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Gene Expression Data
Assay Details
Assay
Reference: J:135698 Burlison JS, et al., Pdx-1 and Ptf1a concurrently determine fate specification of pancreatic multipotent progenitor cells. Dev Biol. 2008 Apr 1;316(1):74-86
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6695715
Gene symbol: Ptf1a
Gene name: pancreas specific transcription factor, 1a
Reporter: YFP
Antibody: anti-GFP
Results
Specimen 2 E10.5 YFP/+ (upper): embryonic day 10.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Structure Level Pattern Image Note
TS17: neural tube Present Uniform 2_E10.5 YFP/+ (upper)

Specimen 2 E10.5 YFP/+ (lower): embryonic day 10.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Present Uniform 2_E10.5 YFP/+ (lower)
TS17: pancreas primordium ventral bud Present Uniform 2_E10.5 YFP/+ (lower)

Specimen 2 E10.5 YFP/YFP (upper): embryonic day 10.5; Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Structure Level Pattern Image Note
TS17: neural tube Present 2_E10.5 YFP/YFP (upper)

Specimen 2 E10.5 YFP/YFP (lower): embryonic day 10.5; Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Present 2_E10.5 YFP/YFP (lower) Expression initiated in dorsal and ventral pancreatic buds at E10.5.
TS17: pancreas primordium ventral bud Present 2_E10.5 YFP/YFP (lower) Expression initiated in dorsal and ventral pancreatic buds at E10.5.

Specimen 2 E11.5 YFP/+ (upper): embryonic day 11.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Structure Level Pattern Image Note
TS19: pancreas primordium dorsal bud Strong 2_E11.5 YFP/+ (upper)
TS19: pancreas primordium ventral bud Strong 2_E11.5 YFP/+ (upper)

Specimen 2 E11.5 YFP/+ (lower): embryonic day 11.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Structure Level Pattern Image Note
TS19: pancreas primordium dorsal bud Strong 2_E11.5 YFP/+ (lower) strong / NS
TS19: pancreas primordium ventral bud Strong 2_E11.5 YFP/+ (lower) strong / NS

Specimen 2 E11.5 YFP/YFP (upper): embryonic day 11.5; Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Structure Level Pattern Image Note
TS19: pancreas primordium dorsal bud Present 2_E11.5 YFP/YFP (upper)

Specimen 2 E11.5 YFP/YFP (lower): embryonic day 11.5; Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Structure Level Pattern Image Note
TS19: pancreas primordium dorsal bud Present 2_E11.5 YFP/YFP (lower) Expression in both dorsal and ventral pancreatic buds at E11.5
TS19: pancreas primordium ventral bud Present 2_E11.5 YFP/YFP (lower) Expression in both dorsal and ventral pancreatic buds at E11.5

Specimen 2 E12.5 YFP/+: embryonic day 12.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Structure Level Pattern Image Note
TS20: pancreas epithelium Strong Widespread 2_E12.5 YFP/+ Expression throughout pancreatic epithelium upon fusion of dorsal and ventral pancreas at E12.5.

Specimen 2 E12.5 YFP/YFP: embryonic day 12.5; Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Structure Level Pattern Image Note
TS20: pancreas Present Regionally restricted 2_E12.5 YFP/YFP Expression in ventral pancreatic remnant disappears by E12.5.

Specimen 2 E13.5 YFP/+ (upper): embryonic day 13.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Structure Level Pattern Image Note
TS21: pancreatic acinus Strong Punctate 2_E13.5 YFP/+ (upper) At E13.5 expression is punctate, appearing as many clusters, denoting acinar restriction.

Specimen 2 E13.5 YFP/+ (lower): embryonic day 13.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Structure Level Pattern Image Note
TS21: pancreas Strong Punctate 2_E13.5 YFP/+ (lower) At E13.5 expression is punctate, appearing as many clusters, denoting acinar restriction.
TS21: pancreatic acinus Strong Punctate 2_E13.5 YFP/+ (lower) At E13.5 expression is punctate, appearing as many clusters, denoting acinar restriction.

Specimen 2 E13.5 YFP/YFP (upper): embryonic day 13.5; Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Structure Level Pattern Image Note
TS21: pancreas Present Regionally restricted 2_E13.5 YFP/YFP (upper) Expression in dorsal pancreatic remnant persists until E13.5.

Specimen 2 E13.5 YFP/YFP (lower): embryonic day 13.5; Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Structure Level Pattern Image Note
TS21: pancreas Present Regionally restricted 2_E13.5 YFP/YFP (lower) Expression in dorsal pancreatic remnant persists until E13.5.

Specimen 2 E15.5 YFP/+: embryonic day 15.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Structure Level Pattern Image Note
TS23: pancreatic acinus Very strong Regionally restricted 2_E15.5 YFP/+ Intensity of expression increased further, corresponding with expansion of exocrine cells.

Specimen 2 E15.5 YFP/YFP: embryonic day 15.5; Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Structure Level Pattern Image Note
TS23: pancreas Absent 2_E15.5 YFP/YFP Expression in dorsal pancreatic remnant no longer seen at E15.5.

Specimen adult not shown: postnatal adult; Ptf1atm2.1Mgn/Ptf1a+ (more )
Structure Level Pattern Image Note
TS28: pancreas Strong Widespread Authors report pancreas-wide expression similar to that observed in late embryogenesis.

Specimen 3I: embryonic day 10.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Ptf1atm2.1Mgn/Ptf1a+ (more )
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Present 3I Expression only in dorsal pancreatic remnant from E10.5 to E12.5.

Specimen 3J: embryonic day 12.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Ptf1atm2.1Mgn/Ptf1a+ (more )
Structure Level Pattern Image Note
TS20: pancreas Present Regionally restricted 3J Expression only in dorsal pancreatic remnant from E10.5 to E12.5.

Specimen 3K: embryonic day 10.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Present 3K Expression only in dorsal pancreatic remnant from E10.5 to E12.5.

Specimen 3L: embryonic day 12.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Structure Level Pattern Image Note
TS20: pancreas Present Regionally restricted 3L Expression only in dorsal pancreatic remnant from E10.5 to E12.5.

Specimen E13.5 LacZko/LacZko;YFP/+ not shown: embryonic day 13.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Ptf1atm2.1Mgn/Ptf1a+ (more )
Structure Level Pattern Image Note
TS21: pancreas Absent

Specimen E13.5 LacZko/LacZko;YFP/YFP not shown: embryonic day 13.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Structure Level Pattern Image Note
TS21: pancreas Absent

Specimen 5A YFP (upper): embryonic day 10.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Triple labeled: green - Ptf1a reporter; blue - Pdx1 (assay MGI:6695727); red - Gcg (assay MGI:6695729).
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Present Regionally restricted 5A YFP (upper) , 5A Merge (upper) Expression coincident with Pdx1 throughout dorsal pancreatic epithelium, except for first wave endocrine cells.
TS17: duodenum Absent 5A YFP (upper) , 5A Merge (upper)

Specimen 5A YFP (lower): embryonic day 10.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Triple labeled: green - Ptf1a reporter; blue - Pdx1 (assay MGI:6695727); red - Gcg (assay MGI:6695729).
Structure Level Pattern Image Note
TS17: pancreas primordium ventral bud Present Regionally restricted 5A YFP (lower) , 5A Merge (lower) Expression coincident with Pdx1 throughout ventral pancreatic epithelium, except for first wave endocrine cells.
TS17: duodenum Absent 5A Merge (lower) , 5A YFP (lower)
TS17: extrahepatic bile duct Absent 5A Merge (lower) , 5A YFP (lower) No expression in common bile duct.

Specimen 5C E13.5: embryonic day 13.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Triple labeled: green - Ptf1a reporter; blue - Pdx1 (assay MGI:6695727); red - Neurog3 (assay MGI:6695730).
Structure Level Pattern Image Note
TS21: pancreatic acinus Present 5C E13.5 Expression restricted to pro-acinar structures.

