Probe:	Panx1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: embryo	Present		2a

Structure	Level	Pattern	Image	Note
TS17: embryo	Present		2b

Structure	Level	Pattern	Image	Note
TS19: embryo	Present		2c

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Present	Regionally restricted	3b	Expressed in the telencephalic vesicle wall.
TS15: future midbrain	Present	Regionally restricted	3b	Expressed in the basal plate of the mesencephalic vesicle.
TS15: 1st branchial arch	Present		3b
TS15: facio-acoustic preganglion complex	Present		3b
TS15: forelimb bud	Present		3b
TS15: trigeminal preganglion	Present		3b
TS15: 2nd branchial arch	Absent		3b
TS15: otocyst	Present		3b	Less intense expression.
TS15: neural tube	Present	Regionally restricted	3b	Expressed in the occipito-cervical and the truncal region.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted	4a	Expressed in the telencephalic vesicle.
TS17: midbrain	Present	Regionally restricted	4a	Expressed in the basal plate of the mesencephalic vesicle.
TS17: acoustic ganglion	Present		4a	More intense labeling.
TS17: facial ganglion	Present		4a	More intense labeling.
TS17: otocyst	Present		4a	More intense labeling.
TS17: trigeminal ganglion	Present		4a	More intense labeling.
TS17: forelimb bud	Present		4a
TS17: hindlimb bud	Present		4a
TS17: neural tube	Present	Regionally restricted	4a	Expressed in the truncal region.

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present	Regionally restricted	4b	Expressed in the mesencephalic vesicle wall.
TS17: hindbrain	Present	Regionally restricted	4b	Expressed in the rhombencephalic vesicle wall.
TS17: neural tube	Present		4b
TS17: rhombomere 1	Present		4b
TS17: rhombomere 2	Present		4b
TS17: rhombomere 3	Present		4b
TS17: rhombomere 4	Present		4b
TS17: rhombomere 5	Present		4b
TS17: rhombomere 6	Present		4b
TS17: rhombomere 7	Present		4b
TS17: rhombomere 8	Present		4b

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	5a	Expressed in the telencephalic vesicle wall, more intense than that at earlier stages.
TS19: midbrain	Present	Regionally restricted	5a	Expressed in the basal plate of the mesencephalic vesicle.
TS19: acoustic ganglion	Present		5a
TS19: dorsal root ganglion	Present		5a
TS19: facial ganglion	Present		5a
TS19: otocyst	Present		5a
TS19: trigeminal ganglion	Present		5a
TS19: future spinal cord	Present	Widespread	5a	Expressed in all spinal cord segments.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	5b	Expressed in the telencephalic vesicle wall.
TS19: midbrain	Present	Regionally restricted	5b	Expressed in the alar plate, more intense than that at earlier stages.

Structure	Level	Pattern	Image	Note
TS19: rhombic lip	Present	Widespread	5c	Expressed in the whole lip.
TS19: hindbrain ventricular layer	Present	Regionally restricted	5c	Expressed in the ventricular layer of the fourth ventricle.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	5d	Expressed in the telencephalic vesicle wall.
TS19: midbrain	Present	Regionally restricted	5d	Expressed in the basal plate of the mesencephalic vesicle.
TS19: acoustic ganglion	Present		5d
TS19: facial ganglion	Present		5d
TS19: trigeminal ganglion	Present		5d
TS19: otocyst	Present	Regionally restricted	5d	Expressed in the wall.
TS19: 1st branchial arch maxillary component	Present		5d
TS19: 2nd branchial arch	Absent		5d
TS19: mandible primordium	Absent		5d

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present		5e
TS19: forelimb bud	Present	Regionally restricted	5e	Expressed in the apical region.

Structure	Level	Pattern	Image	Note
TS19: acoustic ganglion	Present		6a'
TS19: facial ganglion	Present		6a'
TS19: trigeminal ganglion	Present		6a'
TS19: otocyst	Present	Regionally restricted	6a'	Expressed in the inner side of the wall.

Structure	Level	Pattern	Image	Note
TS19: inferior vagus ganglion	Present		6b'

Structure	Level	Pattern	Image	Note
TS19: 1st branchial arch	Present	Regionally restricted	6c'	Expressed in the apical part.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	6d'	Expressed in the inner side of the telencephalic vesicle wall.

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Present		6f
TS19: neural tube mantle layer	Present		6f

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present	Regionally restricted		Expressed in the alar lamina.
TS15: rhombomere 1	Absent
TS15: rhombomere 2	Absent
TS15: rhombomere 3	Absent
TS15: rhombomere 4	Absent
TS15: rhombomere 5	Absent
TS15: rhombomere 6	Absent
TS15: rhombomere 7	Absent
TS15: rhombomere 8	Absent
TS15: cranial preganglion	Present	Regionally restricted		No expression in the oculomotor motor neurons (III) and trochlear motor neurons (IV).

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present	Regionally restricted		Expressed in the alar lamina.
TS17: 1st branchial arch	Present
TS17: cranial ganglion	Present	Regionally restricted		Expressed in the oculomotor motor neurons (III) and trochlear motor neurons (IV).

Structure	Level	Pattern	Image	Note
TS19: metencephalon	Present
TS19: myelencephalon	Present
TS19: cranial ganglion	Present	Regionally restricted		Expressed in the oculomotor motor neurons (III) and trochlear motor neurons (IV).