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Gene Expression Data
Assay Details
Assay
Reference: J:225740 Snowball J, et al., Endodermal Wnt signaling is required for tracheal cartilage formation. Dev Biol. 2015 Sep 1;405(1):56-70
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7388675
Gene symbol: Acta2
Gene name: actin alpha 2, smooth muscle, aorta
Antibody: Anti-smooth muscle alpha-Actin (clone 1A4)
Detection system: Secondary antibody
Results
Specimen 1B': embryonic day 11.5; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+, Shhtm1(EGFP/cre)Cjt/Shh+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Triple labeled: red - Acta2; green - Cre reporter.; blue - Sox9 (assay MGI:7388674).
Structure Level Pattern Image Note
TS19: trachea mesenchyme Present Regionally restricted 1B' Expressed on the dorsal side.
Specimen 3A: embryonic day 13.5 (more )
Note: Double labeled: red - Acta2; green - Sox9 (assay MGI:7407287).
Structure Cell Type Level Pattern Image Note
TS21: esophagus mesenchyme smooth muscle cell of the esophagus Present 3A
TS21: trachea mesenchyme smooth muscle cell of trachea Present Regionally restricted 3A Expressed on the dorsal side.
Specimen 3B: embryonic day 13.5; Shhtm1(EGFP/cre)Cjt/Shh+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: red - Acta2; green - Sox9 (assay MGI:7407287).
Structure Cell Type Level Pattern Image Note
TS21: esophagus mesenchyme smooth muscle cell of the esophagus Present 3B
TS21: trachea mesenchyme smooth muscle cell of trachea Present Widespread 3B Expression was expanded into the ventral region.
Specimen 3C: embryonic day 14.5 (more )
Note: Double labeled: red - Acta2; green - Sox9 (assay MGI:7407287).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme smooth muscle cell of trachea Present Regionally restricted 3C Expressed on the dorsal side.
Specimen 3D: embryonic day 14.5; Shhtm1(EGFP/cre)Cjt/Shh+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: red - Acta2; green - Sox9 (assay MGI:7407287).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme smooth muscle cell of trachea Present 3D Expression was expanded into the ventral region.
Specimen 3E: embryonic day 14.5 (more )
Note: Triple labeled: red - Acta2; blue - Nkx2-1 (assay MGI:7407288); green - Sox9 (assay MGI:7407287).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme smooth muscle cell of trachea Present Regionally restricted 3E Expressed on the dorsal side.
Specimen 3E': embryonic day 14.5 (more )
Note: Triple labeled: red - Acta2; blue - Nkx2-1 (assay MGI:7407288); green - Sox9 (assay MGI:7407287).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme smooth muscle cell of trachea Present Regionally restricted 3E' Expressed on the dorsal side. Transversal arrangements of the fibers were observed.
Specimen 3F: embryonic day 14.5; Shhtm1(EGFP/cre)Cjt/Shh+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Triple labeled: red - Acta2; blue - Nkx2-1 (assay MGI:7407288); green - Sox9 (assay MGI:7407287).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme smooth muscle cell of trachea Present 3F Expression was expanded into the ventral region.
Specimen 3F': embryonic day 14.5; Shhtm1(EGFP/cre)Cjt/Shh+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Triple labeled: red - Acta2; blue - Nkx2-1 (assay MGI:7407288); green - Sox9 (assay MGI:7407287).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme smooth muscle cell of trachea Present 3F' Expression was expanded into the ventral region. The orientation of muscle fibers was disrupted and organized parallel to the longitudinal axis of the trachea.
Specimen 5A right control: embryonic day 11.5 (more )
Note: Triple labeled: red - Acta2; purple - Nkx2-1 (assay MGI:7407326); green - Sox9 (assay MGI:7407287).
Structure Cell Type Level Pattern Image Note
TS19: trachea mesenchyme smooth muscle cell of trachea Present Regionally restricted 5A right upper Expressed on the dorsal side.
Specimen 5A right cKO: embryonic day 11.5; Shhtm1(EGFP/cre)Cjt/Shh+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Triple labeled: red - Acta2; purple - Nkx2-1 (assay MGI:7407326); green - Sox9 (assay MGI:7407287).
Structure Cell Type Level Pattern Image Note
TS19: trachea mesenchyme smooth muscle cell of trachea Absent 5A right lower
Specimen 5D lower control: embryonic day 13.5 (more )
Note: Double labeled: red - Acta2; pink - BrdU.
