Arx Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:178173 Mastracci TL, et al., Nkx2.2 and Arx genetically interact to regulate pancreatic endocrine cell development and endocrine hormone expression. Dev Biol. 2011 Nov 1;359(1):1-11
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:8161239
Gene symbol:	Arx
Gene name:	aristaless related homeobox

Antibody:	anti-Arx
Detection system:	Secondary antibody coupled to Cy3

Results

1B ©

Specimen 1B: embryonic day 13.5 (more

)
Note: Double labeled: red - Arx; green - Gcg (assay MGI:8161238).

Structure	Cell Type	Level	Pattern	Image	Note
TS21: pancreas	pancreatic A cell	Present		1B	A subset of positive cells coexpressed with Gcg in the alpha cells.

1C ©

Specimen 1C: embryonic day 13.5 (more

)
Note: Double labeled: red - Arx; green - Ghrl (assay MGI:8161241).

Structure	Cell Type	Level	Pattern	Image	Note
TS21: pancreas	pancreatic epsilon cell	Present		1C	A subset of positive cells coexpressed Ghrl.

1D ©

Specimen 1D: embryonic day 13.5; Nkx2-2^tm1Jlr/Nkx2-2^tm1Jlr (more

)
Note: Double labeled: red - Arx; green - Gcg (assay MGI:8161238).

Structure	Cell Type	Level	Pattern	Image	Note
TS21: pancreas	pancreatic A cell	Present		1D	Expressed in the few remaining alpha cells.

1E ©

Specimen 1E: embryonic day 13.5; Nkx2-2^tm1Jlr/Nkx2-2^tm1Jlr (more

)
Note: Double labeled: red - Arx; green - Ghrl (assay MGI:8161241).

Structure	Cell Type	Level	Pattern	Image	Note
TS21: pancreas	pancreatic epsilon cell	Present		1E	A subset of positive cells coexpressed with Ghrl. Increased number of Arx+ ghrelin cells, compared with the wild-type.

1G ©

Specimen 1G: embryonic day 10.5; Nkx2-2^tm3Suss/Nkx2-2⁺ (more

)
Note: Double labeled: red - Arx; green - Nkx2-2 reporter (assay MGI:8161244).

Structure	Level	Pattern	Image	Note
TS17: pancreas primordium	Present		1G	Coexpression with Nkx2-2 was seen.

1I ©

Specimen 1I: embryonic day 10.5; Nkx2-2^tm3Suss/Nkx2-2^tm3Suss (more

)
Note: Double labeled: red - Arx; green - Nkx2-2 reporter (assay MGI:8161244).

Structure	Level	Pattern	Image	Note
TS17: pancreas primordium	Present		1I	Coexpression with Nkx2-2 was seen.

2E ©

Specimen 2E: embryonic day 9.5 (more

)
Note: Double labeled: red - Arx; green - Pdx1 (assay MGI:8161245).

Structure	Level	Pattern	Image	Note
TS15: pancreas primordium	Present		2E	Compared with the Pdx1 and Gcg staining in adjacent section (Fig 2A), Arx+ cells that were glucagon+ as well as Pdx1+/Arx+ co-expressing cells that did not express glucagon were seen.

2F ©

Specimen 2F: embryonic day 11.5 (more

)
Note: Double labeled: red - Arx; green - Pdx1 (assay MGI:8161245).

Structure	Level	Pattern	Image	Note
TS19: pancreas primordium	Present		2F	Compared with the Pdx1 and Gcg staining in adjacent section (Fig 2A), Arx+ cells that were glucagon+ as well as Pdx1+/Arx+ co-expressing cells that did not express glucagon were seen.

2G ©

Specimen 2G: embryonic day 13.5 (more

)
Note: Double labeled: red - Arx; green - Pdx1 (assay MGI:8161245).

Structure	Level	Pattern	Image	Note
TS21: pancreas	Present		2G	Compared with the Pdx1 and Gcg staining in adjacent section (Fig 2A), Arx+ cells that were glucagon+ as well as Pdx1+/Arx+ co-expressing cells that did not express glucagon were seen.

2H ©

Specimen 2H: embryonic day 15.5 (more

)
Note: Double labeled: red - Arx; green - Pdx1 (assay MGI:8161245).

Structure	Level	Pattern	Image	Note
TS23: pancreas	Present		2H	Compared with the Pdx1 and Gcg staining in adjacent section (Fig 2A), Arx+ cells that were glucagon+ as well as Pdx1+/Arx+ co-expressing cells that did not express glucagon were seen.

