About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:321982 De A, et al., The beta8 integrin cytoplasmic domain activates extracellular matrix adhesion to promote brain neurovascular development. Development. 2022 Mar 15;149(6):dev200472
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7281811
Gene symbol: Nes
Gene name: nestin
Antibody: Anti-Nestin (CH23001)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 3D: embryonic day 14.5 (more )
Note: Double labeled: green - Nes; red - Pecam1 (assay MGI:7281812).
Structure Level Pattern Image Note
TS22: ganglionic eminence Present 3D Expression was detected in neuroepithelial cells. Staining indicated Pecam+ blood vessels closely juxtapose Nes+ neuroepithelium.
Specimen 3E: embryonic day 14.5; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: green - Nes; red - Pecam1 (assay MGI:7281812).
Structure Level Pattern Image Note
TS22: ganglionic eminence Present 3E Expression was detected in neuroepithelial cells. Staining indicated abnormal, distended blood vessel morphologies as well as disrupted contacts with perivascular neuroepithelial cells.
Specimen 3F: embryonic day 14.5; Itgb8tm1Lfr/Itgb8tm1Lfr (more )
Note: Double labeled: green - Nes; red - Pecam1 (assay MGI:7281812).
Structure Level Pattern Image Note
TS22: ganglionic eminence Present 3F Expression was detected in neuroepithelial cells. Staining indicated abnormal, distended blood vessel morphologies as well as disrupted contacts with perivascular neuroepithelial cells.
Specimen S5A cortex: embryonic day 18.5; Tg(Nes-cre)1Kln/0 (more )
Note: Double labeled: green - Nes; red - Pecam1 (assay MGI:7281812).
Structure Level Pattern Image Note
TS26: cerebral cortex Present S5A Cortex Expression was detected in neuroepithelial cells.
Specimen S5A midbrain: embryonic day 18.5; Tg(Nes-cre)1Kln/0 (more )
Note: Double labeled: green - Nes; red - Pecam1 (assay MGI:7281812).
Structure Level Pattern Image Note
TS26: midbrain Present S5A Midbrain Expression was detected in neuroepithelial cells.
Specimen S5B cortex: embryonic day 18.5; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: green - Nes; red - Pecam1 (assay MGI:7281812).
Structure Level Pattern Image Note
TS26: cerebral cortex Present S5B Cortex Expression was detected in neuroepithelial cells. E18 mutants continued defects in the juxtaposition between Pecam+ endothelial cells and Nes+ neuroepithelial cells.
Specimen S5B midbrain: embryonic day 18.5; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: green - Nes; red - Pecam1 (assay MGI:7281812).
Structure Level Pattern Image Note
TS26: midbrain Present S5B Midbrain Expression was detected in neuroepithelial cells. E18 mutants continued defects in the juxtaposition between Pecam+ endothelial cells and Nes+ neuroepithelial cells.
Specimen S5C cortex: embryonic day 18.5; Itgb8tm1Lfr/Itgb8tm1Lfr (more )
Note: Double labeled: green - Nes; red - Pecam1 (assay MGI:7281812).
Structure Level Pattern Image Note
TS26: cerebral cortex Present S5C Cortex Expression was detected in neuroepithelial cells.
Specimen S5C midbrain: embryonic day 18.5; Itgb8tm1Lfr/Itgb8tm1Lfr (more )
Note: Double labeled: green - Nes; red - Pecam1 (assay MGI:7281812).
Structure Level Pattern Image Note
TS26: midbrain Present S5C Midbrain Expression was detected in neuroepithelial cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory