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Gene Expression Data
Assay Details
Assay
Reference: J:146199 Barrionuevo F, et al., Testis cord differentiation after the sex determination stage is independent of Sox9 but fails in the combined absence of Sox9 and Sox8. Dev Biol. 2009 Mar 15;327(2):301-12
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7464303
Gene symbol: H2ax
Gene name: H2A.X variant histone
Antibody: anti-gammaH2AX (Ser139) (05-636)
Detection system: Secondary antibody
Assay notes: Fixation with 3.7% formaldehyde.
Results
Specimen 7Ba: postnatal day 12 (more )
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 7Ba Expression in wave of leptotene/zygotene spermatocytes.
Specimen 7Bb: postnatal day 12; Plekha5Tg(AMH-cre)1Flor/0, Sox8tm1Weg/Sox8+, Sox9tm1Gsr/Sox9tm1Gsr (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 7Bb Expression in wave of leptotene/zygotene spermatocytes.
Specimen 7Bc: postnatal day 12; Sox8tm1Weg/Sox8tm1Weg, Sox9tm1Gsr/Sox9tm1Gsr, Plekha5Tg(AMH-cre)1Flor/Plekha5+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: spermatocyte Weak 7Bc Reduced numbers of expressing cells compared to control.
Specimen 7Bd: postnatal day 12; Plekha5Tg(AMH-cre)1Flor/0, Sox8tm1Weg/Sox8+, Sox9tm1Gsr/Sox9tm1Gsr (more )
Note: Conditional mutant. Color inverted view.
Structure Level Pattern Image Note
TS28: spermatocyte Present 7Bd Expression in XY body in pachytene spermatocyte.
Specimen 7Be: postnatal day 12; Sox8tm1Weg/Sox8tm1Weg (more )
Note: Color inverted view.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Regionally restricted 7Be Expression in apoptotic cells.
Specimen 7Bf: postnatal day 12; Sox8tm1Weg/Sox8tm1Weg (more )
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Regionally restricted 7Bf Expression in dying metaphase cells at periphery of tubule.
Specimen 7Bg: postnatal day 18 (more )
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 7Bg Authors report synchronous onset of spermatogenesis. Diminished density of leptotene spermatocytes.
Specimen 7Bh: postnatal day 18; Plekha5Tg(AMH-cre)1Flor/0, Sox8tm1Weg/Sox8+, Sox9tm1Gsr/Sox9tm1Gsr (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Regionally restricted 7Bh Expression in aberrant cells in remaining tubules.
Specimen 7Bi: postnatal day 18; Sox8tm1Weg/Sox8tm1Weg, Sox9tm1Gsr/Sox9tm1Gsr, Plekha5Tg(AMH-cre)1Flor/Plekha5+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 7Bi Expression in XY body in pachytene spermatocytes.
TS28: seminiferous tubule Present Regionally restricted 7Bi Expression in apopoptic cells.
Specimen 7Bj: postnatal day 18; Sox8tm1Weg/Sox8tm1Weg, Sox9tm1Gsr/Sox9tm1Gsr, Plekha5Tg(AMH-cre)1Flor/Plekha5+ (more )
Note: Conditional mutant. Double labeled: green - H2ax; red - Mre11a (assay MGI:7464322).
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 7Bj Expression in XY body in pachytene spermatocytes.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory