Mapping Data

Experiment

• Experiment
  TEXT
• Chromosome
  9
• Reference
  J:30687 Miner LL, et al., Chromosomal mapping of the psychomotor stimulant effects of cocaine in BXD recombinant inbred mice. Psychopharmacology (Berl). 1995 Dec;122(3):209-14
• ID
  MGI:3054677

Genes

Gene	Assay Type
D9Mit12	PCR amplified length variant
Rbp1	reported elsewhere
Rbp2	reported elsewhere
D9Ncvs48	PCR amplified length variant
D9Ncvs42	PCR amplified length variant
Drd2	reported elsewhere
Chrna5	reported elsewhere
Chrna3	reported elsewhere
Htr1b	reported elsewhere
Opcml	reported elsewhere
Cck	reported elsewhere

Notes

• Experiment
  Linkage analysis was performed on 11 BXD (B=C57BL/6J; D= DBA/2J) recombinant inbred (RI) strains to identify QTLs associated with locomotor activity before and after cocaine treatment. A panel of 2,000 polymorphic markers was used for genotyping. Animalswere assessed for distance traveled in an experimental chamber for 30 minutes. A cocaine solution (10 mg/kg) was then injected intraperitoneally and the animals were assessed for another 30 minutes. Parental strain DBA/2J exhibits increased locomotor activity both before and after cocaine treatment compared to parental strain C57BL/6J. The most significant associations mapped to 26 cM on mouse Chromosome 5 and to 45 cM on mouse Chromosome 9.
  
  The chromosome 5 locus is associated with cocaine-induced locomotor activity and shows linkage to markers Mpmv7 (24 cM), Pmv5 (25 cM), and D5Nds1 (24 cM). This locus reaches the statistical significance level of P<0.001. Association to baseline activity is also observed with a statistical significance level of P<0.05. A potential candidate gene found in this region of chromosome 5 is Drd5 (23 cM).
  
  The chromosome 9 locus is associated with baseline and cocaine-induced locomotor activity at a statistical significance level of P<0.01. This locus shows linkage to markers D9Mit12 (55 cM), Rbp1 (52 cM), Rbp2 (57 cM), D9Ncvs48 (58 cM), and D9Ncvs42 (58 cM). Potential candidate genes mapping near this region are Drd2 (28 cM), Chrna5 (32 cM), Chrna3 (51 cM), Htr1b (46 cM), Cck (71 cM), and Opcml (10 cM). This locus overlaps with a cocaine-induced locomotor activity QTL identified by Tolliver et al, 1994 (J:21289) on mouse Chromosome 9 spanning 38 cM - 57 cM.