Structure Cell Type Level Pattern Image Note
TS21: trachea mesenchyme smooth muscle cell of trachea Present Regionally restricted 5D left lower Expressed on the dorsal side.
Specimen 5D lower cKO: embryonic day 13.5; Shhtm1(EGFP/cre)Cjt/Shh+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: red - Acta2; pink - BrdU.
Structure Cell Type Level Pattern Image Note
TS21: trachea mesenchyme smooth muscle cell of trachea Present 5D right lower Expression was expanded into the ventral region. Smooth muscle cell proliferation was increased in Acta2+ cells.
Specimen 6A: embryonic day 14.5 (more )
Note: Double labeled: red - Acta2; green - Sox9 (assay MGI:7407287).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme smooth muscle cell of trachea Present 6A
Specimen 6B: embryonic day 14.5; Sox9tm1Gsr/Sox9tm1Gsr, Tg(Col2a1-cre)1Bhr/0 (more )
Note: Conditional mutant. Double labeled: red - Acta2; green - Sox9 (assay MGI:7407287).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme smooth muscle cell of trachea Present 6B
Specimen 6C: embryonic day 14.5 (more )
Note: Triple labeled: red - Acta2; purple - Nkx2-1 (assay MGI:7407326); green - Sox9 (assay MGI:7407287).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme smooth muscle cell of trachea Present Regionally restricted 6C Expressed on the dorsal side.
Specimen 6D: embryonic day 14.5; Sox9tm1Gsr/Sox9tm1Gsr, Tg(Col2a1-cre)1Bhr/0 (more )
Note: Conditional mutant. Triple labeled: red - Acta2; purple - Nkx2-1 (assay MGI:7407326); green - Sox9 (assay MGI:7407287).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme smooth muscle cell of trachea Present Regionally restricted 6D Expressed on the dorsal side. No expansion of muscle into the ventral mesenchyme.
Specimen 6E: embryonic day 14.5 (more )
Note: Triple labeled: red - Acta2; purple - Nkx2-1 (assay MGI:7407326); green - Sox9 (assay MGI:7407287).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme smooth muscle cell of trachea Present Regionally restricted 6E Expressed on the dorsal side.
Specimen 6F: embryonic day 14.5; Sox9tm1Gsr/Sox9tm1Gsr, Tg(Col2a1-cre)1Bhr/0 (more )
Note: Conditional mutant. Triple labeled: red - Acta2; purple - Nkx2-1 (assay MGI:7407326); green - Sox9 (assay MGI:7407287).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme smooth muscle cell of trachea Present Regionally restricted 6F Expressed on the dorsal side. No expansion of muscle into the ventral mesenchyme.
Specimen 6G: embryonic day 14.5 (more )
Note: Triple labeled: red - Acta2; purple - Nkx2-1 (assay MGI:7407326); green - Sox9 (assay MGI:7407287).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme smooth muscle cell of trachea Present Regionally restricted 6G Expressed on the dorsal side.
Specimen 6H: embryonic day 14.5; Sox9tm1Gsr/Sox9tm1Gsr, Tg(Col2a1-cre)1Bhr/0 (more )
Note: Conditional mutant. Triple labeled: red - Acta2; purple - Nkx2-1 (assay MGI:7407326); green - Sox9 (assay MGI:7407287).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme smooth muscle cell of trachea Present Regionally restricted 6H Expressed on the dorsal side. No expansion of muscle into the ventral mesenchyme.
Specimen S3A/B control: embryonic day 18.5 (more )
Note: Double labeled: red - Acta2; green - Sox9 (assay MGI:7407287).
Structure Cell Type Level Pattern Image Note
TS26: trachea mesenchyme smooth muscle cell of trachea Present Regionally restricted S3A left , S3B left Expressed on the dorsal side.
TS26: esophagus mesenchyme smooth muscle cell of the esophagus Present S3B left , S3A left
Specimen S3A/B cKO: embryonic day 18.5; Shhtm1(EGFP/cre)Cjt/Shh+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: red - Acta2; green - Sox9 (assay MGI:7407287).
Structure Cell Type Level Pattern Image Note
TS26: trachea mesenchyme smooth muscle cell of trachea Present S3B right , S3A right Expression was expanded into the ventral region.
TS26: esophagus mesenchyme smooth muscle cell of the esophagus Present S3A right , S3B right
Specimen not shown: embryonic day 12.5; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+, Twist2tm1.1(cre)Dor/Twist2+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS20: trachea mesenchyme smooth muscle cell of trachea Present Similar as in the control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory