssr112	Chr1:70259906-70259910 (+)	393,779 bp
Tssr113	Chr1:70582352-70582354 (+)	716,224 bp
Tssr7268	Chr1:70608113-70608115 (+)	741,985 bp

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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