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Gene Expression Data
Assay Details
Assay
Reference: J:135698 Burlison JS, et al., Pdx-1 and Ptf1a concurrently determine fate specification of pancreatic multipotent progenitor cells. Dev Biol. 2008 Apr 1;316(1):74-86
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6695727
Gene symbol: Pdx1
Gene name: pancreatic and duodenal homeobox 1
Antibody: anti-Pdx-1
Assay notes: Two antibodies were described, but the one used in this assay was not detailed. Anti-Pdx1 MGI:6695357 may have been used.
Results
Specimen 5A Pdx-1 (upper): embryonic day 10.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Triple labeled: blue - Pdx1; red - Gcg (assay MGI:6695729); green - Ptf1a reporter (assay MGI:6695715).
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Present Regionally restricted 5A Pdx-1 Gcg (upper) , 5A Merge (upper) Expression coincident with Ptf1a throughout dorsal pancreatic epithelium, except for first wave endocrine cells.
TS17: duodenum Present 5A Pdx-1 Gcg (upper) , 5A Merge (upper)
Specimen 5A Pdx-1 (lower): embryonic day 10.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Triple labeled: blue - Pdx1; red - Gcg (assay MGI:6695729); green - Ptf1a reporter (assay MGI:6695715).
Structure Level Pattern Image Note
TS17: pancreas primordium ventral bud Present Regionally restricted 5A Pdx-1 Gcg (lower) , 5A Merge (lower) Expression coincident with Ptf1a throughout ventral pancreatic epithelium, except for first wave endocrine cells.
TS17: duodenum Present 5A Pdx-1 Gcg (lower) , 5A Merge (lower)
TS17: extrahepatic bile duct Present 5A Pdx-1 Gcg (lower) , 5A Merge (lower)
Specimen 5B Pdx-1: embryonic day 10.5 (more )
Note: Triple labeled: blue - Pdx1; red - Neurog3 (assay MGI:6695730); green - Ptf1a (assay MGI:6695726).
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Present Regionally restricted 5B Pdx-1 , 5B Merge Expression in pancreatic progenitor cells of dorsal pancreatic bud at E10.5 coinciding with Ptf1a and Ngn3 in scattered cells throughout pancreatic epithelium.
Specimen E11.5 +/+ not shown: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: pancreas primordium Present Regionally restricted Expression still coincides with that of Ptf1a in almost all pancreatic epithelial cells.
Specimen 5C E13.5: embryonic day 13.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Triple labeled: blue - Pdx1; green - Ptf1a reporter (assay MGI:6695715); red - Neurog3 (assay MGI:6695730).
Structure Level Pattern Image Note
TS21: pancreatic duct Present 5C E13.5 Expression in ductal cords.
Specimen 5C E15.5: embryonic day 15.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Triple labeled: blue - Pdx1; green - Ptf1a reporter (assay MGI:6695715); red - Neurog3 (assay MGI:6695730).
Structure Level Pattern Image Note
TS23: pancreatic duct Present 5C E15.5 Expression in ductal cords.
Specimen 5D (left/middle): embryonic day 11.5; Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Note: Triple labeled: blue - Pdx1; green - Ptf1a reporter (assay MGI:6695715); red - Neurog3 (assay MGI:6695730).
Structure Level Pattern Image Note
TS19: pancreas primordium dorsal bud Present Widespread 5D (left) , 5D (middle) Expression thoughout dorsal pancreatic remnant.
Specimen 5D (right): embryonic day 11.5; Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Note: Double labeled: blue - Pdx1; green - Ptf1a reporter (assay MGI:6695715).
Structure Level Pattern Image Note
TS19: pancreas primordium dorsal bud Present Widespread 5D (right) Expression thoughout dorsal pancreatic remnant.
Specimen 6A: embryonic day 12.5 (more )
Note: Triple labeled: blue - Pdx1; green - Gcg (assay MGI:6695729); red - Ins1 (assay MGI:6695732) and Ins2 (assay MGI:6695733).
Structure Level Pattern Image Note
TS20: pancreas epithelium Present 6A
Specimen 6B: embryonic day 12.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Triple labeled: blue - Pdx1; green - Gcg (assay MGI:6695729); red - Ins1 (assay MGI:6695732) and Ins2 (assay MGI:6695733).
Structure Level Pattern Image Note
TS20: pancreas epithelium Present 6B
Specimen 6C: embryonic day 12.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Note: Triple labeled: blue - Pdx1; green - Gcg (assay MGI:6695729); red - Ins1 (assay MGI:6695732) and Ins2 (assay MGI:6695733).
