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Gene Expression Data
Assay Details
Assay
Reference: J:135698 Burlison JS, et al., Pdx-1 and Ptf1a concurrently determine fate specification of pancreatic multipotent progenitor cells. Dev Biol. 2008 Apr 1;316(1):74-86
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6695730
Gene symbol: Neurog3
Gene name: neurogenin 3
Antibody: anti-Ngn3
Results
Specimen 5B Ngn3: embryonic day 10.5 (more )
Note: Triple labeled: red - Neurog3; blue - Pdx1 (assay MGI:6695727); green - Ptf1a (assay MGI:109400).
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Present Regionally restricted 5B Ngn3 , 5B Merge Expression in pancreatic progenitor cells of dorsal pancreatic bud at E10.5 coinciding with Ptf1a and Pdx1 in scattered cells throughout pancreatic epithelium.
Specimen 5C E13.5: embryonic day 13.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Triple labeled: red - Neurog3; blue - Pdx1 (assay MGI:6695727); green - Ptf1a reporter (assay MGI:6695715).
Structure Level Pattern Image Note
TS21: pancreatic duct Present 5C E13.5 Expression in ductal cords.
TS21: pancreatic acinus Absent 5C E13.5 No expression in pro-acinar structures.
Specimen 5C E15.5: embryonic day 15.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Triple labeled: red - Neurog3; blue - Pdx1 (assay MGI:6695727); green - Ptf1a reporter (assay MGI:6695715).
Structure Level Pattern Image Note
TS23: pancreatic duct Present 5C E15.5 Expression in ductal cords.
TS23: pancreatic acinus Absent 5C E15.5 No expression in pro-acinar structures.
Specimen 5D (left,middle): embryonic day 11.5; Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Note: Triple labeled: red - Neurog3; blue - Pdx1 (assay MGI:6695727); green - Ptf1a reporter (assay MGI:6695715).
Structure Level Pattern Image Note
TS19: pancreas primordium dorsal bud Present Widespread 5D (left) , 5D (middle) Expression thoughout dorsal pancreatic remnant.
Specimen 6D: embryonic day 12.5 (more )
Note: Triple labeled: red - Neurog3; blue - Pdx1 (assay MGI:6695727); green - Gcg (assay MGI:6695729).
Structure Level Pattern Image Note
TS20: pancreas Present Regionally restricted 6D Expression dorsal pancreas.
Specimen 6E: embryonic day 12.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Triple labeled: red - Neurog3; blue - Pdx1 (assay MGI:6695727); green - Gcg (assay MGI:6695729).
Structure Level Pattern Image Note
TS20: pancreas epithelium Absent 6E
Specimen 6F: embryonic day 12.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Note: Triple labeled: red - Neurog3; blue - Pdx1 (assay MGI:6695727); green - Gcg (assay MGI:6695729).
Structure Level Pattern Image Note
TS20: pancreas epithelium Absent 6F
Specimen S2C Ngn3: embryonic day 10.5 (more )
Note: Triple labeled: red - Neurog3; blue - Pdx1 (assay MGI:6695727); green - Ptf1a (assay MGI:109400).
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Present S2C Merge , S2C Ngn3
Specimen S3C/D: embryonic day 9.5 (more )
Note: Triple labeled: red - Neurog3; blue - Pdx1 (assay MGI:6695727); green - Ptf1a (assay MGI:109400).
Structure Level Pattern Image Note
TS15: pancreas primordium Present S3C , S3D Expression in pre-pancreatic endoderm at E9.5.
Specimen S3E: embryonic day 9.5; Pdx1tm2Cvw/Pdx1+ (more )
Note: Triple labeled: red - Neurog3; blue - Pdx1 (assay MGI:6695727); green - Gcg (assay MGI:6695729).
Structure Level Pattern Image Note
TS15: pancreas primordium Present S3E
Specimen S3F: embryonic day 9.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Triple labeled: red - Neurog3; blue - Pdx1 (assay MGI:6695727); green - Gcg (assay MGI:6695729).
Structure Level Pattern Image Note
TS15: pancreas primordium Present S3F Expression in pre-pancreatic endoderm at E9.5.
Specimen S3G: embryonic day 9.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Note: Triple labeled: red - Neurog3; blue - Pdx1 (assay MGI:6695727); green - Gcg (assay MGI:6695729).
Structure Level Pattern Image Note
TS15: pancreas primordium Present S3G Expression in pre-pancreatic endoderm at E9.5.
Specimen S3H: embryonic day 10.5 (more )
Note: Triple labeled: red - Neurog3; blue - Pdx1 (assay MGI:6695727); green - Gcg (assay MGI:6695729).
Structure Level Pattern Image Note
TS17: pancreas primordium Present S3H
Specimen S3I: embryonic day 10.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Triple labeled: red - Neurog3; blue - Pdx1 (assay MGI:6695727); green - Gcg (assay MGI:6695729).
Structure Level Pattern Image Note
TS17: pancreas primordium Absent S3I No expression in pancreatic epithelium.
Specimen S3J: embryonic day 10.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Note: Triple labeled: red - Neurog3; blue - Pdx1 (assay MGI:6695727); green - Gcg (assay MGI:6695729).
Structure Level Pattern Image Note
TS17: pancreas primordium Absent S3J No expression in pancreatic epithelium.
Specimen S4C Ngn3: embryonic day 9.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Triple labeled: red - Neurog3; blue - Pdx1 (assay MGI:6695727); green - Ptf1a (assay MGI:109400).
Structure Level Pattern Image Note
TS15: foregut epithelium Present S4_Merge , S4_Ngn3
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory