Gene Expression Image Detail
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Reference
Figure
ID
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J:386951
Guertin TM, et al., Onset of embryonic and placental defects coincide in 19 of 22 novel mid-gestation lethal murine knockout lines. Development. 2026 May 15;153(10):dev205276
Extended Data 16
MGI:8377020
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| Caption | (A) Summary of defects observed in E9.5 and E10.5 Alg3-/- placentas. (B) Histological images of adjacent sections from a representative E9.5 Alg3-/- placenta demonstrating multiple LZ defects relative to controls (n = 3). Dashed boxes indicate the LZ region shown at higher magnification in C. In KO placentas, while the Tfeb+ region is relatively comparable to controls, the MCT1+ SynTI region is drastically reduced. While shallow projections of allantoic mesenchyme outlined in Gcm1/MCT4+ SynTII cells are observed, a lack of elongation and secondary branching prevent expansion of the network to the extent observed in controls. Although MCT1 and MCT4 signals are found in close apposition, the extensive network of adjacent MCT1+ SynTI and MCT4+ SynTII cells observed in controls has not developed. (D) Images of the embryonic component of the conceptuses shown in B and C. (E) Histological images of adjacent sections from a representative E10.5 Alg3-/- placenta displaying an abnormal Tpbp+ SpT layer and multiple LZ defects relative to controls (n = 3). Dashed boxes indicate the LZ region shown at higher magnification in F. An expanded Tpbp+ SpT layer is observed in 2 KO placentas whereas a reduction is observed in the other. In addition, the Tfeb+ and MCT1+ SynTI region is reduced compared to controls. However, Tfeb signal intensity within this domain appears elevated, suggesting an increased density of Tfeb+ cells despite the overall reduction in layer thickness. The SynTII layer/ chorioallantoic branching is also severely impaired, demonstrated by the limited Gcm1 and MCT4 expression. While endothelial cells, fetal and maternal blood cells are detected among labyrinth trophoblasts, discrete blood spaces are noticeably decreased. Although MCT1 and MCT4 signals are found in close apposition, the extensive network of adjacent MCT1+ SynTI and MCT4+ SynTII cells observed in controls is absent. (G) Images of the embryonic component of the conceptuses shown in E and F. (H) Examination of additional KO placentas (n = 3) reveals a thicker Pl-1+ TGC layer but a diminished Tpbp+ SpT layer, in contrast to the previously examined KO placentas exhibiting an expanded SpT layer. Scale bars: 500 m. |
| Copyright | This image is from Guertin TM, Development 2026 May 15;153(10):dev205276, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. |
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Associated Assays |
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 09/15/2026 MGI 6.29 |
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