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Gene Expression Data
Assay Details
Assay
Reference: J:253351 Hanley O, et al., Parallel Pbx-Dependent Pathways Govern the Coalescence and Fate of Motor Columns. Neuron. 2016 Sep 7;91(5):1005-1020
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8207399
Gene symbol: Hoxa5
Gene name: homeobox A5
Antibody: Anti-Hoxa5
Results
Specimen 1I Control rostral brachial: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-.
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted 1I Hoxa5 Control rostral brachial The expression domain was restricted to the rostral brachial region.
Specimen 1I PbxMNdelta rostral brachial: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted 1I Hoxa5 PbxMNdelta rostral brachial Hoxa5 rostro-caudal expression boundary is maintained in PbxMNdelta mutant.
Specimen 1I Control caudal brachial: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-.
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Ambiguous 1I Hoxa5 Control caudal brachial
Specimen 1I PbxMNdelta caudal brachial: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Ambiguous 1I Hoxa5 PbxMNdelta caudal brachial Hoxa5 rostro-caudal expression boundary is maintained in PbxMNdelta mutant.
Specimen S1C: embryonic day 11.5 (more )
Note: Double labeled: green - Hoxa5; red - Pbx3 (assay MGI:8206356).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region lateral motor column neuron Present Regionally restricted S1C Pbx3 Hoxa5 Expression was lateral motor column neurons in the rostral brachial region colocalized Pbx3.
Specimen S1I Control rostral: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: red - Hoxa5; green - Hoxc8 (assay MGI:8207401).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S1I Control rostral Hoxa5 marked the rostral brachial region.
Specimen S1I PbxMNdelta rostral: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Double labeled: red - Hoxa5; green - Hoxc8 (assay MGI:8207401).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S1I PbxMNdelta rostral Hoxa5 rostro-caudal expression boundary is maintained in PbxMNdelta mutant.
Specimen S1I Control middle: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: red - Hoxa5; green - Hoxc8 (assay MGI:8207401).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Weak Regionally restricted S1I Control
Specimen S1I PbxMNdelta middle: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Double labeled: red - Hoxa5; green - Hoxc8 (assay MGI:8207401).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Weak Regionally restricted S1I PbxMNdelta Hoxa5 rostro-caudal expression boundary is maintained in PbxMNdelta mutant.
Specimen S1I Control caudal: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: red - Hoxa5; green - Hoxc8 (assay MGI:8207401).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Ambiguous Regionally restricted S1I Control caudal Hoxa5 marked the rostral brachial region; caudal expression was not specified.
Specimen S1I PbxMNdelta caudal: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Double labeled: red - Hoxa5; green - Hoxc8 (assay MGI:8207401).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Ambiguous Regionally restricted S1I PbxMNdelta caudal Hoxa5 rostro-caudal expression boundary is maintained in PbxMNdelta mutant.
Specimen S1K Control right: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: red - Hoxa5; green - Hoxc8 (assay MGI:8207401); blue - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S1K Control Hoxa5 Isl1/2 Hoxc8 Hoxa5 marked the rostral brachial region.
Specimen S1K PbxMNdelta right: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: red - Hoxa5; green - Hoxc8 (assay MGI:8207401); blue - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S1K PbxMNdelta Hoxa5 Isl1/2 Hoxc8 Hoxa5 rostro-caudal expression boundary is maintained in PbxMNdelta mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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