Reporter:	GFP
Antibody:	anti-GFP (600-101-215)
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	1a GFP/+	Expressed in subsets of cells.
TS28: retina outer nuclear layer	Present	Regionally restricted	1a GFP/+	Expressed in subsets of cells.

Structure	Level	Pattern	Image	Note
TS28: retina outer nuclear layer	Present		1a GFP/GFP	Expression intensity correlated with GFP dosage, with characteristic morphology of cone photoreceptors, with labeling in apical processes extending as far as the inner segment /outer segment boundary and basal processes terminating at the outer plexiform layer.
TS28: retina inner nuclear layer	Present		1a GFP/GFP	Expression intensity correlated with GFP dosage.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present		1a -/-;GFP/+
TS28: retina outer nuclear layer	Present		1a -/-;GFP/+	Increased expression compared with the GFP/+.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present		1a -/-;GFP/GFP	Expression intensity correlated with GFP dosage.
TS28: retina outer nuclear layer	Present		1a -/-;GFP/GFP	Expression intensity correlated with GFP dosage. Increased expression compared with the GFP/GFP.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	rod bipolar cell	Present		1b GFP , 1b merge	Coexpressed with PKC.

Structure	Level	Pattern	Image	Note
TS28: retina outer nuclear layer	Present	Graded	1c right	A ventral-to-dorsal gradient (high to low) was seen.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: retina outer nuclear layer	retinal cone cell	Present		2_1-4 , 2_1-5	Co-stained with pan-cone marker.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: retina outer nuclear layer	retinal cone cell	Present		2_2-4 , 2_2-5	Co-stained with pan-cone marker.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: photoreceptor layer outer segment	M cone cell	Trace		2_3-4 , 2_3-5	Low to undetectable in M opsin+ cells in the dorsal retina.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: photoreceptor layer outer segment	M cone cell	Present		2_4-5 , 2_4-4	Expression overlapped with M opsin-expressing cells in the central retina.

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer	Present		2_5-5 , 2_5-4	Little M-opsin+ cells in the ventral region.

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer	Weak		2_6-4 , 2_6-5	Low to undetectable in the dorsal retina.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: photoreceptor layer	S cone cell	Present		2_7-5 , 2_7-4	Expression overlapped with S opsin-expressing cells in the ventral retina.

Structure	Level	Pattern	Image	Note
TS21: retina layer	Present	Regionally restricted	3a GFP E13.5	Expressed in a central to peripheral gradient and localized to cells at the apical side of the retina, similar to the pattern of Ccdc136 transcript.

Structure	Level	Pattern	Image	Note
TS25: retina layer	Present	Regionally restricted	3a GFP E17.5	Mainly located within approximately 3 cell diameters from the apical surface.

Structure	Level	Pattern	Image	Note
TS27: retina layer	Present	Regionally restricted	3a GFP P0	Expressed in the apical side.

Structure	Level	Pattern	Image	Note
TS28: retina outer nuclear layer	Strong	Regionally restricted	3a GFP P4	Cone cell bodies, marked by intense GFP expression, changed from apical to more basal locations. Presence of cone axon terminals at the border of the outer plexiform layer was seen.

Structure	Level	Pattern	Image	Note
TS28: retina outer nuclear layer	Present	Regionally restricted	3a GFP P9	Cone cell bodies, marked by intense GFP expression, stayed more basal locations.

Structure	Level	Pattern	Image	Note
TS28: retina outer nuclear layer	Present	Regionally restricted	3a GFP P14	Positive cone cell bodies re-established their adult-like apical distribution.
TS28: retina inner nuclear layer	Present		3a GFP P14

Structure	Level	Pattern	Image	Note
TS23: retina	Present	Regionally restricted	3b GFP	Mainly located within approximately 3 cell diameters from the apical surface and exhibited a teardrop shaped morphology with an apically oriented process, with a similar pattern of condensed heterochromatin as in Crx+ cells.

Structure	Level	Pattern	Image	Note
TS28: retina outer nuclear layer	Present		3d P7 GFP , 3d P7 merge	Rxrg+GFP+ cells located in more basal positions.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: photoreceptor layer	retinal cone cell	Present		S3a merge dorsal , S3a GFP dorsal	Lower expression in the dorsal region, coexpressed with PNA and cone arrestin.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: photoreceptor layer	retinal cone cell	Present		S3a merge ventral , S3a GFP ventral	Higher expression in the ventral region, coexpressed with PNA and cone arrestin.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: retina outer nuclear layer	retinal cone cell	Present		S4a GFP-1 , S4a merge-1	Coexpressed with cone arrestin, similar as in the Nrl-/-.

Structure	Level	Pattern	Image	Note
TS28: retina outer nuclear layer	Present		S4a GFP-2 , S4a merge-2	PNA staining showed a shortened outer segment, similar as in Nrl-/-.

Structure	Level	Pattern	Image	Note
TS28: retina outer nuclear layer	Present		S4a GFP-3 , S4a merge-3	M opsin staining showed a shortened outer segment, similar as in Nrl-/-.

Structure	Level	Pattern	Image	Note
TS28: retina outer nuclear layer	Present		S4a GFP-4 , S4a merge-4	S opsin staining showed a shortened outer segment, similar as in Nrl-/-.

Structure	Level	Pattern	Image	Note
TS28: retina outer nuclear layer	Present		S4a merge-5 , S4a GFP-5	No Rhodopsin staining, similar as in Nrl-/-.

Structure	Level	Pattern	Image	Note
TS28: retina outer nuclear layer	Present	Regionally restricted	S5_upper left	Positive cones in the central retina migrate basally, low expression cells were also seen.
TS28: retina inner nuclear layer	Weak	Regionally restricted	S5_upper left	Expressed in the apical region.

Structure	Level	Pattern	Image	Note
TS28: retina outer nuclear layer	Present	Regionally restricted	S5_upper right	Positive cells located apically in the peripheral retina.

Structure	Level	Pattern	Image	Note
TS28: retina outer nuclear layer	Present		S6_P6

Structure	Level	Pattern	Image	Note
TS28: retina outer nuclear layer	Present		S6_p10
TS28: retina inner nuclear layer	Present	Single cells	S6_p10

Structure	Level	Pattern	Image	Note
TS28: retina outer nuclear layer	Present		S6_p12
TS28: retina inner nuclear layer	Present		S6_p12	More positive cells appeared.