Sox9 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:225740 Snowball J, et al., Endodermal Wnt signaling is required for tracheal cartilage formation. Dev Biol. 2015 Sep 1;405(1):56-70
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7388674
Gene symbol:	Sox9
Gene name:	SRY (sex determining region Y)-box 9

Antibody:	Anti-Sox9 (AB5535)
Detection system:	Secondary antibody
Assay notes:	Two anti-Sox9 antibodies were mentioned in the paper. It was not specified which one was used.

Results

1B' ©

Specimen 1B': embryonic day 11.5; Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}/Gt(ROSA)26Sor⁺, Shh^{tm1(EGFP/cre)Cjt}/Shh⁺, Wls^tm1.1Lan/Wls^tm1.1Lan (more

)
Note: Conditional mutant. Triple labeled: blue - Sox9; green - Cre reporter; red - Acta2 (assay MGI:7388675).

Structure	Level	Pattern	Image	Note
TS19: trachea mesenchyme	Present		1B'

S5A ©

Specimen S5A: embryonic day 12.5 (more

)
Note: Triple labeled: blue - Sox9; green - Cre reporter; red - Nkx2-1 (assay MGI:7407319).

Structure	Level	Pattern	Image	Note
TS20: trachea mesenchyme	Present		S5A

S5B ©

Specimen S5B: embryonic day 12.5; Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}/Gt(ROSA)26Sor⁺, Twist2^{tm1.1(cre)Dor}/Twist2⁺, Wls^tm1.1Lan/Wls^tm1.1Lan (more

)
Note: Conditional mutant. Triple labeled: blue - Sox9; green - Cre reporter; red - Nkx2-1 (assay MGI:7407319).

Structure	Level	Pattern	Image	Note
TS20: trachea mesenchyme	Present		S5B	Similar as in the control.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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