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Gene Expression Data
Assay Details
Assay
Reference: J:290712 Ishiguro KI, et al., MEIOSIN Directs the Switch from Mitosis to Meiosis in Mammalian Germ Cells. Dev Cell. 2020 Feb 24;52(4):429-445.e10
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6721100
Gene symbol: Meiosin
Gene name: meiosis initiator
Antibody: Meiosin antibody
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Two Meiosin antibodies were described, but the one used in this assay was not detailed. Meiosin antibody MGI:6720904 may have been used in this assay.
Results
Specimen S1F: postnatal day 18 (more )
Note: Multi-labeled: green - Meiosin; red - Sycp3 (assay MGI:6721101); red - Stra8 (assay MGI:6721099); blue - DAPI.
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted S1F Meiosin , S1F Meiosin/Stra8 , S1F Meiosin/Sycp3 Expression localized mostly in nuclei of preleptotene spermatocytes in stage VII-VIII seminiferous tubules, largely co-localizing with Stra8. No expression in spermatocytes at other stages.
Specimen 1F left: postnatal week 8 (more )
Note: Triple labeled: green - Meiosin; red - Sycp3 (assay MGI:6721101); blue - DAPI.
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 1F (left) , 1F (left) Meiosin Expression localized mostly in nuclei of preleptotene spermatocytes in stage VII-VIII seminiferous tubules, largely co-localizing with Stra8. No expression in spermatocytes at other stages.
TS28: spermatid Absent 1F (left) , 1F (left) Meiosin No expression in postmeiotic spermatids, spermatogonia or Sertoli cells.
Specimen 1F left center: postnatal week 8 (more )
Note: Triple labeled: green - Meiosin; red - Stra8 (assay MGI:6721099); blue - DAPI.
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 1F (left center) Meiosin , 1F (left center) Expression localized mostly in nuclei of preleptotene spermatocytes in stage VII-VIII seminiferous tubules, largely co-localizing with Stra8. No expression in spermatocytes at other stages.
TS28: spermatid Absent 1F (left center) , 1F (left center) Meiosin No expression in postmeiotic spermatids, spermatogonia or Sertoli cells.
Specimen 1F center right: postnatal week 8 (more )
Note: Triple labeled: green - Meiosin; red - EdU; blue - DAPI.
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 1F (center right) , 1F (center right) Meiosin Expression localized mostly in nuclei of preleptotene spermatocytes in stage VII-VIII seminiferous tubules, largely co-localizing with Stra8. No expression in spermatocytes at other stages.
TS28: spermatid Absent 1F (center right) , 1F (center right) Meiosin No expression in postmeiotic spermatids, spermatogonia or Sertoli cells.
Specimen 1F right: postnatal week 8 (more )
Note: Triple labeled: green - Meiosin; red - Sox9 (assay MGI:6721104); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 1F (right) , 1F (right) Meiosin Expression localized mostly in nuclei of preleptotene spermatocytes in stage VII-VIII seminiferous tubules, largely co-localizing with Stra8. No expression in spermatocytes at other stages.
TS28: Sertoli cell Sertoli cell Absent 1F (right) Meiosin , 1F (right) No expression in postmeiotic spermatids, spermatogonia or Sertoli cells.
Specimen 1G: postnatal adult (more )
Note: Triple labeled: green - Meiosin; red - Sycp3 (assay MGI:6721101); blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Regionally restricted 1G (merge) , 1G Meiosin Co-expression with Stra8 in limited area along tubules.
Specimen 1H E13.5: embryonic day 13.5 (more )
Note: Triple labeled: green - Meiosin; red - Stra8 (assay MGI:6721099); blue - Tra98 (Gcna assay MGI:6721105).
Structure Level Pattern Image Note
TS21: germ cell of ovary Present 1H E13.5 Meiosin , 1H E13.5 (merge)
Specimen 1H E14.5: embryonic day 14.5 (more )
Note: Triple labeled: green - Meiosin; red - Stra8 (assay MGI:6721099); blue - Tra98 (Gcna assay MGI:6721105).
Structure Level Pattern Image Note
TS22: germ cell of ovary Present 1H E14.5 Meiosin , 1H E14.5 (merge)
Specimen 1H E15.5: embryonic day 15.5 (more )
Note: Triple labeled: green - Meiosin; red - Stra8 (assay MGI:6721099); blue - DAPI.
Structure Level Pattern Image Note
TS23: germ cell of ovary Weak 1H E15.5 Meiosin , 1H E15.5 (merge) Gradual reduction at E15.5.
Specimen 2G WT: postnatal day 18 (more )
Note: Triple labeled: green - Meiosin; red - Stra8 (assay MGI:6721099); blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 2G WT
Specimen 2G Meiosin KO: postnatal day 18; Meiosinem1Keish/Meiosinem1Keish (more )
Note: Triple labeled: green - Meiosin; red - Stra8 (assay MGI:6721099); blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Absent 2G Meiosin KO
Specimen 2G Stra8 KO: postnatal day 18; Stra8em4Keish/Stra8em4Keish (more )
Note: Triple labeled: green - Meiosin; red - Stra8 (assay MGI:6721099); blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Regionally restricted 2G Stra8 KO Expression localized in nuclei of Stra8 KO mice.
Specimen 7A WT: embryonic day 13.5 (more )
Note: Triple labeled: red - Meiosin; green - Stra8 (assay MGI:6721099); blue - DAPI.
Structure Level Pattern Image Note
TS21: germ cell of ovary Present 7A WT (merge) , 7A WT Meiosin
Specimen 7A Meiosin KO: embryonic day 13.5; Meiosinem1Keish/Meiosinem1Keish (more )
Note: Triple labeled: red - Meiosin; green - Stra8 (assay MGI:6721099); blue - DAPI.
Structure Level Pattern Image Note
TS21: germ cell of ovary Absent 7A Meiosin KO Meiosin , 7A Meiosin KO (merge)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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