Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:122205
Little MH, et al., A high-resolution anatomical ontology of the developing murine genitourinary tract. Gene Expr Patterns. 2007 Jun;7(6):680-99
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:4849478
|
Gene symbol:
|
Wnt4
|
Gene name:
|
wingless-type MMTV integration site family, member 4
|
Probe:
|
GUDMAP Wnt4 probe
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
|
Results
|
Specimen
2B:
embryonic day 10.5
(more )
Specimen
2B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2C WM:
embryonic day 12.5
(more )
Specimen
2C WM:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2C SISH:
embryonic day 15.5
(more )
Specimen
2C SISH:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: cortical collecting duct |
Present |
|
2C Wnt4 TS23 upper
,
2C Wnt4 TS23 lower
|
|
TS23: medullary collecting duct |
Present |
|
2C Wnt4 TS23 upper
,
2C Wnt4 TS23 lower
|
|
TS23: renal cortical interstitium |
Present |
|
2C Wnt4 TS23 upper
,
2C Wnt4 TS23 lower
|
Expression is present in the cortical and medullary interstitium immediately surrounding the collecting ducts.
|
TS23: renal medullary interstitium |
Present |
|
2C Wnt4 TS23 upper
,
2C Wnt4 TS23 lower
|
Expression is present in the cortical and medullary interstitium immediately surrounding the collecting ducts.
|
TS23: pelvic urothelial lining |
Present |
|
2C Wnt4 TS23 upper
|
|
TS23: comma-shaped body |
Present |
|
2C Wnt4 TS23 lower
,
2C Wnt4 TS23 upper
|
Expression is present in early nephrons with expression in pretubular aggregates, renal vesicles, comma-shaped bodies and S-shaped bodies.
|
TS23: pretubular aggregate |
Present |
|
2C Wnt4 TS23 lower
,
2C Wnt4 TS23 upper
|
Expression is present in early nephrons with expression in pretubular aggregates, renal vesicles, comma-shaped bodies and S-shaped bodies.
|
TS23: renal vesicle |
Present |
|
2C Wnt4 TS23 lower
,
2C Wnt4 TS23 upper
|
Expression is present in early nephrons with expression in pretubular aggregates, renal vesicles, comma-shaped bodies and S-shaped bodies.
|
TS23: s-shaped body |
Present |
|
2C Wnt4 TS23 lower
,
2C Wnt4 TS23 upper
|
Expression is present in early nephrons with expression in pretubular aggregates, renal vesicles, comma-shaped bodies and S-shaped bodies.
|
Specimen
4D:
embryonic day 15.5
(more )
Specimen
4D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4L:
postnatal day 1
(more )
Specimen
4L:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: renal cortex |
Present |
Regionally restricted |
4L inset
,
4L
|
Expression is present in the early tubule.
|
Specimen
6B WM:
embryonic day 12.5
(more )
Specimen
6B WM:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
6B SISH:
embryonic day 15.5
(more )
Specimen
6B SISH:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
7A male:
embryonic day 12.5
(more )
Specimen
7A male:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Male
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: mesonephric mesenchyme of male |
Present |
Regionally restricted |
7A Wnt4 Male
|
Expression is restricted to the mesenchyme immediately surrounding the nephric duct and not in the duct epithelium. The strongest signal is seen at the junction where it contacts the gonad and in two lines running medial and parallel to the ducts.
|
Specimen
7A female:
embryonic day 12.5
(more )
Specimen
7A female:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Female
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: mesonephric mesenchyme of female |
Present |
Regionally restricted |
7A Wnt4 Female
|
Expression is restricted to the mesenchyme immediately surrounding the nephric duct and not in the duct epithelium. The strongest signal is seen at the junction where it contacts the gonad and in two lines running medial and parallel to the ducts.
|
TS20: ovary |
Present |
|
7A Wnt4 Female
|
|
|