Structure Level Pattern Image Note
TS20: pancreas epithelium Present 6C
Specimen 6D: embryonic day 12.5 (more )
Note: Triple labeled: blue - Pdx1; green - Gcg (assay MGI:6695729); red - Neurog3 (assay MGI:6695730).
Structure Level Pattern Image Note
TS20: pancreas epithelium Present 6D
Specimen 6E: embryonic day 12.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Triple labeled: blue - Pdx1; green - Gcg (assay MGI:6695729); red - Neurog3 (assay MGI:6695730).
Structure Level Pattern Image Note
TS20: pancreas epithelium Present 6E
Specimen 6F: embryonic day 12.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Note: Triple labeled: blue - Pdx1; green - Gcg (assay MGI:6695729); red - Neurog3 (assay MGI:6695730).
Structure Level Pattern Image Note
TS20: pancreas epithelium Present 6F
Specimen S2A Pdx-1: embryonic day 12.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Triple labeled: blue - Pdx1; red - Pdf1a (assay MGI:6695726); green - Ptf1a reporter (assay MGI:6695715).
Structure Level Pattern Image Note
TS20: pancreas epithelium Present Regionally restricted S2A Pdx-1 , S2A Merge Expression in dorsal pancreas. Expression in multipotent progenitor cells (MPCs) at distal tips of pancreatic epithelium.
Specimen S2B Pdx-1: embryonic day 12.5; Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Note: Triple labeled: blue - Pdx1; red - Pdf1a (assay MGI:6695726); green - Ptf1a reporter (assay MGI:6695715).
Structure Level Pattern Image Note
TS20: pancreas Present Regionally restricted S2B Merge , S2B Pdx-1 Expression in dorsal pancreatic remnant.
Specimen S2C Pdx-1: embryonic day 10.5 (more )
Note: Triple labeled: blue - Pdx1; red - Neurog3 (assay MGI:6695730); green - Ptf1a (assay MGI:6695726).
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Present Regionally restricted S2C Merge , S2C Pdx-1 present / RR
Specimen S3A/D Pdx-1: embryonic day 9.5; Pdx1tm2Cvw/Pdx1+, Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Triple labeled: blue - Pdx1; red - Neurog3 (assay MGI:6695730); green - Ptf1a (assay MGI:6695726).
Structure Level Pattern Image Note
TS15: pancreas primordium Present Regionally restricted S3A , S3D Expression coincide with that of Ptf1a in vast majority of pancreatic epithelial cells from E9.5 to E10.5.
Specimen S3E: embryonic day 9.5; Pdx1tm2Cvw/Pdx1+ (more )
Note: Triple labeled: blue - Pdx1; green - Gcg (assay MGI:6695729); red - Neurog3 (assay MGI:6695730).
Structure Level Pattern Image Note
TS15: pancreas primordium Present S3E
Specimen S3F: embryonic day 9.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Triple labeled: blue - Pdx1; green - Gcg (assay MGI:6695729); red - Neurog3 (assay MGI:6695730).
Structure Level Pattern Image Note
TS15: pancreas primordium Present S3F
Specimen S3G: embryonic day 9.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Note: Triple labeled: blue - Pdx1; green - Gcg (assay MGI:6695729); red - Neurog3 (assay MGI:6695730).
Structure Level Pattern Image Note
TS15: pancreas primordium Present S3G
Specimen S3H: embryonic day 10.5 (more )
Note: Triple labeled: blue - Pdx1; red - Pdf1a (assay MGI:6695726); green - Ptf1a reporter (assay MGI:6695715).
Structure Level Pattern Image Note
TS17: pancreas primordium Present S3H
Specimen S3I: embryonic day 10.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Triple labeled: blue - Pdx1; red - Pdf1a (assay MGI:6695726); green - Ptf1a reporter (assay MGI:6695715).
Structure Level Pattern Image Note
TS17: pancreas primordium Present S3I
Specimen S3J: embryonic day 10.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Structure Level Pattern Image Note
TS17: pancreas primordium Present S3J
Specimen S4 Pdx-1: embryonic day 9.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Triple labeled: blue - Pdx1; red - Neurog3 (assay MGI:6695730); green - Ptf1a (assay MGI:6695726).
Structure Level Pattern Image Note
TS15: foregut epithelium Present S4_Pdx-1 , S4_Merge
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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