Gene Expression Image Detail
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Reference
Figure
ID
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J:122205
Little MH, et al., Gene Expr Patterns. 2007 Jun;7(6):680-99
7
MGI:4849267
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| Caption | (A) Wnt7b, Spint2, Lamb1-1 and Wnt4 are ventral views of the gonad-mesonephros. The dorsal view of Shh is shown to visualise the mesonephric tubules. Inset images in Wnt7b, Spint2 and Lamb1-1 are dorsal views of the rostral end of the samples to visualise the mesonephric tubules. Mesonephros: Black double arrowheads indicate expression seen in the nephric duct, mesonephric portion in both sexes, for Wnt7b, Spint2, Lamb1-1 and Shh. Black single arrowheads indicate expression seen in the paramesonephric duct in both sexes for Wnt7b, Spint2 and Lamb1-1. White arrowheads indicate expression in the mesonephric tubules for Wnt7b, Spint2, Lamb1-1 and Shh in both sexes. Expression in the mesonephric mesenchyme was seen for Lamb1-1 and Wnt4. The white outlined arrowheads in Wnt4 M and F indicate the strongest Wnt4 signal seen in the mesonephric mesenchyme, at the junction where it contacts the gonad in both sexes and in two lines of mesonephros running medial to and parallel with both the nephric duct and paramesonephric duct. Sectioning of the hybridised whole mount tissue confirmed that the Wnt4 expression was restricted to the mesonephric mesenchyme immediately surrounding the nephric duct and not in the duct epithelium. Gonad: In the male gonads, Spint2 was expressed in the primary sex cords of the testis and Lamb1-1 in the interstitium of the testis. In the female gonads, Spint2, Lamb1-1 and Wnt4 showed expression in the ovary. Scale bar = 25 microns. (B) At TS20, Col6a1 was detected in the mesonephric mesenchyme (arrowheads), nephric duct and paramesonephric duct of both sexes, the interstitium of the testis (double arrowhead) and the mesenchymal stroma of the ovary. At TS23, expression persisted in the mesonephric mesenchyme of both sexes (arrowheads), the interstitium of the testis (double arrowhead) and both the cortical and medullary components of the mesenchymal stroma of the ovary. In the adult female ovary, Col6a1 expression was seen in the corpora lutea (arrowhead) and theca cells of pre-antral and antral follicles (double arrowhead). No expression was seen in the adult male testis (t) or epididymis (e). Kpna2 was detected in a spotted pattern in all embryonic tissue types, with strongest signal detected in the primary sex cord of the testis at TS20 (arrowhead), which was maintained in the seminiferous cords of the TS23 and adult testes (arrowhead). No Kpna2 expression was detected in the adult epididymis (e). The ovary showed Kpna2 expression at all. In the adult ovary, strongest Kpna2 signal was detected in the granulosa cells of all follicle states and weaker signal in the oocytes of follicles (arrowhead). Myh11, a marker of smooth muscle cells, was strongly expressed in the developing aorta and common iliac arteries adjacent to the TS20 male and female reproductive tracts. Expression was also detected in the developing vasculature of the testis and ovary at TS20 and TS23 (arrowheads). Expression was seen in the coelomic vessel of the testis at TS20 (arrowhead). In the adult, Myh11 expression was detected in the vasculature of the testis, epididymis, vas deferens in the male and ovary and oviduct in the female. Adult expression was also seen in the peritubular myoid cells of the seminiferous cords, the muscle layer of the epididymis (e, arrowhead) and the muscle layer of the ductus deferens (not shown) in the male and in the theca cells surrounding the follicles of the ovary (arrowhead) and in the circular and longitudinal muscle of the oviduct (not shown) in the female. Scale bar TS20 = 25 microns, TS23 = 200 microns, adult = 500 microns. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Copyright | Reprinted with permission from Elsevier from doi:10.1016/j.modgep.2007.03.002 Gene Expr Patterns 7: 680-99, Little MH; Brennan J; Georgas K; Davies JA; Davidson DR; Baldock RA; Beverdam A; Bertram JF; Capel B; Chiu HS; Clements D; Cullen-McEwen L; Fleming J; Gilbert T; Herzlinger D; Houghton D; Kaufman MH; Kleymenova E; Koopman PA; Lewis AG; McMahon AP; Mendelsohn CL; Mitchell EK; Rumballe BA; Sweeney DE; Valerius MT; Yamada G; Yang Y; Yu J, A high-resolution anatomical ontology of the developing murine genitourinary tract. Copyright 2007 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Associated Assays |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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