Specimen 5C E15.5: embryonic day 15.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Triple labeled: green - Ptf1a reporter; blue - Pdx1 (assay MGI:6695727); red - Neurog3 (assay MGI:6695730).
Structure Level Pattern Image Note
TS23: pancreatic acinus Present 5C E15.5 Expression restricted to pro-acinar structures.

Specimen 5D (middle): embryonic day 11.5; Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Note: Triple labeled: green - Ptf1a reporter; blue - Pdx1 (assay MGI:6695727); red - Neurog3 (assay MGI:6695730).
Structure Level Pattern Image Note
TS19: pancreas primordium dorsal bud Present Widespread 5D (middle) Expression thoughout dorsal pancreatic remnant.

Specimen 5D (right): embryonic day 11.5; Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Note: Double labeled: green - Ptf1a reporter; blue - Pdx1 (assay MGI:6695727).
Structure Level Pattern Image Note
TS19: pancreas primordium ventral bud Present 5D (right)

Specimen S1 YFP E9.5: embryonic day 9.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Double labeled: green - Ptf1a reporter; red - Ptf1a protein (assay MGI:6695726).
Structure Level Pattern Image Note
TS15: pancreas primordium dorsal bud Present Widespread S1_E9.5 YFP , S1_E9.5 Merge Expression throughout dorsal and ventral pancreatic buds.
TS15: pancreas primordium ventral bud Present Widespread S1_E9.5 YFP , S1_E9.5 Merge Expression throughout dorsal and ventral pancreatic buds.

Specimen S1 YFP E10.5: embryonic day 10.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Double labeled: green - Ptf1a reporter; red - Ptf1a protein (assay MGI:6695726).
Structure Level Pattern Image Note
TS17: pancreas primordium Present S1_E10.5 YFP , S1_E10.5 Merge

Specimen S1 YFP E11.5: embryonic day 11.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Double labeled: green - Ptf1a reporter; red - Ptf1a protein (assay MGI:6695726).
Structure Level Pattern Image Note
TS19: pancreas primordium Present S1_E11.5 YFP , S1_E11.5 Merge

Specimen S1 YFP E12.5: embryonic day 12.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Double labeled: green - Ptf1a reporter; red - Ptf1a protein (assay MGI:6695726).
Structure Level Pattern Image Note
TS20: pancreas Strong Regionally restricted S1_E12.5 Merge , S1_E12.5 YFP Intense expression co-localizing with that of Ptf1a, except for some weaker expression towards central part of pancreas epithelium.

Specimen S1 YFP E13.5: embryonic day 13.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Double labeled: green - Ptf1a reporter; red - Ptf1a protein (assay MGI:6695726).
Structure Level Pattern Image Note
TS21: pancreatic acinus Present S1_E13.5 YFP , S1_E13.5 Merge From E13.5 on, expression coincident with Ptf1a in all acinar cells.

Specimen S1 YFP E15.5: embryonic day 15.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Double labeled: green - Ptf1a reporter; red - Ptf1a protein (assay MGI:6695726).
Structure Level Pattern Image Note
TS23: pancreatic acinus Present S1_E15.5 YFP , S1_E15.5 Merge From E13.5 on, expression coincident with Ptf1a in all acinar cells.

Specimen S2A YFP: embryonic day 12.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Triple labeled: green - Ptf1a reporter; blue - Pdx1 (assay MGI:6695727); red - Ptf1a protein (assay MGI:6695726).
Structure Level Pattern Image Note
TS20: pancreas Present Regionally restricted S2A Merge , S2A YFP Expression in dorsal pancreas.

Specimen S2B YFP: embryonic day 12.5; Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Note: Triple labeled: green - Ptf1a reporter; blue - Pdx1 (assay MGI:6695727); red - Ptf1a protein (assay MGI:6695726).
Structure Level Pattern Image Note
TS20: pancreas Present Regionally restricted S2B Merge , S2B YFP Expression in dorsal pancreatic remnant.


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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/22/2024
MGI 6.24
The Jackson Laboratory