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Gene Expression Data
Assay Details
Assay
Reference: J:225740 Snowball J, et al., Endodermal Wnt signaling is required for tracheal cartilage formation. Dev Biol. 2015 Sep 1;405(1):56-70
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7407287
Gene symbol: Sox9
Gene name: SRY (sex determining region Y)-box 9
Antibody: Anti-Sox9 (AB5535)
Detection system: Secondary antibody
Assay notes: Two anti-Sox9 antibodies were mentioned in the paper. It was not specified which one was used.
Results
Specimen 3A: embryonic day 13.5 (more )
Note: Double labeled: green - Sox9; red - Acta2 (assay MGI:7388675).
Structure Cell Type Level Pattern Image Note
TS21: trachea mesenchyme chondroblast Present Regionally restricted 3A Expressed as partial circumferential rings on the ventral side.
Specimen 3B: embryonic day 13.5; Shhtm1(EGFP/cre)Cjt/Shh+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: green - Sox9; red - Acta2 (assay MGI:7388675).
Structure Cell Type Level Pattern Image Note
TS21: trachea mesenchyme chondroblast Absent 3B
Specimen 3C: embryonic day 14.5 (more )
Note: Double labeled: green - Sox9; red - Acta2 (assay MGI:7388675).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme chondroblast Present Regionally restricted 3C Expressed in well-defined mesenchymal condensations.
TS22: vertebral cartilage condensation chondroblast Present 3C
Specimen 3D: embryonic day 14.5; Shhtm1(EGFP/cre)Cjt/Shh+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: green - Sox9; red - Acta2 (assay MGI:7388675).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme chondroblast Absent 3D
TS22: vertebral cartilage condensation chondroblast Present 3D
Specimen 3E: embryonic day 14.5 (more )
Note: Triple labeled: green - Sox9; blue - Nkx2-1 (assay MGI:7407288); red - Acta2 (assay MGI:7388675).
Structure Cell Type Level Pattern Image Note
TS22: vertebral cartilage condensation chondroblast Present 3E
TS22: trachea mesenchyme chondroblast Present Regionally restricted 3E Expressed in well-defined mesenchymal condensations.
Specimen 3E': embryonic day 14.5 (more )
Note: Triple labeled: green - Sox9; blue - Nkx2-1 (assay MGI:7407288); red - Acta2 (assay MGI:7388675).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme chondroblast Present Regionally restricted 3E' Expressed in well-defined mesenchymal condensations.
Specimen 3F: embryonic day 14.5; Shhtm1(EGFP/cre)Cjt/Shh+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Triple labeled: green - Sox9; blue - Nkx2-1 (assay MGI:7407288); red - Acta2 (assay MGI:7388675).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme chondroblast Absent 3F
TS22: vertebral cartilage condensation chondroblast Present 3F
Specimen 3F': embryonic day 14.5; Shhtm1(EGFP/cre)Cjt/Shh+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Triple labeled: green - Sox9; blue - Nkx2-1 (assay MGI:7407288); red - Acta2 (assay MGI:7388675).
Structure Level Pattern Image Note
TS22: trachea mesenchyme Present Scattered 3F' Randomly scattered throughout the dorsal mesenchyme (also Sox10+).
Specimen 4C: embryonic day 13.5 (more )
Note: Double labeled: green - Sox9; red - Nkx2-1 (assay MGI:7407319).
Structure Cell Type Level Pattern Image Note
TS21: trachea mesenchyme chondroblast Strong Regionally restricted 4C Expressed in mesenchymal condensations.
Specimen 4D: embryonic day 13.5; Shhtm1(EGFP/cre)Cjt/Shh+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: green - Sox9; red - Nkx2-1 (assay MGI:7407319).
Structure Level Pattern Image Note
TS21: trachea mesenchyme Weak Regionally restricted 4D Weakly detected in subepithelial mesenchyme.
Specimen 4E: embryonic day 13.5 (more )
Note: Double labeled: green - Sox9; red - Nkx2-1 (assay MGI:7407319).
Structure Level Pattern Image Note
TS21: lobar bronchus epithelium Strong Regionally restricted 4E Expressed in the cells of the peripheral lung overlapped with Nkx2-1.
Specimen 4F: embryonic day 13.5; Shhtm1(EGFP/cre)Cjt/Shh+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: green - Sox9; red - Nkx2-1 (assay MGI:7407319).
Structure Level Pattern Image Note
TS21: lobar bronchus epithelium Strong Regionally restricted 4F Expressed in the cells of the peripheral lung overlapped with Nkx2-1 as in the control.
Specimen 5A left control: embryonic day 11.5 (more )
Note: Double labeled: green - Sox9; red - Nkx2-1 (assay MGI:7407319).
Structure Level Pattern Image Note
TS19: trachea mesenchyme Present 5A left
Specimen 5A left cKO: embryonic day 11.5; Shhtm1(EGFP/cre)Cjt/Shh+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: green - Sox9; red - Nkx2-1 (assay MGI:7407319).
Structure Level Pattern Image Note
TS19: trachea mesenchyme Trace 5A middle Expression was nearly absent.
Specimen 5A right control: embryonic day 11.5 (more )
Note: Triple labeled: green - Sox9; purple - Nkx2-1 (assay MGI:7407326); red - Acta2 (assay MGI:7388675).
Structure Cell Type Level Pattern Image Note
TS19: trachea mesenchyme chondroblast Present Regionally restricted 5A right upper Expressed as partial circumferential rings on the ventral side.
Specimen 5A right cKO: embryonic day 11.5; Shhtm1(EGFP/cre)Cjt/Shh+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Triple labeled: green - Sox9; purple - Nkx2-1 (assay MGI:7407326); red - Acta2 (assay MGI:7388675).
Structure Level Pattern Image Note
TS19: trachea mesenchyme Trace 5A right lower Expression was nearly absent.
Specimen 5D upper control: embryonic day 13.5 (more )
Note: Double labeled: green - Sox9; red - BrdU.
Structure Cell Type Level Pattern Image Note
TS21: trachea mesenchyme chondroblast Present Regionally restricted 5D left upper Expressed as partial circumferential rings on the ventral side.
Specimen 5D upper cKO: embryonic day 13.5; Shhtm1(EGFP/cre)Cjt/Shh+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: green - Sox9; red - BrdU.
Structure Level Pattern Image Note
TS21: trachea mesenchyme Weak Regionally restricted 5D right upper Weakly detected in subepithelial mesenchyme. Cell proliferation was decreased in Sox9+ cells.
Specimen 6A: embryonic day 14.5 (more )
Note: Double labeled: green - Sox9; red - Acta2 (assay MGI:7388675).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme chondroblast Present Regionally restricted 6A Expressed as partial circumferential rings on the ventral side.
Specimen 6B: embryonic day 14.5; Sox9tm1Gsr/Sox9tm1Gsr, Tg(Col2a1-cre)1Bhr/0 (more )
Note: Conditional mutant. Double labeled: green - Sox9; red - Acta2 (assay MGI:7388675).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme chondroblast Weak Regionally restricted 6B Cartilage formation was severely impaired.
Specimen 6C: embryonic day 14.5 (more )
Note: Triple labeled: green - Sox9; purple - Nkx2-1 (assay MGI:7407326); red - Acta2 (assay MGI:7388675).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme chondroblast Present Regionally restricted 6C Expressed as partial circumferential rings on the ventral side.
Specimen 6D: embryonic day 14.5; Sox9tm1Gsr/Sox9tm1Gsr, Tg(Col2a1-cre)1Bhr/0 (more )
Note: Conditional mutant. Triple labeled: green - Sox9; purple - Nkx2-1 (assay MGI:7407326); red - Acta2 (assay MGI:7388675).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme chondroblast Weak Regionally restricted 6D Cartilage formation was severely impaired.
Specimen 6E: embryonic day 14.5 (more )
Note: Triple labeled: green - Sox9; purple - Nkx2-1 (assay MGI:7407326); red - Acta2 (assay MGI:7388675).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme chondroblast Present Regionally restricted 6E Expressed as partial circumferential rings on the ventral side.
Specimen 6F: embryonic day 14.5; Sox9tm1Gsr/Sox9tm1Gsr, Tg(Col2a1-cre)1Bhr/0 (more )
Note: Conditional mutant. Triple labeled: green - Sox9; purple - Nkx2-1 (assay MGI:7407326); red - Acta2 (assay MGI:7388675).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme chondroblast Weak Regionally restricted 6F Cartilage formation was severely impaired.
Specimen 6G: embryonic day 14.5 (more )
Note: Triple labeled: green - Sox9; purple - Nkx2-1 (assay MGI:7407326); red - Acta2 (assay MGI:7388675).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme chondroblast Present Regionally restricted 6G Expressed as partial circumferential rings on the ventral side.
Specimen 6H: embryonic day 14.5; Sox9tm1Gsr/Sox9tm1Gsr, Tg(Col2a1-cre)1Bhr/0 (more )
Note: Conditional mutant. Triple labeled: green - Sox9; purple - Nkx2-1 (assay MGI:7407326); red - Acta2 (assay MGI:7388675).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme chondroblast Weak Regionally restricted 6H Cartilage formation was severely impaired.
Specimen S3A/B control: embryonic day 18.5 (more )
Note: Double labeled: green - Sox9; red - Acta2 (assay MGI:7388675).
Structure Cell Type Level Pattern Image Note
TS26: trachea mesenchyme chondroblast Present Regionally restricted S3A left , S3B left Expressed as partial circumferential rings on the ventral side.
Specimen S3A/B cKO: embryonic day 18.5; Shhtm1(EGFP/cre)Cjt/Shh+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: green - Sox9; red - Acta2 (assay MGI:7388675).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium chondroblast Ambiguous S3A right , S3B right Virtually absent.
Specimen S5C: embryonic day 12.5 (more )
Structure Cell Type Level Pattern Image Note
TS20: trachea mesenchyme chondroblast Present S5C
Specimen S5D: embryonic day 12.5; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+, Twist2tm1.1(cre)Dor/Twist2+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS20: trachea mesenchyme chondroblast Present S5D Similar as in the control.
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