Gene Expression Image Detail
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Reference
Figure
ID
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J:386951
Guertin TM, et al., Onset of embryonic and placental defects coincide in 19 of 22 novel mid-gestation lethal murine knockout lines. Development. 2026 May 15;153(10):dev205276
Extended Data 8
MGI:8377011
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| Image |
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| Caption | A) Summary of defects observed in E9.5 Chp1-/- placentas. The patterned overlay indicates the phenotype is observed in at least half but not all KO placentas examined. (B) Histological images of adjacent sections from a representative E9.5 Chp1-/- placenta displaying a diminished Tpbp + SpT layer, impaired chorioallantoic attachment, and multiple LZ defects relative to controls (n = 4). Dashed boxes indicate the LZ region shown at higher magnification in C. Impaired chorioallantoic attachment is observed in most KO conceptuses examined (KO #2, n = 3/4). Although allantoic mesenchyme is present adjacent to the chorion, the attachment appears weak and is easily disrupted during dissection. In the conceptus with normal attachment (KO #1, n = 1/4), less allantoic mesenchyme is observed, and the resulting interface fails to expand as widely as in controls. In the KO placentas, the Tfeb+ and MCT1+ SynTI region fails to expand and remains a thin layer compared to controls. Gcm1 and MCT4 are restricted to the basal chorion and the KO placenta with proper chorioallantoic attachment exhibits only shallow invasion of allantoic mesenchyme. This demonstrates that while chorioallantoic branching and SynTII differentiation initiate in these KOs, further elongation and secondary branching of these Gcm1/MCT4 SynTII outlined projections fail. Endothelial cells and fetal blood cells remain confined to the shallow projections of allantoic mesenchyme. Maternal blood similarly fails to integrate among labyrinth trophoblasts and instead accumulates between the JZ and LZ. Although the minimal MCT1 and MCT4 signals are found in close apposition, the extensive network of adjacent MCT1+ SynTI and MCT4+ SynTII cells observed in controls has not developed. (D) Images of the embryonic component of the conceptuses shown in B and C. (E) Examination of additional KO placentas demonstrates that a thinner Pl-1+ TGC layer accompanies the diminished Tpbp + SpT layer (n = 4). Scale bars: 500 m. |
| Copyright | This image is from Guertin TM, Development 2026 May 15;153(10):dev205276, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. |
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Associated Assays |
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 09/15/2026 MGI 6.29 |
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