2L ©

2N ©

Specimen 2L/N: embryonic day 15.5 (more

)
Note: Triple labeled: red - Arx; blue - Gcg (assay MGI:8161643); green - Ghrl (assay MGI:8161241).

Structure	Level	Pattern	Image	Note
TS23: pancreas	Present		2N , 2L	Expressed in ghrelin+ (dotted box) or glucagon+ single hormone+ cells, glucagon+/ghrelin+ co-expressing cells (arrow), and cells that do not express either hormone.

4M ©

Specimen 4M: postnatal day 0 (more

)
Note: Double labeled: red - Arx; green - Cpa1 (assay MGI:8161675).

Structure	Level	Pattern	Image	Note
TS27: pancreas	Present		4M

4N ©

Specimen 4N: postnatal day 0; Arx^tm1Gldn/?, Tg(Pdx1-cre)6Tuv/0 (more

)
Note: Conditional mutant. Arx^tm1Gldn/Arx^tm1Gldn in female, Arx^tm1Gldn/Y in male. Double labeled: red - Arx; green - Cpa1 (assay MGI:8161675).

Structure	Level	Pattern	Image	Note
TS27: pancreas	Absent		4N

4O ©

Specimen 4O: postnatal day 0; Nkx2-2^tm1Jlr/Nkx2-2^tm1Jlr (more

)
Note: Double labeled: red - Arx; green - Cpa1 (assay MGI:8161675).

Structure	Level	Pattern	Image	Note
TS27: pancreas	Present		4O

4P ©

Specimen 4P: postnatal day 0; Arx^tm1Gldn/?, Nkx2-2^tm1Jlr/Nkx2-2^tm1Jlr, Tg(Pdx1-cre)6Tuv/0 (more

)
Note: Conditional mutant. Arx^tm1Gldn/Arx^tm1Gldn in female, Arx^tm1Gldn/Y in male. Double labeled: red - Arx; green - Cpa1 (assay MGI:8161675).

Structure	Level	Pattern	Image	Note
TS27: pancreas	Absent		4P

S1A ©

Specimen S1A: embryonic day 11.5 (more

)
Note: Double labeled: red - Arx; blue - Gcg (assay MGI:8161643).

Structure	Cell Type	Level	Pattern	Image	Note
TS19: pancreas primordium	pancreatic A cell	Present		S1A	A subset of positive cells coexpressed with Gcg in the alpha cells.

S1B ©

Specimen S1B: embryonic day 13.5 (more

)
Note: Double labeled: red - Arx; blue - Gcg (assay MGI:8161643).

Structure	Cell Type	Level	Pattern	Image	Note
TS21: pancreas	pancreatic A cell	Present		S1B	A subset of positive cells coexpressed with Gcg in the alpha cells.

S1C ©

Specimen S1C: embryonic day 15.5 (more

)
Note: Double labeled: red - Arx; blue - Gcg (assay MGI:8161643).

Structure	Cell Type	Level	Pattern	Image	Note
TS23: pancreas	pancreatic A cell	Present		S1C	A subset of positive cells coexpressed with Gcg in the alpha cells.

S4A ©

Specimen S4A: postnatal day 0 (more

)
Note: Double labeled: red - Arx; green - Cpa1 (assay MGI:8161675).

Structure	Level	Pattern	Image	Note
TS27: pancreas	Present		S4A

S4B ©

Specimen S4B: postnatal day 0; Arx^tm1Gldn/?, Tg(Neurog3-cre)C1Able/0 (more

)
Note: Conditional mutant. Arx^tm1Gldn/Arx^tm1Gldn in female, Arx^tm1Gldn/Y in male. Double labeled: red - Arx; green - Cpa1 (assay MGI:8161675).

Structure	Level	Pattern	Image	Note
TS27: endocrine pancreas	Absent		S4B

S4C ©

Specimen S4C: postnatal day 0; Nkx2-2^tm1Jlr/Nkx2-2^tm1Jlr (more

)
Note: Double labeled: red - Arx; green - Cpa1 (assay MGI:8161675).

Structure	Level	Pattern	Image	Note
TS27: pancreas	Present		S4C

S4D ©

Specimen S4D: postnatal day 0; Arx^tm1Gldn/?, Nkx2-2^tm1Jlr/Nkx2-2^tm1Jlr, Tg(Neurog3-cre)C1Able/0 (more

)
Note: Conditional mutant. Arx^tm1Gldn/Arx^tm1Gldn in female, Arx^tm1Gldn/Y in male. Double labeled: red - Arx; green - Cpa1 (assay MGI:8161675).

Structure	Level	Pattern	Image	Note
TS27: endocrine pancreas	Absent		S4D

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