Twist2^{tm1.1(cre)Dor}
Targeted Allele Detail

Summary

• Symbol: Twist2^{tm1.1(cre)Dor}
• Name: twist basic helix-loop-helix transcription factor 2; targeted mutation 1.1, David M Ornitz
• MGI ID: MGI:3830850
• Synonyms: dermo-1^Cre
• Gene: Twist2 Location: Chr1:91729183-91775750 bp, + strand Genetic Position: Chr1, 46.24 cM
• Alliance: Twist2^{tm1.1(cre)Dor} page

Vascular defects in Pten^tm1Hwu/Pten^tm1Hwu Twist2^{tm1.1(cre)Dor}/Twist2⁺ embryos

Show the 5 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81485
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutations: Insertion, Intragenic deletion
• Twist2^{tm1.1(cre)Dor} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Twist2 (MGI:104685)

• Mutation details: A targeting vector was designed to replace the first exon of the targeted gene with a cre cDNA sequence and an frt-flanked PGK-neo cassette. The construct was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Chimeric mice were bred to generate mutant mice. Mutant mice were then bred to FLPe mice (C57 genetic background) to remove the selection cassette. (J:90391)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Twist2 (mouse) Summary of all recombinase alleles driven by Twist2. Comparative matrix view of recombinase activities Alliance mouse Twist2 gene page

Disease models

Expression

In Mice Carrying this Mutation:

177 assay results 6 RNA-Seq or microarray experiment(s)

Tumor Data

List all tumor models in MMHCdb carrying Twist2^{tm1.1(cre)Dor}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Twist2 Mutation: 10 strains or lines available

References

• Original: J:81485 Sosic D, et al., Twist regulates cytokine gene expression through a negative feedback loop that represses NF-kappaB activity. Cell. 2003 Jan 24;112(2):169-80
• All: 91 reference(s)