Map4k4 MGI Mouse Gene Detail - MGI:1349394 - mitogen-activated protein kinase kinase kinase kinase 4

Symbol

Map4k4
Name

mitogen-activated protein kinase kinase kinase kinase 4
Synonyms

9430080K19Rik, Nik

Feature Type

protein coding gene
IDs

MGI:1349394
NCBI Gene: 26921
Alliance

genome feature page
Transcription Start Sites

14 TSS

Sequence Map

Chr1:39940073-40065470 bp, + strand
From Ensembl annotation of GRCm39
View this region in JBrowse
Genome Browsers

Ensembl | UCSC | NCBI

Genetic Map

Chromosome 1, 18.53 cM, cytoband B
Mapping Data

3 experiments

SNPs within 2kb

3830 from dbSNP Build 142
Strain Annotations

19

For selected strains:

Strain	Gene Model ID	Feature Type	Coordinates
C57BL/6J	MGI_C57BL6J_1349394	protein coding gene	Chr1:39939806-40065470 (+)
129S1/SvImJ	ENSMUSG00200003232	protein coding gene	Chr1:37535550-37661160 (+)
A/J	ENSMUSG00195042489	protein coding gene	Chr1:37208979-37336549 (+)
AKR/J	ENSMUSG00220009007	protein coding gene	Chr1:37199377-37324726 (+)
BALB/cJ	ENSMUSG00180011241	protein coding gene	Chr1:37279046-37404513 (+)
C3H/HeJ	ENSMUSG00175001293	protein coding gene	Chr1:37212017-37339565 (+)
C57BL/6NJ	ENSMUSG00215019456	protein coding gene	Chr1:37254147-37379628 (+)
CAROLI/EiJ	MGP_CAROLIEiJ_G0014076	protein coding gene	Chr1:34532666-34657413 (+)
CAST/EiJ	ENSTCUG00005002990	protein coding gene	Chr1:37327176-37456572 (+)
CBA/J	ENSMUSG00210005587	protein coding gene	Chr1:37015956-37141292 (+)
DBA/2J	ENSMUSG00185009784	protein coding gene	Chr1:38118073-38243539 (+)
FVB/NJ	ENSMUSG00205021558	protein coding gene	Chr1:37223862-37349770 (+)
JF1/MsJ	ENSUMUG00000010831	protein coding gene	Chr1:37334183-37463456 (+)
LP/J	ENSMUSG00230024016	protein coding gene	Chr1:38153554-38278912 (+)
NOD/ShiLtJ	ENSMUSG00190024493	protein coding gene	Chr1:37232789-37358672 (+)
NZO/HlLtJ	ENSMUSG00225000393	protein coding gene	Chr1:41413192-41538807 (+)
PWK/PhJ	ENSLUMG00010030546	protein coding gene	Chr1:36860679-36989801 (+)
SPRET/EiJ	ENSMSPG00010027055	protein coding gene	Chr1:38333805-38461210 (+)
WSB/EiJ	ENSIUOG00005033253	protein coding gene	Chr1:37155353-37280555 (+)

Human Ortholog

MAP4K4, mitogen-activated protein kinase kinase kinase kinase 4

Vertebrate Orthologs

3

Vertebrate Orthology Source

Alliance of Genome Resources

Human Ortholog

MAP4K4, mitogen-activated protein kinase kinase kinase kinase 4
Synonyms

FLH21957, HEL-S-31, HGK, MEKKK4, NIK
Links

HGNC:6866

NCBI Gene ID: 9448

UniProt: O95819
Chr Location

2q11.2; chr2:101696850-101894690 (+) GRCh38

MGI Vertebrate Homology

Map4k4 stringent orthology
1 human;1 mouse;1 rat;1 zebrafish
HCOP

vertebrate homology predictions: MAP4K4
Gene Tree

Map4k4

Diseases

2 with human MAP4K4 associations

				Human Disease	Mouse Models
				hepatocellular carcinoma IDs hepatocellular carcinoma DOID:684 DOID:5005 EFO:0000182 ICD10CM:C22.0 MESH:D006528 NCI:C3099 OMIM:114550 ORDO:88673 UMLS_CUI:C2239176
				lung adenocarcinoma IDs lung adenocarcinoma DOID:3910 DOID:4826 EFO:0000571 MESH:D000077192 NCI:C27745 NCI:C3512 UMLS_CUI:C0152013 UMLS_CUI:C1335060

Click on a disease name to see all genes associated with that disease.

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Phenotype Summary

6 phenotypes from 1 allele in 1 genetic background
26 phenotype references

Phenotype Overview

Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene.

adipose tissue	behavior/neurological	cardiovascular system	cellular	craniofacial	digestive/alimentary system	embryo	endocrine/exocrine glands	growth/size/body	hearing/vestibular/ear	hematopoietic system	homeostasis/metabolism	integument	immune system	limbs/digits/tail	liver/biliary system	mortality/aging	muscle	nervous system	pigmentation	renal/urinary system	reproductive system	respiratory system	skeleton	taste/olfaction	neoplasm	vision/eye

Click cells to view annotations.

All Mutations and Alleles

53
Chemically induced (ENU)

1
Chemically induced (other)

1
Endonuclease-mediated

7
Gene trapped

38
Targeted

6
Genomic Mutations

1 involving Map4k4
Incidental Mutations

Mutagenetix , APF , CvDC
Find Mice (IMSR)

105 strains or lines available
Comparison Matrix

Gene Expression + Phenotype

Mice homozygous for disruptions in this gene die as embryos around day E9.5-10.5.

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All GO Annotations

42
GO References

12

Molecular Function

carbohydrate derivative binding	cytoskeletal protein binding	DNA binding	enzyme regulator	hydrolase	ligase	lipid binding	oxidoreductase	RNA binding	signaling receptor activity	signaling receptor binding	transcription	transferase	transporter

Biological Process

carbohydrate derivative metabolism	cell differentiation	cell population proliferation	cellular component organization	DNA-templated transcription	establishment of localization	homeostatic process	immune system process	lipid metabolic process	programmed cell death	protein metabolic process	response to stimulus	signaling	system development

Cellular Component

cell projection	cytoplasmic vesicle	cytoskeleton	cytosol	endoplasmic reticulum	endosome	extracellular region	Golgi apparatus	mitochondrion	membraneless organelle	nucleus	organelle envelope	organelle lumen	plasma membrane	protein-containing complex	synapse	vacuole

Click cells to view annotations.

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Expression Overview

GXD's primary emphasis is on endogenous gene expression during development. Click on grid cells to view annotations.

Blue cells = expressed in wild-type.
Gray triangles = other expression annotations only
(e.g. absence of expression or data from mutants).

early conceptus	embryo ectoderm	embryo endoderm	embryo mesoderm	embryo mesenchyme	extraembryonic component	alimentary system	auditory system	branchial arches	cardiovascular system	connective tissue	endocrine system	exocrine system	hemolymphoid system	integumental system	limbs	liver and biliary system	musculoskeletal system	nervous system	olfactory system	reproductive system	respiratory system	urinary system	visual system

Click cells to view annotations.

Assay Results

776
Tissues

44
cDNA Data

21
Literature Summary

7

Images

Tissue x Stage Matrix

Comparison Matrix

Gene Expression + Phenotype

Other Mouse Links

Allen Institute

GEO

Expression Atlas
Other Vertebrate Links

Xenbase map4k4

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All Sequences

199
RefSeq

100
UniProt

16
CCDS

CCDS35546.1, CCDS78573.1, CCDS78575.1, CCDS78576.1

Ensembl

ENSMUSG00000026074 (Evidence)
NCBI Gene

26921

			Length	Strain/Species	Flank
genomic	ENSMUSG00000026074	Ensembl Gene Model \| MGI Sequence Detail	125398	C57BL/6J	± kb
transcript	ENSMUST00000163854	Ensembl \| MGI Sequence Detail	5782	Not Applicable
polypeptide	ENSMUSP00000126961	Ensembl \| MGI Sequence Detail	1288	Not Applicable

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UniProt

16 Sequences
Protein Ontology

PR:000003043 mitogen-activated protein kinase kinase kinase kinase 4

(term hierarchy)
EC

2.7.11.1
InterPro Domains

IPR001180 Citron homology (CNH) domain

IPR017441 Protein kinase, ATP binding site

IPR000719 Protein kinase domain

IPR011009 Protein kinase-like domain superfamily

IPR008271 Serine/threonine-protein kinase, active site

IPR051700 STE20 Ser/Thr Protein Kinase
GlyGen

P97820 2 sites, 1 N-linked glycan (1 site), 1 O-linked glycan (1 site)

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All nucleic 27

cDNA 26

Primer pair 1

Microarray probesets 7

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MGD-MRK-39589, MGI:109590, MGI:1915370, MGI:2138498, MGI:2138507, MGI:2138541

Summaries

All 76

Developmental Gene Expression 7

Gene Ontology 12

Phenotypes 26
Earliest

J:39300 Su YC, et al., NIK is a new Ste20-related kinase that binds NCK and MEKK1 and activates the SAPK/JNK cascade via a conserved regulatory domain. EMBO J. 1997 Mar 17;16(6):1279-90
Latest

J:389864 Murata K, et al., A novel miniTurbo knock-in mouse reveals a protein interaction network of USP46 in the brain. Exp Anim. 2026 Jan 1;75(1):63-72

TSS for Map4k4:

(View these features in JBrowse)

Transcription Start Site	Location	Distance from Gene 5'-end
Tssr6395	Chr1:39940043-39940123 (+)	10 bp
Tssr6396	Chr1:39940189-39940234 (+)	139 bp
Tssr6397	Chr1:39940236-39940260 (+)	175 bp
Tssr6398	Chr1:39940899-39940902 (+)	828 bp
Tssr6399	Chr1:39942602-39942620 (+)	2,538 bp
Tssr6400	Chr1:39943168-39943195 (+)	3,109 bp
Tssr6401	Chr1:39943282-39943324 (+)	3,230 bp
Tssr6402	Chr1:39946304-39946322 (+)	6,240 bp
Tssr6403	Chr1:40010526-40010527 (+)	70,454 bp
Tssr6404	Chr1:40025784-40025793 (+)	85,716 bp
Tssr6405	Chr1:40048976-40048991 (+)	108,911 bp
Tssr6406	Chr1:40049059-40049099 (+)	109,006 bp
Tssr6407	Chr1:40049205-40049223 (+)	109,141 bp
Tssr6408	Chr1:40064524-40064548 (+)	124,463 bp

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 09/01/2026 MGI 6.24